| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,516,927 | A→G | 25.9% | N151S (AAT→AGT) | ydcV → | predicted spermidine/putrescine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,516,927 | 0 | A | G | 25.9% | 89.5 / 36.5 | 54 | N151S (AAT→AGT) | ydcV | predicted spermidine/putrescine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (21/19); new base G (5/9); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
TGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGC > W3110S.gb/1516861‑1516997 | tGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGt > 1:3218368/1‑70 (MQ=255)tGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGt > 1:2245328/1‑70 (MQ=255) gAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTc < 1:3698003/69‑1 (MQ=255) gAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTc < 1:686296/69‑1 (MQ=255) gAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTc < 1:580999/69‑1 (MQ=255) gAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTc < 1:2977002/69‑1 (MQ=255) gAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTc < 1:1625455/69‑1 (MQ=255) gAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTc < 1:4981987/69‑1 (MQ=255) tttttCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGttt < 1:1763058/71‑1 (MQ=255) ttttCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGtttt > 1:2787328/1‑71 (MQ=255) tttCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCa > 1:2041733/1‑59 (MQ=255) ccATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTcgccgc < 1:762953/71‑1 (MQ=255) ccATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTcgccgc < 1:1331751/71‑1 (MQ=255) ccATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTcgccgc < 1:416039/71‑1 (MQ=255) tCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCAcc > 1:117850/1‑71 (MQ=255) gTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTCGCCGCAcctc > 1:3989798/1‑71 (MQ=255) gTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTCGCCGCAcctc > 1:2965650/1‑71 (MQ=255) gTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTCGCCGCAcctc > 1:2670826/1‑71 (MQ=255) gTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAAt > 1:4047391/1‑45 (MQ=255) gTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAAt > 1:4594911/1‑45 (MQ=255) gTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCAcctc > 1:3881728/1‑71 (MQ=255) gTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCAcctc > 1:2023361/1‑71 (MQ=255) tcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTCGCCGCAcctcct < 1:1560831/65‑1 (MQ=255) tGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCa < 1:3386386/38‑1 (MQ=255) tGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCa < 1:373572/38‑1 (MQ=255) tGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCt < 1:4681202/69‑1 (MQ=255) tGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCt < 1:2227081/69‑1 (MQ=255) gCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGtttt > 1:1691950/1‑48 (MQ=255) gCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGtttt > 1:4439023/1‑48 (MQ=255) gCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGtttt > 1:1467856/1‑48 (MQ=255) cGACTTTTTGTGTAGTTGCGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGtt > 1:1005575/1‑71 (MQ=255) cTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGtttt > 1:2654484/1‑44 (MQ=255) cTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGtttt > 1:5125909/1‑44 (MQ=255) ttttGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGc > 1:556585/1‑71 (MQ=255) gtgtAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTc < 1:5123419/70‑1 (MQ=255) gtgtAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTc < 1:3270153/70‑1 (MQ=255) gtgtAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTc < 1:2288653/70‑1 (MQ=255) gtggtgTTTAACAGTGTCATCGCCCGTTTTCGCCGCAcctcc > 1:787397/1‑42 (MQ=255) gtggtgTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCt > 1:4271509/1‑71 (MQ=255) tggtgTTTAACAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCt < 1:1365560/70‑1 (MQ=255) gtgtTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCa < 1:2341762/60‑1 (MQ=255) gtgtTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATc < 1:61111/67‑1 (MQ=255) tgtTTAACAGTGTCATCGCCCGTTTTCGCCGCAcctcct < 1:2488786/39‑1 (MQ=255) tttAACAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTc < 1:615929/56‑1 (MQ=255) tttAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGa > 1:488698/1‑50 (MQ=255) tttAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGa > 1:5054329/1‑50 (MQ=255) tAACAATGTCATCGCCCGTTTTCGCCGCAcctcct > 1:157400/1‑35 (MQ=255) tAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAgg > 1:4924959/1‑50 (MQ=255) tAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGcc > 1:2956991/1‑70 (MQ=255) aaCAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTc < 1:4424689/53‑1 (MQ=255) aaCAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCa < 1:2853252/70‑1 (MQ=255) aCAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAAt < 1:3327118/71‑1 (MQ=255) aCAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAAt < 1:1608172/71‑1 (MQ=255) cAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATgg < 1:4443534/56‑1 (MQ=255) aTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAAtggc < 1:1466702/71‑1 (MQ=255) aTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAAtggc < 1:4746444/71‑1 (MQ=255) aTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAAt > 1:1262795/1‑68 (MQ=255) | TGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGC > W3110S.gb/1516861‑1516997 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |