| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 23,942 | G→A | 14.3% | G518S (GGC→AGC) | ileS → | isoleucyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 23,942 | 0 | G | A | 14.3% | 266.6 / 30.4 | 98 | G518S (GGC→AGC) | ileS | isoleucyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (49/35); new base A (9/5); total (58/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
GTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTA > W3110S.gb/23876‑24008 | gTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGcga > 1:1805303/1‑71 (MQ=255)gTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga > 1:1563777/1‑71 (MQ=255)gTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga > 1:3888577/1‑71 (MQ=255) ggCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcgac > 1:1442315/1‑70 (MQ=255) gCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGcgacga < 1:4867030/71‑1 (MQ=255) gCAAAACGCGTTGAAGTCGATAGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGcgacga < 1:2151616/71‑1 (MQ=255) aaaCGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGc > 1:2292051/1‑71 (MQ=255) aaaCGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGc > 1:3317682/1‑71 (MQ=255) aaaCGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGc > 1:1846126/1‑71 (MQ=255) aaaCGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGc > 1:788757/1‑71 (MQ=255) aaCGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCt > 1:131815/1‑71 (MQ=255) aaCGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCt > 1:137861/1‑71 (MQ=255) aaCGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCt > 1:329628/1‑71 (MQ=255) aaCGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCt < 1:1840098/71‑1 (MQ=255) aaCGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCt > 1:4149519/1‑71 (MQ=255) aaCGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCt > 1:491541/1‑71 (MQ=255) aaCGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCt > 1:2178063/1‑71 (MQ=255) aaCGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCt > 1:1035070/1‑71 (MQ=255) aCGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTg < 1:3458634/71‑1 (MQ=255) aCGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTg < 1:3549555/71‑1 (MQ=255) cgcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGa > 1:4547125/1‑71 (MQ=255) cgcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGa > 1:3157804/1‑71 (MQ=255) cgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATc < 1:2418432/71‑1 (MQ=255) cgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATc < 1:3503339/71‑1 (MQ=255) gTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGaa < 1:3414499/63‑1 (MQ=255) gTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGaa < 1:903906/63‑1 (MQ=255) tGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAg > 1:1480667/1‑70 (MQ=255) gAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTa > 1:2203733/1‑71 (MQ=255) gAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTa > 1:1490563/1‑71 (MQ=255) gAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTa > 1:2483623/1‑71 (MQ=255) aaGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTAc < 1:2170648/71‑1 (MQ=255) aaGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTAc < 1:2112571/71‑1 (MQ=255) aaGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTAc < 1:2927556/71‑1 (MQ=255) cGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTg > 1:5019523/1‑70 (MQ=255) cGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTg > 1:4579355/1‑70 (MQ=255) cGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTg > 1:2684413/1‑70 (MQ=255) gATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGa < 1:3335234/70‑1 (MQ=255) gATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGa < 1:1508907/70‑1 (MQ=255) gATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGa < 1:3590463/70‑1 (MQ=255) gATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGa > 1:2718902/1‑70 (MQ=255) gCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAg > 1:3947015/1‑51 (MQ=255) cATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGc < 1:627672/71‑1 (MQ=255) cATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTAAGTGAAAGTGc < 1:5086727/71‑1 (MQ=255) cATCCAGGCGGGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGc < 1:2619924/71‑1 (MQ=255) tCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGaaa > 1:429953/1‑65 (MQ=255) tCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGaaa > 1:3343599/1‑65 (MQ=255) ccAGGCGTGGTGGGATCTCGATGGGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCg > 1:2164724/1‑70 (MQ=255) ccAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGCCGAAGCTGATCAGTACGTGAAAGTGcc > 1:1400945/1‑69 (MQ=255) ccAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGa > 1:2537273/1‑51 (MQ=255) cAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGa > 1:2339102/1‑71 (MQ=255) cAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGa > 1:2206267/1‑71 (MQ=255) cAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGa > 1:2745426/1‑71 (MQ=255) ggCGTGGTGGGATCTCGATGCGAAAGAGATCCGCGGCGACGAAGCTGATCAGTACGTGa < 1:2219665/59‑1 (MQ=255) gCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGAt < 1:4195039/48‑1 (MQ=255) gCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGaca > 1:2412563/1‑70 (MQ=255) tggtggGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGaca < 1:2869282/67‑1 (MQ=255) tggtggGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGaca < 1:2387392/67‑1 (MQ=255) gtggGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGAcaca > 1:3323204/1‑67 (MQ=255) gtggGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTg < 1:1327256/70‑1 (MQ=255) tggGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATc < 1:2489295/43‑1 (MQ=255) tggGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGaa < 1:4574025/53‑1 (MQ=255) gtaTCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGAGACATTGGATg < 1:240290/69‑1 (MQ=255) ctcGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGa < 1:2169366/46‑1 (MQ=255) ctcGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAg > 1:4385957/1‑49 (MQ=255) ctcGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAg > 1:1671754/1‑49 (MQ=255) ctcGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATg > 1:1599772/1‑71 (MQ=255) ctcGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATg > 1:121019/1‑71 (MQ=255) gATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTa > 1:5078616/1‑38 (MQ=255) gATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTa > 1:3756936/1‑38 (MQ=255) gATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGa > 1:4848015/1‑62 (MQ=255) gATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATg > 1:2380827/1‑68 (MQ=255) gATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATg < 1:3109606/68‑1 (MQ=255) gATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATg < 1:3473663/68‑1 (MQ=255) gATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATg < 1:2774740/68‑1 (MQ=255) aTGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGttt < 1:278040/71‑1 (MQ=255) aTGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGttt < 1:2877759/71‑1 (MQ=255) tGCGAAAGAGATCCTCGGCGGCGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGttt > 1:2794098/1‑70 (MQ=255) gCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGaa > 1:704827/1‑41 (MQ=255) gCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGaa > 1:4810984/1‑41 (MQ=255) cGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGtt > 1:4258840/1‑67 (MQ=255) cGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGtt > 1:3841058/1‑67 (MQ=255) cGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGAc < 1:4746937/71‑1 (MQ=255) cGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGAc > 1:2271225/1‑71 (MQ=255) aaGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTg > 1:3207912/1‑41 (MQ=255) aaGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGaca > 1:2870924/1‑48 (MQ=255) aaGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGaca > 1:4725900/1‑48 (MQ=255) agagATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGCCTCCg > 1:1582625/1‑71 (MQ=255) gagaTCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCgg < 1:2211795/71‑1 (MQ=255) agaTCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGa > 1:1433529/1‑71 (MQ=255) aTCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTg < 1:2086089/48‑1 (MQ=255) aTCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTg < 1:1049203/48‑1 (MQ=255) aTCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATc < 1:1149607/71‑1 (MQ=255) tCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCt < 1:440072/71‑1 (MQ=255) ccTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTa > 1:2235304/1‑71 (MQ=255) ccTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTa > 1:2943094/1‑71 (MQ=255) cTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCg < 1:1171119/64‑1 (MQ=255) tCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATg < 1:4734012/48‑1 (MQ=255) cGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGAcaca > 1:685678/1‑40 (MQ=255) | GTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTA > W3110S.gb/23876‑24008 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |