| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,484,463 | T→C | 17.6% | I38V (ATC→GTC) | azoR ← | NADH‑azoreductase, FMN‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,484,463 | 0 | T | C | 17.6% | 155.9 / 28.5 | 68 | I38V (ATC→GTC) | azoR | NADH‑azoreductase, FMN‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (24/32); new base C (7/5); total (31/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01 | |||||||||||
CGCAGAGCGCCAACCAGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTGAGAG > W3110S.gb/1484396‑1484530 | cGCAGAGCGCCAACCAGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGACTtc < 1:497391/71‑1 (MQ=255) agagCGCCAACCAGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTtcatc < 1:1571332/71‑1 (MQ=255) gcgcCAACCAGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGc > 1:2130016/1‑71 (MQ=255) cgcCAACCAGTTCGCCATCCAGTATCGGAATCGGATTTGCAGCCAGGGCGCGAACGGTGATTtca < 1:1209987/65‑1 (MQ=255) aaCCAGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGt > 1:3813012/1‑71 (MQ=255) aCCAGTACGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTg > 1:659276/1‑71 (MQ=255) aGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTCCATCAGCGGAg > 1:3670702/1‑66 (MQ=255) cGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGTAGTGCTTTTcg > 1:1206264/1‑71 (MQ=255) cATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGATTGCTTTTCgcgc < 1:3076178/71‑1 (MQ=255) cATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCgcgc < 1:4537910/71‑1 (MQ=255) aTCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCgcgc > 1:2531844/1‑70 (MQ=255) aTCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCgcgc > 1:4666710/1‑70 (MQ=255) aTCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGcc < 1:4553904/71‑1 (MQ=255) tCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTtc < 1:1298128/47‑1 (MQ=255) tCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTtc < 1:5050564/47‑1 (MQ=255) tCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTtc < 1:1081587/47‑1 (MQ=255) tCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTtc < 1:4500614/47‑1 (MQ=255) tCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCg < 1:4152135/54‑1 (MQ=255) cAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCAt < 1:3623675/70‑1 (MQ=255) cAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCAt < 1:4920643/70‑1 (MQ=255) aGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCAt < 1:3639173/69‑1 (MQ=255) gTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTg > 1:1814586/1‑70 (MQ=255) ccGGAATCGGATTTGCATCCAGGTCGCGAACGGTGATTTCATCAGCTGAGTGCTTTTCGCGCCATTGTTca < 1:2080547/71‑1 (MQ=255) ggAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAg > 1:937290/1‑48 (MQ=255) aaTCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTcaacaa < 1:2374575/71‑1 (MQ=255) ttCGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt < 1:3524716/62‑1 (MQ=255) aTCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTcaacaa > 1:1669568/1‑70 (MQ=255) aTCGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt < 1:5034998/63‑1 (MQ=255) aTCGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt < 1:4233563/63‑1 (MQ=255) tCGGATTTGCAGCCAGGTCGCGAACGGTGATTtca < 1:4430830/35‑1 (MQ=255) tCGGATTTGCAGCCAGGTCGCGAACGGTGATTtca < 1:3986063/35‑1 (MQ=255) tCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTc < 1:4454317/52‑1 (MQ=255) tCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTc < 1:4313505/52‑1 (MQ=255) tCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACaaa > 1:892011/1‑70 (MQ=255) ggATTTGCAGCCAGGTCGCGAACGGTGATTtcatc > 1:2250317/1‑35 (MQ=255) ggATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTcaacaa > 1:3339518/1‑67 (MQ=255) ggATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAaata < 1:4306187/71‑1 (MQ=255) ggATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAg > 1:4426774/1‑42 (MQ=255) ggATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAg > 1:514466/1‑42 (MQ=255) ggATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAg > 1:3335535/1‑42 (MQ=255) ggATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAg > 1:3951733/1‑42 (MQ=255) ggATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAg > 1:2468196/1‑42 (MQ=255) aTTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAaataat > 1:5083749/1‑71 (MQ=255) aTTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAaataa < 1:2199057/70‑1 (MQ=255) ttGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCg > 1:1200951/1‑71 (MQ=255) cAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGAc < 1:2809369/71‑1 (MQ=255) cAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGAc < 1:454979/71‑1 (MQ=255) cAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACt < 1:3835836/71‑1 (MQ=255) ggTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGa > 1:2157265/1‑71 (MQ=255) gTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTcaacaa < 1:3270872/53‑1 (MQ=255) gTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTc < 1:4413076/48‑1 (MQ=255) gTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTc < 1:3645720/48‑1 (MQ=255) cgcgAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACaaaa > 1:2174963/1‑53 (MQ=255) cgcgAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGAt > 1:1835376/1‑69 (MQ=255) gcgAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGAt > 1:4135979/1‑68 (MQ=255) gcgAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGAt > 1:4622991/1‑68 (MQ=255) gcgAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTa < 1:2504296/70‑1 (MQ=255) cgAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGa < 1:3567450/71‑1 (MQ=255) gAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTcaacaa < 1:2456360/48‑1 (MQ=255) cGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAaata < 1:5138676/49‑1 (MQ=255) cGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTga < 1:4066656/71‑1 (MQ=255) ggTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACt > 1:1922376/1‑68 (MQ=255) gTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACaa > 1:765457/1‑56 (MQ=255) gTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGAt > 1:4907910/1‑61 (MQ=255) tGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTa < 1:4657932/62‑1 (MQ=255) gATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTGagag > 1:3467090/1‑70 (MQ=255) gATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTGagag > 1:3461508/1‑70 (MQ=255) gATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTGagag > 1:3346875/1‑70 (MQ=255) | CGCAGAGCGCCAACCAGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTGAGAG > W3110S.gb/1484396‑1484530 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |