| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,458,727 | A→G | 25.0% | E195G (GAA→GGA) | paaE → | predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,458,727 | 0 | A | G | 25.0% | 76.0 / 26.9 | 44 | E195G (GAA→GGA) | paaE | predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation |
| Reads supporting (aligned to +/- strand): ref base A (13/20); new base G (5/6); total (18/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTG > W3110S.gb/1458660‑1458790 | cAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGgaa < 1:2348103/70‑1 (MQ=255)cAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGaaa < 1:2016214/70‑1 (MQ=255) gttgttGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAAt < 1:3448337/71‑1 (MQ=255) gtgCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGt > 1:3841666/1‑71 (MQ=255) cATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGGAAAATTACAGTCa < 1:4573103/70‑1 (MQ=255) cATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGGAAAATTACAGTCa < 1:3661342/70‑1 (MQ=255) cATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGGAAAATTACAGTCAc < 1:1928955/71‑1 (MQ=255) aTTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACt > 1:3708626/1‑71 (MQ=255) ttttCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACtt < 1:2131647/71‑1 (MQ=255) ttttCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACtt > 1:1514338/1‑71 (MQ=255) tttCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTg > 1:765892/1‑71 (MQ=255) ttCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCAGTTg < 1:3809035/70‑1 (MQ=255) ttCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTgg > 1:826650/1‑71 (MQ=255) gtcagGGAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCa < 1:1530100/62‑1 (MQ=255) gtcagGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGGAAAATTACAGTc < 1:1955431/61‑1 (MQ=255) gtcagGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGGAAAATTACAGTc < 1:2728193/61‑1 (MQ=255) gtcagGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGGAAAATTACAGTCAc < 1:4343176/63‑1 (MQ=255) gtcagGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCa < 1:3169858/62‑1 (MQ=255) cagGAACCCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGGAAAATTACAGTCAc > 1:842741/1‑61 (MQ=255) agGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTgg > 1:2887447/1‑64 (MQ=255) agGAAACCCTCGACAGAGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCg > 1:4608998/1‑71 (MQ=255) gAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCt < 1:3276422/71‑1 (MQ=255) aaCCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGGAAAATTACAGTCACTTGGGGCCTCGCTCa > 1:755142/1‑71 (MQ=255) ccTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGGAAAATTACAGTCACTTGGGGCCTCGCTCAt > 1:1478249/1‑69 (MQ=255) cTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGaaa < 1:3081959/40‑1 (MQ=255) tCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTg > 1:2074366/1‑54 (MQ=255) tCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTg > 1:4060959/1‑54 (MQ=255) cGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAAtt > 1:4178132/1‑71 (MQ=255) gACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAAttt > 1:3569332/1‑71 (MQ=255) cGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTa < 1:1137432/37‑1 (MQ=255) cGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTa < 1:29115/37‑1 (MQ=255) cGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTa < 1:4649164/37‑1 (MQ=255) cGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTa < 1:4655251/37‑1 (MQ=255) tGCTTCACGGGCGTATTGACGGTGGAAAATTACAGTCACTTGGGGCCTCGCTCATAAATTTTCGTCTTTat > 1:2068559/1‑71 (MQ=255) cTTCACGGGCGTATTGACGGTGGAAAATTACAGTCACTTGGGGCCTCg > 1:606061/1‑48 (MQ=255) cACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTatgatg < 1:5071966/70‑1 (MQ=255) cACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTatgat < 1:129888/69‑1 (MQ=255) cGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCatt < 1:1076760/71‑1 (MQ=255) tATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCAt < 1:1893315/42‑1 (MQ=255) tATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCAt < 1:738657/42‑1 (MQ=255) tATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCAt < 1:2497205/42‑1 (MQ=255) ttGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAg > 1:4858143/1‑62 (MQ=255) gACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTtg > 1:1309754/1‑71 (MQ=255) aCGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTtg < 1:2007147/70‑1 (MQ=255) aCGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTtg < 1:1027384/70‑1 (MQ=255) ggTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCatttattt < 1:1697420/67‑1 (MQ=255) ggTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCatttattt < 1:3692065/67‑1 (MQ=255) | CAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTG > W3110S.gb/1458660‑1458790 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |