| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,516,711 | C→T | 42.3% | A79V (GCG→GTG) | ydcV → | predicted spermidine/putrescine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,516,711 | 0 | C | T | 42.3% | 11.0 / 28.7 | 26 | A79V (GCG→GTG) | ydcV | predicted spermidine/putrescine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (8/7); new base T (7/4); total (15/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTC > W3110S.gb/1516643‑1516780 | acaGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCTACATTAATTgcgc > 1:2210479/1‑71 (MQ=255)acaGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTgcgc > 1:1392904/1‑71 (MQ=255)acaGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTgcgc > 1:4058561/1‑71 (MQ=255) gCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTgg < 1:1402997/71‑1 (MQ=255) gCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGAGGCGATGGCGACATTAATTGCGCTgg > 1:178759/1‑71 (MQ=255) gTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGgtgt > 1:4863634/1‑69 (MQ=255) tCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAgg > 1:4479019/1‑66 (MQ=255) cTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCt > 1:5063835/1‑71 (MQ=255) ttGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTg > 1:2435725/1‑71 (MQ=255) ttGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTg > 1:3310492/1‑71 (MQ=255) gATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCt < 1:4659870/68‑1 (MQ=255) gATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTgg < 1:1222500/70‑1 (MQ=255) gATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCt < 1:1277893/68‑1 (MQ=255) gTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGc < 1:2740013/71‑1 (MQ=255) acacTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTGGCAGCTGCcg > 1:3882906/1‑70 (MQ=255) tGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGtt < 1:2356972/46‑1 (MQ=255) gTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGtt < 1:214737/45‑1 (MQ=255) aCTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTGGCAg < 1:4769169/56‑1 (MQ=255) ttAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTgg < 1:2986351/51‑1 (MQ=255) aaaGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGa > 1:2233618/1‑43 (MQ=255) aaaGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGcgac > 1:57296/1‑70 (MQ=255) aaaGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGcgat > 1:1083033/1‑69 (MQ=255) aaaGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGcgac < 1:3861170/70‑1 (MQ=255) aaaGTGGCGGCGCTGGCGACAGTAATTGTGCTGGTGTTAGGGa > 1:4898965/1‑43 (MQ=38) gCGACATT‑ATTGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCaaa < 1:472965/71‑1 (MQ=255) ttAATTGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGc > 1:3383099/1‑71 (MQ=255) aaTTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCg > 1:2045400/1‑59 (MQ=255) aaTTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCg > 1:841730/1‑59 (MQ=255) aaTTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCa > 1:784590/1‑71 (MQ=255) gcgcTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTc > 1:1044170/1‑71 (MQ=255) | ACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTC > W3110S.gb/1516643‑1516780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |