| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,785,165:1 | +T | 12.8% | coding (475/1701 nt) | ydiD → | short chain acyl‑CoA synthetase, anaerobic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,785,165 | 1 | . | T | 12.8% | 152.9 / 24.7 | 47 | coding (475/1701 nt) | ydiD | short chain acyl‑CoA synthetase, anaerobic |
| Reads supporting (aligned to +/- strand): ref base . (26/15); new base T (3/3); total (29/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
AATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGCCA > W3110S.gb/1785099‑1785234 | aaTAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAA‑TCCt > 1:551624/1‑71 (MQ=255) aGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCa > 1:1173137/1‑70 (MQ=255) aGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCa > 1:3379237/1‑70 (MQ=255) aGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCa > 1:2279394/1‑70 (MQ=255) gCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATTGCTGCCGCTGCaaa < 1:1845036/71‑1 (MQ=255) aaaTGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAg > 1:1941211/1‑71 (MQ=255) aTGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCaaaa > 1:2045179/1‑65 (MQ=255) aTGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCaaaa > 1:2342070/1‑65 (MQ=255) aTGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCt > 1:437320/1‑71 (MQ=255) tGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCcac < 1:3273478/68‑1 (MQ=255) gCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCcac < 1:5054265/67‑1 (MQ=255) gCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAAc > 1:5106757/1‑70 (MQ=255) gCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAAc > 1:1387152/1‑70 (MQ=255) aCGTTGTTTAAACAAACGCGTCCGGTAGATTTAATTCCTGCCGCTGCaaa < 1:3587360/50‑1 (MQ=39) aCGTTGTTTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTc < 1:2674570/58‑1 (MQ=255) aCGTTGTTTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTacaac > 1:754935/1‑70 (MQ=255) aCGTTGTTTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTacaac > 1:4622074/1‑70 (MQ=255) cGTTGTTTAAACAAACTCGTCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTacaaca > 1:1517466/1‑70 (MQ=255) gttgttTAAACAAACGCGTCCGGTAGATTTAATTCCTGCCGCTGCAAAATCAGCTTCCACAACTacaaca > 1:127006/1‑70 (MQ=255) gttgttTAAACAAACGCGTCCGGTAGATTTAATTCCTGCCGCTGCAAAATCAGCTTCCACAACTacaaca > 1:3517031/1‑70 (MQ=255) gttgttTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACaaa < 1:2827606/71‑1 (MQ=255) gttgttTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACaaa > 1:698363/1‑71 (MQ=255) tgttTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCcac < 1:1013923/57‑1 (MQ=255) tttAAACAAACGCGTCCGGTAGATTTAATTCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAAtt < 1:1641900/70‑1 (MQ=255) aaacGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCa > 1:1483161/1‑40 (MQ=255) aaacGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACaa > 1:3357813/1‑50 (MQ=255) tCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAAt < 1:844247/54‑1 (MQ=255) tCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAAt < 1:3195919/54‑1 (MQ=255) tCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAAt < 1:3607944/54‑1 (MQ=255) ccGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACaaa > 1:4708695/1‑69 (MQ=255) ggTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTgg < 1:690662/71‑1 (MQ=255) aGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACaaa > 1:85741/1‑64 (MQ=255) aGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTc > 1:333577/1‑71 (MQ=255) gATTTAATTCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTc > 1:846837/1‑71 (MQ=255) aTTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAAtt < 1:4222609/47‑1 (MQ=255) aTTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACaa < 1:18060/61‑1 (MQ=255) aTTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACaa < 1:4069147/61‑1 (MQ=255) aTTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACaa < 1:1733217/61‑1 (MQ=255) ttAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCg > 1:4236564/1‑49 (MQ=255) ttAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTg < 1:5038461/54‑1 (MQ=255) ttAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGc > 1:586794/1‑71 (MQ=255) ttAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGc > 1:903505/1‑71 (MQ=255) ttAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGc > 1:1766332/1‑71 (MQ=255) ttAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGc > 1:3027286/1‑71 (MQ=255) ttAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGAAAAACTGGCTCCCGc > 1:4151131/1‑71 (MQ=255) ttAA‑CCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGc > 1:1901279/1‑71 (MQ=255) tAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGc < 1:737615/70‑1 (MQ=255) aa‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGt > 1:4313577/1‑45 (MQ=255) aa‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGt > 1:1857382/1‑45 (MQ=255) aa‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGt > 1:3095328/1‑45 (MQ=255) aa‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGCCa > 1:1740194/1‑71 (MQ=255) aa‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGCCa > 1:3894893/1‑71 (MQ=255) aa‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGCCa > 1:3628112/1‑71 (MQ=255) aa‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGCCa > 1:2708681/1‑71 (MQ=255) | AATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAA‑TCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGCCA > W3110S.gb/1785099‑1785234 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |