| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,389,366 | A→G | 46.1% | L311L (TTA→TTG) | tyrR → | DNA‑binding transcriptional dual regulator, tyrosine‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,389,366 | 0 | A | G | 46.1% | 11.4 / 108.6 | 76 | L311L (TTA→TTG) | tyrR | DNA‑binding transcriptional dual regulator, tyrosine‑binding |
| Reads supporting (aligned to +/- strand): ref base A (18/23); new base G (22/13); total (40/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGCGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGT > W3110S.gb/1389299‑1389431 | aGGCGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTg < 1:46778/71‑1 (MQ=255) cGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGt < 1:1056494/71‑1 (MQ=255) cGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGt < 1:232284/71‑1 (MQ=255) cGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGt > 1:3276450/1‑71 (MQ=255) gAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGtt > 1:4737926/1‑71 (MQ=255) gAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGtt > 1:3311409/1‑71 (MQ=255) gAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGtt > 1:1722107/1‑71 (MQ=255) aaCGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGttt > 1:3081933/1‑71 (MQ=255) aaCGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGttt > 1:1762269/1‑71 (MQ=255) ggtTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTa < 1:1853225/70‑1 (MQ=255) ggtTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTa < 1:550610/70‑1 (MQ=255) tGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGc < 1:3962540/71‑1 (MQ=255) gCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTaa > 1:1129293/1‑63 (MQ=255) tGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTc > 1:4293712/1‑71 (MQ=255) tGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTc > 1:1408094/1‑71 (MQ=255) tGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTc > 1:1727621/1‑71 (MQ=255) ggATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTcc > 1:3551385/1‑71 (MQ=255) ggATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAat < 1:4881245/58‑1 (MQ=255) gATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAat < 1:4997832/57‑1 (MQ=255) gATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTcc < 1:434395/70‑1 (MQ=255) tGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTc > 1:3343472/1‑71 (MQ=255) gAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatgatg > 1:1465533/1‑58 (MQ=255) gAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatgatg > 1:3498098/1‑58 (MQ=255) gAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatgatg > 1:2545562/1‑58 (MQ=255) aaaTAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCgg < 1:1406829/71‑1 (MQ=255) aaTAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCgg < 1:182659/70‑1 (MQ=255) aaTAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTcc < 1:2396065/47‑1 (MQ=255) aaTAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCgg < 1:1570647/70‑1 (MQ=255) ggAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCg < 1:504418/71‑1 (MQ=255) ggAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCg < 1:4889103/71‑1 (MQ=255) ggAAATGTCACCACGGATGCAGGCGAAATTACTGCCTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCg < 1:2907910/71‑1 (MQ=255) tGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCg < 1:139120/66‑1 (MQ=255) tGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATAGCACTTTCCGTCGGGTTGGCg < 1:1273983/66‑1 (MQ=255) gTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTcc > 1:848967/1‑53 (MQ=255) gTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatga < 1:2956182/42‑1 (MQ=255) gTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTACCGt > 1:1063636/1‑55 (MQ=255) tCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAat < 1:2053734/39‑1 (MQ=255) accacGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt > 1:1815718/1‑70 (MQ=255) accacGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt > 1:161748/1‑70 (MQ=255) accacGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt > 1:1914428/1‑70 (MQ=255) accacGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATg < 1:4066727/71‑1 (MQ=255) accacGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATg < 1:3461156/71‑1 (MQ=255) ccacGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGa < 1:4339444/71‑1 (MQ=255) cacGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAg > 1:1979938/1‑71 (MQ=255) cacGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAg > 1:3443480/1‑71 (MQ=255) cGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGt > 1:1539090/1‑71 (MQ=255) cGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGt > 1:1449975/1‑71 (MQ=255) cGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGt > 1:4915470/1‑71 (MQ=255) cGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGt > 1:759204/1‑71 (MQ=255) cGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGt > 1:912997/1‑71 (MQ=255) tGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt < 1:1031206/62‑1 (MQ=255) tGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt < 1:3203194/62‑1 (MQ=255) tGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCAt < 1:320081/71‑1 (MQ=255) gCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATg > 1:458212/1‑71 (MQ=255) cAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGa > 1:111140/1‑62 (MQ=255) cAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATg > 1:3988663/1‑70 (MQ=255) cAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGt > 1:3817458/1‑71 (MQ=255) cAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATg > 1:2588146/1‑70 (MQ=255) aGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTc < 1:2099761/71‑1 (MQ=255) gCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCa > 1:790890/1‑56 (MQ=255) gCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCg < 1:1982797/70‑1 (MQ=255) gCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCg < 1:3982674/70‑1 (MQ=255) gCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGAc < 1:367341/54‑1 (MQ=255) cGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGa > 1:3696159/1‑70 (MQ=255) cGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAt > 1:2083286/1‑71 (MQ=255) gAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAt > 1:1553017/1‑70 (MQ=255) gAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAt > 1:2905639/1‑70 (MQ=255) gAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAt > 1:4293346/1‑70 (MQ=255) aaaTTACTGCGTTTCCTTAATGATGGCACTTTCCGt < 1:83990/36‑1 (MQ=255) aaaTTACTGCGTTTCCTTAATGATGGCACTTTCCGt < 1:3298469/36‑1 (MQ=255) aaaTTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt < 1:613691/54‑1 (MQ=255) aaaTTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGTATGTCGAtgt < 1:1524809/71‑1 (MQ=255) aaaTTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAtgt < 1:4168020/71‑1 (MQ=255) aaTTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAtg > 1:2747831/1‑69 (MQ=255) aTTACTGCGTTTCCTTAATGATGGCACTTTCCGTCg > 1:2647319/1‑36 (MQ=255) ttACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGc < 1:2233335/58‑1 (MQ=255) | AGGCGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGT > W3110S.gb/1389299‑1389431 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |