| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,525,582 | A→G | 30.9% | T188A (ACC→GCC) | yncE → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,525,582 | 0 | A | G | 30.9% | 50.9 / 34.2 | 42 | T188A (ACC→GCC) | yncE | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (24/5); new base G (7/6); total (31/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.64e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
TGATTTGGGTCGTTGATGGCGGGAATATCAAACTGAAAACCGCCATCCAGAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTACACCACTAACGCTGACGGCGAATTGAT > W3110S.gb/1525514‑1525646 | tGATTTGGGTCGTTGATGGCGGGAATATCAAACTGAAAACCGCCATCCAGAACACCGGTAAAATGAGTGcc > 1:1894991/1‑71 (MQ=255) aTTTGGGTCGTTGATGGCGGGAATATCAAACTGAAAACCGCCATCCAGAACACCGGTAAAATGAGTACCgg > 1:3885487/1‑71 (MQ=255) ttGATGGCGGGAATATCAAACTGAAAACCGCCATCCAGAACACCGGTAAAATGAGTGCCGGTCTGGCGCTg < 1:942694/71‑1 (MQ=255) ggCGGGAATATCAAACTGAAAACCGCCATCCAGAACACCGGTAAAATAAGTGCCGGTCTGGCGCTGGATAg > 1:2169180/1‑71 (MQ=255) ggAATATCAAACTGAAAACCGCCATCCAGAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCg > 1:4422793/1‑69 (MQ=255) ggAATATCAAACTGAAAACCGCCATCCAGAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCg > 1:2650071/1‑69 (MQ=255) tCAAACTGAAAACCGCCATCCAGAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCGAAGGCa > 1:3257545/1‑69 (MQ=255) aaaCTGAAAACCGCCATCCAGAACACCGGTAAAATGAGTACCGGTc > 1:5116118/1‑46 (MQ=255) aaaCTGAAAACCGCCATCCAGAACACCGGTAAAATGAGTACCGGTCt > 1:3751874/1‑47 (MQ=255) tGAAAACCGCCATCCAGAACACCGGTAAAATGAGTACCGGTCTgg > 1:231115/1‑45 (MQ=255) tGAAAACCGCCATCCAGAACACCGGTAAAATGAGTACCGGTCTgg > 1:2604952/1‑45 (MQ=255) gAAAACCGCCATCCAGAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCtt > 1:3840078/1‑70 (MQ=255) gAAAACCGCCATCCAGAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCt > 1:4845602/1‑69 (MQ=255) aaaaCCGCCATCCAGAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCtt < 1:657281/69‑1 (MQ=255) aaaCCGCCATCCAGAACACCGGTAAAATGAGTGCCGGTCTGGCGCTGGATAGCGaa < 1:493928/56‑1 (MQ=255) aaaCCGCCATCCAGAACACCGGTAAAATGAGTGCCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCtt < 1:181953/68‑1 (MQ=255) aCCGCCATCCAGAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTacac > 1:2280968/1‑71 (MQ=255) cGCCATCCAGAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTAcacca > 1:4398835/1‑71 (MQ=255) cATCCAGAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTACACCACt < 1:4849124/70‑1 (MQ=255) tCCAGAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTACACCACt < 1:4966127/68‑1 (MQ=255) cAGAACACCGGTAAAATGAGTGCCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTacac < 1:875928/62‑1 (MQ=255) cAGAACACCGGTAAAATGAGTGCCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTACACCACTAACGc > 1:952461/1‑71 (MQ=255) cAGAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCGAAGGCaaa > 1:128725/1‑51 (MQ=255) cAGAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCGAAGGCaaa > 1:3834822/1‑51 (MQ=255) cAGAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCGAAGGCaaa > 1:3031102/1‑51 (MQ=255) cAGAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTACACCACTaa > 1:4340067/1‑68 (MQ=255) cAGAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTACACCACTAACGc > 1:279870/1‑71 (MQ=255) aGAACACCGGTAAAATGAGTGCCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTACACCACTAACGCt < 1:154435/71‑1 (MQ=255) gAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTACACCACTaa > 1:3564767/1‑66 (MQ=255) acacCGGTAAAATCAGTGCCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTACACCACTAACGCTGAc > 1:532445/1‑71 (MQ=255) acCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGc < 1:702926/36‑1 (MQ=255) ccGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTACACCACTAACGCTGACGGc > 1:1854215/1‑71 (MQ=255) ggTAAAATGAGTGCCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTACACCACTAACGCTGACGGCg > 1:806883/1‑70 (MQ=255) gTAAAATGAGTGCCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTaca > 1:94183/1‑51 (MQ=255) aaaaTGAGTGCCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTACACCACTAACGCTGACGGCGAAtt > 1:4271486/1‑71 (MQ=255) aTGAGTGCCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTACACCACTAACGCTGACGGCGAATTGAt < 1:2693024/71‑1 (MQ=255) gAGTACCGGTCTGGCGCTGGATAGCGAAGGCAAAc > 1:1710785/1‑35 (MQ=255) gAGTACCGGTCTGGCGCTGGATAGCGAAGGCAAACGt > 1:4785929/1‑37 (MQ=255) gAGTACCGGTCTGGCGCTGGATAGCGAAGGCAAACGt > 1:845856/1‑37 (MQ=255) aGTACCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTa > 1:4545428/1‑41 (MQ=255) aGTACCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTa > 1:2447960/1‑41 (MQ=255) tACCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTACACCACTAACGCTGACGGCg < 1:3171308/59‑1 (MQ=255) | TGATTTGGGTCGTTGATGGCGGGAATATCAAACTGAAAACCGCCATCCAGAACACCGGTAAAATGAGTACCGGTCTGGCGCTGGATAGCGAAGGCAAACGTCTTTACACCACTAACGCTGACGGCGAATTGAT > W3110S.gb/1525514‑1525646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |