Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,829,978 Δ1 bp 34.5% coding (1471/1479 nt) astD ← succinylglutamic semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,829,9780C.34.5% 63.5 / 29.3 29coding (1471/1479 nt)astDsuccinylglutamic semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (11/8);  new base . (6/4);  total (17/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGC  >  W3110S.gb/1829921‑1830046
                                                         |                                                                    
tAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGa                                                          >  1:4100599/1‑70 (MQ=255)
tAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGa                                                          >  1:1761465/1‑70 (MQ=255)
  atgatgCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGaaa                                                        <  1:2736818/70‑1 (MQ=255)
  atgatgCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCA‑CACCTCATCGGaaaaa                                                      >  1:1922558/1‑71 (MQ=255)
  atgatgCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCA‑CACCTCATCGGaaaaa                                                      >  1:1928591/1‑71 (MQ=255)
     atgCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATc                                                    <  1:1226616/71‑1 (MQ=255)
              cccTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCggg                                           >  1:1946013/1‑71 (MQ=255)
              cccTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCggg                                           >  1:3348076/1‑71 (MQ=255)
                cTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGaa                                                         <  1:1405082/55‑1 (MQ=255)
                   ccAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCA‑CACCTCATCGGAAAAATCCAGCGCGGGGTTaa                                      <  1:2706123/70‑1 (MQ=255)
                     aGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGaaa                                                        >  1:3131207/1‑51 (MQ=255)
                     aGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGaaa                                                        >  1:3840567/1‑51 (MQ=255)
                     aGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGaaa                                                        >  1:3453650/1‑51 (MQ=255)
                      gCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCg                                   <  1:3277315/71‑1 (MQ=255)
                         cGTCGAAATTGACTTCCCAGGCGTTCATCGCA‑CACCTCATCGGaaaa                                                       <  1:1084977/47‑1 (MQ=255)
                         cGTCGAAATTGACTTCCCAGGCGTTCATCGCA‑CACCTCATCGGaaaa                                                       <  1:123957/47‑1 (MQ=255)
                           tCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAg                                     <  1:4764243/64‑1 (MQ=255)
                            cGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATc                                                    >  1:4774227/1‑48 (MQ=255)
                            cGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATc                                                    >  1:2616954/1‑48 (MQ=255)
                             gAAATTGACTTCCCAGGCGTTCATCGCA‑CACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCggg                            >  1:2180896/1‑70 (MQ=255)
                              aaaTTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGc                           <  1:2962480/71‑1 (MQ=255)
                                aTTGACTTCCCAGGCGTTCATCGCA‑CACCTCATCGGAAAAATc                                                    >  1:3347998/1‑43 (MQ=39)
                                  tGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAAt                                                     >  1:1772777/1‑41 (MQ=255)
                                   gACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGt                      >  1:587283/1‑71 (MQ=255)
                                    aCTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGtt                     <  1:3947146/71‑1 (MQ=255)
                                        cccAGGGGTTCATCGCA‑CACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAAc                  >  1:988578/1‑69 (MQ=255)
                                        cccAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAAc                  <  1:3366010/70‑1 (MQ=255)
                                        cccAGGCGTTCATCGCA‑CACCTCATCGGAAAAATCCAGcc                                               >  1:1961217/1‑40 (MQ=39)
                                                   aTCGCA‑CACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCa     <  1:4590081/71‑1 (MQ=255)
                                                       caccacCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGc  <  1:664830/71‑1 (MQ=255)
                                                         |                                                                    
TAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGC  >  W3110S.gb/1829921‑1830046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑