| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,364,882 | G→A | 36.3% | V142V (GTG→GTA) | puuC → | gamma‑Glu‑gamma‑aminobutyraldehyde dehydrogenase, NAD(P)H‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,364,882 | 0 | G | A | 36.3% | 32.1 / 29.5 | 33 | V142V (GTG→GTA) | puuC | gamma‑Glu‑gamma‑aminobutyraldehyde dehydrogenase, NAD(P)H‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (10/11); new base A (7/5); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTGCCGC > W3110S.gb/1364814‑1364944 | gatATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGc > 1:4121441/1‑71 (MQ=255) atatTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGc < 1:473635/70‑1 (MQ=255) tCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGCAGCGATCGACAAAGTGTATGGCGAAGTAGCGa > 1:2393203/1‑68 (MQ=255) cccGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGaccac > 1:427632/1‑71 (MQ=255) cccGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac > 1:1051308/1‑71 (MQ=255) cccGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac > 1:1670491/1‑71 (MQ=255) cccGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGac > 1:2286909/1‑68 (MQ=255) ccGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGaccacc < 1:4306127/71‑1 (MQ=255) cgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCAt < 1:4972971/71‑1 (MQ=255) gcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATg < 1:2060095/71‑1 (MQ=255) cgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCAt > 1:2700548/1‑69 (MQ=255) cgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCAt > 1:3789910/1‑69 (MQ=255) gcCATTCGCTTGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCt < 1:217791/71‑1 (MQ=255) ccATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTg > 1:2700560/1‑71 (MQ=255) tCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCga > 1:3861199/1‑71 (MQ=255) tCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCg > 1:2208657/1‑70 (MQ=255) ggTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCGATGATc > 1:4309915/1‑71 (MQ=255) gTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATc < 1:4953203/70‑1 (MQ=255) aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTg > 1:4401643/1‑35 (MQ=255) aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATc > 1:2366483/1‑68 (MQ=255) gCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCGATGATcgtgcg < 1:44108/71‑1 (MQ=255) gCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCGATGATcgtgcg < 1:3498508/71‑1 (MQ=255) gAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATcgtgcgtg < 1:5058249/70‑1 (MQ=255) aGCGATCGACAAAGTGTATGGCGAAGTGGCGaccacc < 1:625063/37‑1 (MQ=255) gATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCg < 1:1354671/70‑1 (MQ=255) aTCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCGATGATcgtgcgtg < 1:1567117/64‑1 (MQ=255) tCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTc < 1:2617289/71‑1 (MQ=255) cAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCg < 1:371916/46‑1 (MQ=255) cAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCg < 1:1534073/46‑1 (MQ=255) aaaGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCg > 1:1686297/1‑70 (MQ=255) aaaGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGt > 1:1577582/1‑71 (MQ=255) aTGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTgcc > 1:3468200/1‑71 (MQ=255) aTGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTgc < 1:4838236/70‑1 (MQ=255) ggCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTgccgc > 1:99043/1‑71 (MQ=255) ggCGACCACCAGTAGCCATGAGCTGGCGATGATcg > 1:1138459/1‑35 (MQ=255) | GATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTGCCGC > W3110S.gb/1364814‑1364944 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |