New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb 4177036 =84 (1.580)4 (0.090) 3/86 NT 4.9% coding (459/984 nt) gspK general secretory pathway component, cryptic
?W3110S.gb 4177058 = 80 (1.710)coding (437/984 nt) gspK general secretory pathway component, cryptic
Rejected: Frequency below/above cutoff threshold.

CGCTGCTGATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATTGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/4177109‑4177036
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑attgatTGCACGATCTCATCGGTGTTTCCGCGATCGATACCGGCGTTAATCAGCAGCGCACTGAATACCTGGG  >  W3110S.gb/4177058‑4177124
                                                                                                                                             
CGCTGCTGATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATT                                                                        <  1:3443296/71‑1
 GCTGCTGATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATTG                                                                       <  1:1672669/71‑1
 GCTGCTGATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATTG                                                                       <  1:4651484/71‑1
 GCTGCTGATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATTG                                                                       <  1:4060193/71‑1
  CTGCTGATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATTG                                                                       <  1:332444/70‑1
       GATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATAT                                                                         >  1:4868335/1‑63
       GATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATAT                                                                         >  1:1872404/1‑63
         TTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATA                                                                          >  1:3200424/1‑60
          TAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATTGA                                                                      >  1:2573693/1‑63
          TAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATTGA                                                                      >  1:1693747/1‑63
           AACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATA                                                                          >  1:2511542/1‑58
                 GGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATTG                                                                       <  1:4255915/55‑1
                      CGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATT                                                                        <  1:3551637/49‑1
                        ATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATT                                                                        <  1:522902/47‑1
                                                TGCAATCAATCGCTGACTATATTGATTGCACGATCTCATCGGTGTTTCCGCGATCGATACCGGC                               <  1:1907020/64‑1
                                                   AATCAATCGCTGACTATATTGATTGCACGATCTCATCGGTGT                                                  <  1:4763990/42‑1
                                                     TCAATCGCTGACTATATTGATTGCACGATCTCATCGGTGTTT                                                >  1:1744201/1‑42
                                                     TCAATCGCTGACTATATTGATTGCACGATCTCATCGGTGTTT                                                >  1:4835821/1‑42
                                                                    ATTGATTGCACGATCTCATCGGTGTTTCCGCGATC                                        <  1:2712617/35‑1
                                                                    ATTGATTGCACGATCTCATCGGTGTTTCCGCGATC                                        <  1:1472176/35‑1
                                                                     TTGATTGCACGATCTCATCGGTGTTTCCGCGATCGATACCGGCGTTAATCAGCAGCGCACTGAAT         <  1:3344426/65‑1
                                                                     TTGATTGCACGATCTCATCGGTGTTTCCGCGATCGATACCGGCGTTAATCAGCAGCGCACTG            <  1:4354516/62‑1
                                                                      TGATTGCACGATCTCATCGGTGTTTCCGCGATCGATACCGGCGTTAATCAGCAGCGCACTGAATACCTGGG  >  1:672009/1‑71
                                                                      TGATTGCACGATCTCATCGGTGTTTCCGCGATCGA                                      >  1:3590676/1‑35
                                                                                                                                             
CGCTGCTGATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATTGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/4177109‑4177036
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑attgatTGCACGATCTCATCGGTGTTTCCGCGATCGATACCGGCGTTAATCAGCAGCGCACTGAATACCTGGG  >  W3110S.gb/4177058‑4177124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.