| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,393,837 | C→T | 40.4% | R45* (CGA→TGA) | ycjZ → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,393,837 | 0 | C | T | 40.4% | 31.4 / 53.2 | 47 | R45* (CGA→TGA) | ycjZ | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (11/17); new base T (7/12); total (18/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
ACTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCA > W3110S.gb/1393768‑1393901 | aCTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACg < 1:3295546/71‑1 (MQ=255) cTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAAc < 1:3308528/69‑1 (MQ=255) cTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGa > 1:1747908/1‑71 (MQ=255) cTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGa > 1:173445/1‑71 (MQ=255) gTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGa < 1:1064567/68‑1 (MQ=255) gTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGa < 1:3371917/68‑1 (MQ=255) gTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGa < 1:1691320/68‑1 (MQ=255) tGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTgg < 1:2972430/71‑1 (MQ=255) agcagcCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTg < 1:455601/67‑1 (MQ=255) gcagcCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTgg < 1:2259229/67‑1 (MQ=255) gcagcCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTgg < 1:1074731/67‑1 (MQ=255) gcCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCg < 1:1552502/71‑1 (MQ=255) gcCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTgg < 1:1576373/64‑1 (MQ=255) gcCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTgg < 1:4507880/64‑1 (MQ=255) gcCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTgg < 1:2794445/64‑1 (MQ=255) cccGCCTGAGCATGGCGCGGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTgg < 1:2327515/63‑1 (MQ=255) cGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTggg > 1:752398/1‑62 (MQ=255) cGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCt > 1:2466502/1‑71 (MQ=255) tGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTgg < 1:1170873/57‑1 (MQ=255) tGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCCTATAGAAGAACGATTgg < 1:313014/57‑1 (MQ=255) aGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGa > 1:39202/1‑51 (MQ=255) cATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGAcgc > 1:1985/1‑71 (MQ=255) aTGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGAcgcg < 1:3915582/71‑1 (MQ=255) gcgcAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAAc < 1:4228241/71‑1 (MQ=255) aGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGAGGCTTCTGACGCGAACCa > 1:2169088/1‑65 (MQ=255) aGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCAcgcg > 1:724664/1‑69 (MQ=255) cTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGAt > 1:2790593/1‑42 (MQ=255) cTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGAt > 1:4757044/1‑42 (MQ=255) tAAGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCgt < 1:3590092/70‑1 (MQ=255) aGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCgttg < 1:1886085/70‑1 (MQ=255) aGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCgttg < 1:1405340/70‑1 (MQ=255) aGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCgttgt < 1:2139905/71‑1 (MQ=255) gCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCgttgtt > 1:2888922/1‑71 (MQ=255) cAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTcc < 1:1437955/71‑1 (MQ=255) aGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCa < 1:243325/71‑1 (MQ=255) aGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTcc > 1:4860990/1‑70 (MQ=255) aGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCgttg > 1:4124259/1‑62 (MQ=255) gTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGa < 1:3853891/71‑1 (MQ=255) cgtcgtATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGc > 1:4020279/1‑71 (MQ=255) cgtcgtATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGc < 1:2347462/71‑1 (MQ=255) tcgtATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTcc < 1:4697876/60‑1 (MQ=255) gtATAGAAGAACGATTGGGATTG‑GGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGc > 1:150248/1‑70 (MQ=255) tataGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCg < 1:5079377/67‑1 (MQ=255) tataGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCa < 1:3777728/47‑1 (MQ=255) tataGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCa < 1:2476401/47‑1 (MQ=255) taGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCggg > 1:2218324/1‑67 (MQ=255) gaagaaTGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCa > 1:3333591/1‑71 (MQ=255) gaagaaCGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCa > 1:4597321/1‑71 (MQ=255) | ACTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCA > W3110S.gb/1393768‑1393901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |