| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,413,523 | G→A | 37.8% | S47F (TCC→TTC) | ydaO ← | predicted C32 tRNA thiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,413,523 | 0 | G | A | 37.8% | 38.2 / 46.9 | 45 | S47F (TCC→TTC) | ydaO | predicted C32 tRNA thiolase |
| Reads supporting (aligned to +/- strand): ref base G (15/13); new base A (10/7); total (25/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTAC > W3110S.gb/1413459‑1413592 | gattgGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAgg > 1:4239867/1‑70 (MQ=255)gattgGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAgg > 1:517119/1‑70 (MQ=255) ttgGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCaa < 1:892039/71‑1 (MQ=255) ttgGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCaa < 1:885723/71‑1 (MQ=255) cgcTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCat < 1:4881568/70‑1 (MQ=255) gcTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCatga > 1:988988/1‑71 (MQ=255) gcTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGAAGAGGCAAACCatga > 1:4845393/1‑71 (MQ=255) aTTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCct < 1:2700512/70‑1 (MQ=255) tGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCcttct > 1:1827795/1‑71 (MQ=255) tGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCcttct > 1:1953303/1‑71 (MQ=255) cAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCaa < 1:4016047/71‑1 (MQ=255) gAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCg < 1:371340/55‑1 (MQ=255) gAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCg < 1:2857228/55‑1 (MQ=255) aaTCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCa < 1:4184269/71‑1 (MQ=255) aaTCTCCAGCATGGTATAGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCa > 1:4757464/1‑71 (MQ=255) tctcCAGCATGGTATAGCTGTCTTTACCCCCGGAGAgg > 1:982040/1‑38 (MQ=255) tctcCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCata > 1:736274/1‑71 (MQ=255) tctcCAGCATGGTATAGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGTTCGCCTTCTTCAATCata > 1:1915337/1‑71 (MQ=255) tcCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCg < 1:2672865/50‑1 (MQ=255) tcCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCg < 1:1718322/50‑1 (MQ=255) tcCAGCATGGTATAGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATAtt > 1:3989526/1‑71 (MQ=255) cATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGt < 1:4136052/71‑1 (MQ=255) cATGGTATAGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCg < 1:1304680/45‑1 (MQ=255) gTATAGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGc < 1:2649123/71‑1 (MQ=255) gTATAGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGc < 1:4119267/71‑1 (MQ=255) tataGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGc > 1:1121193/1‑70 (MQ=255) tataGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGc < 1:3562744/39‑1 (MQ=255) taGCTGTCTTTACCCCCGGAGAGGCAAACCATAATGCg < 1:89605/38‑1 (MQ=255) gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAAt > 1:3363542/1‑51 (MQ=255) gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCa > 1:1117111/1‑67 (MQ=255) gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCa > 1:3338377/1‑67 (MQ=255) tGTCTTTACCCCAGGAGAGGCAAACCATGATGCGAt > 1:892057/1‑36 (MQ=255) gTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCat < 1:4838586/51‑1 (MQ=255) tttACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGc > 1:311132/1‑71 (MQ=255) tttACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGc > 1:1329488/1‑71 (MQ=255) ttACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGcc > 1:4412424/1‑71 (MQ=255) ttACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGcc > 1:1846085/1‑71 (MQ=255) cccccGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGaa > 1:4355774/1‑50 (MQ=255) ccccGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAg < 1:3550049/54‑1 (MQ=255) cccGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGt < 1:2161468/71‑1 (MQ=255) ccGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGc < 1:4526284/53‑1 (MQ=255) ccGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCa > 1:1251722/1‑54 (MQ=255) cGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGaa > 1:2609099/1‑46 (MQ=255) cGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGaa > 1:366397/1‑46 (MQ=255) cGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTa > 1:2896542/1‑71 (MQ=255) ggAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCaa > 1:3510449/1‑53 (MQ=255) gagaGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTac > 1:1956180/1‑70 (MQ=255) | GATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTAC > W3110S.gb/1413459‑1413592 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |