| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,559,619 | A→G | 28.6% | L45S (TTA→TCA) | ddpF ← | D‑Ala‑D‑Ala transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,559,619 | 0 | A | G | 28.6% | 48.6 / 25.3 | 35 | L45S (TTA→TCA) | ddpF | D‑Ala‑D‑Ala transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (13/12); new base G (4/6); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAGCATACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAG > W3110S.gb/1559554‑1559689 | gcagcaTACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGtt > 1:1322850/1‑71 (MQ=255) gcaTACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTca > 1:900134/1‑71 (MQ=255) aTTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCAcggcg < 1:1331788/71‑1 (MQ=255) ttAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCAcggcg > 1:173293/1‑70 (MQ=255) tAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGa > 1:3730603/1‑71 (MQ=255) tAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGa > 1:4603117/1‑71 (MQ=255) aaaaGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGa > 1:4161123/1‑70 (MQ=255) aaaaGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGAt > 1:946336/1‑71 (MQ=255) aaaGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTcac < 1:3237602/60‑1 (MQ=255) tgtgCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTcac < 1:226711/55‑1 (MQ=255) tgCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAAt > 1:4307612/1‑71 (MQ=255) cGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATc > 1:4602496/1‑70 (MQ=255) cGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATc < 1:2417855/70‑1 (MQ=255) cGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATc < 1:1058167/70‑1 (MQ=255) tGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGTAAATc < 1:708815/64‑1 (MQ=255) tGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGTAAATc < 1:1997883/64‑1 (MQ=255) tGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCa < 1:4401949/71‑1 (MQ=255) ttttTCCGCAGCCTGACTCGCCGACGATCCCTAAGGt < 1:4758631/37‑1 (MQ=255) ttttGCCGCAGCCTGACTCGCCGACGATCCCTAAGGt < 1:845344/37‑1 (MQ=255) ttttGCCGCAGCCTGACTCGCCGACGATCCCTAAGGt < 1:3993366/37‑1 (MQ=255) ttttGCCGCAGCCTGACTCGCCGACGATCCCTAAGGt < 1:2993828/37‑1 (MQ=255) ttttGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTcac > 1:2803187/1‑42 (MQ=255) aGCCTTACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATg > 1:1424545/1‑70 (MQ=255) aGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGa < 1:2591952/71‑1 (MQ=255) aGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGa < 1:2161548/71‑1 (MQ=255) gCCTGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGaa < 1:3001824/71‑1 (MQ=255) gCCTGACTCGCCGACGATCCATGAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGaa < 1:3490916/71‑1 (MQ=255) cTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGt > 1:4237356/1‑40 (MQ=255) cTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGt > 1:51809/1‑40 (MQ=255) gCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCg < 1:921013/71‑1 (MQ=255) gCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCg > 1:4131194/1‑71 (MQ=255) gCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCg > 1:3232587/1‑71 (MQ=255) gacgaTCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAAc < 1:1821480/60‑1 (MQ=255) cccTAAGGTTTCACCACTGCGGATCTGTAAATCaa > 1:2397280/1‑35 (MQ=255) cccTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGt > 1:2122027/1‑66 (MQ=255) aaGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCaa > 1:3943476/1‑70 (MQ=255) aaGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAg > 1:632189/1‑71 (MQ=255) aaGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAg > 1:4307298/1‑71 (MQ=255) | GCAGCATACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAG > W3110S.gb/1559554‑1559689 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |