| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,584,624 | A→G | 35.4% | intergenic (‑386/+16) | ydeN ← / ← ydeO | conserved hypothetical protein/predicted DNA‑binding transcriptional acfivator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,584,624 | 0 | A | G | 35.4% | 61.9 / 67.0 | 65 | intergenic (‑386/+16) | ydeN/ydeO | conserved hypothetical protein/predicted DNA‑binding transcriptional acfivator |
| Reads supporting (aligned to +/- strand): ref base A (15/27); new base G (15/8); total (30/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.66e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTAAAAACAACGCGGGGCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGA > W3110S.gb/1584558‑1584694 | cTAAAAACAACGCGGGGCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCg < 1:4096672/71‑1 (MQ=255) aaaaCAACGCGGGGCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGt > 1:3821848/1‑69 (MQ=255) acaacGCGGGGCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCa < 1:3205812/71‑1 (MQ=255) acaacGCGGGGCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGc > 1:934256/1‑70 (MQ=255) gcgGGGCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAaataa < 1:2078093/71‑1 (MQ=255) gcgGGGCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAaataa < 1:2067006/71‑1 (MQ=255) gggCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCa < 1:996397/71‑1 (MQ=255) gggCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCa < 1:3107523/71‑1 (MQ=255) gggCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCa < 1:2724486/71‑1 (MQ=255) gggCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCa < 1:2109246/71‑1 (MQ=255) gCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCaaa > 1:1100475/1‑71 (MQ=255) gCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCaaa > 1:3752336/1‑71 (MQ=255) aGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCaaa > 1:4727082/1‑69 (MQ=255) gAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGc > 1:589443/1‑70 (MQ=255) aaTGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTa < 1:180294/71‑1 (MQ=255) gCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAg < 1:1488871/70‑1 (MQ=255) gCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGATAAAg < 1:4675408/70‑1 (MQ=255) gCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAg > 1:908451/1‑70 (MQ=255) cTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCaaa > 1:818128/1‑60 (MQ=255) ccccATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCAtt > 1:2371738/1‑70 (MQ=255) ccccATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTc < 1:4066595/71‑1 (MQ=255) ccATTTAATTCTTACGCAGCGTGTGTGGTTGacta > 1:3806061/1‑35 (MQ=255) ccATTTAATTCTTACGCAGCGTGTGTGGTTGacta > 1:4724212/1‑35 (MQ=255) ccATTTAATTCTTACGCAGCGTGTGTGGTTGacta > 1:693805/1‑35 (MQ=255) ccATTTAATTCTTACGCAGCGTGTGTGGTTGacta > 1:779267/1‑35 (MQ=255) cATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAAt > 1:260394/1‑55 (MQ=255) tttAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTaaa > 1:3858305/1‑60 (MQ=255) tttAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAg < 1:4199898/61‑1 (MQ=255) ttAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTa > 1:5031203/1‑57 (MQ=255) ttAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAaat > 1:4977905/1‑44 (MQ=255) tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGt > 1:3052479/1‑35 (MQ=255) tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgtgt > 1:195975/1‑71 (MQ=255) tAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCa > 1:3457822/1‑65 (MQ=255) tAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCa > 1:2283760/1‑65 (MQ=255) ttCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCaaa > 1:4877395/1‑47 (MQ=255) ttCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCa < 1:4846758/37‑1 (MQ=255) ttCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGc < 1:770585/71‑1 (MQ=255) cTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGccc > 1:280703/1‑71 (MQ=255) cTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGccc > 1:4217165/1‑71 (MQ=255) cTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCAt > 1:272911/1‑61 (MQ=255) aCGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCg < 1:1796180/69‑1 (MQ=255) aCGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCg < 1:921430/69‑1 (MQ=255) aCGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGt < 1:4740966/70‑1 (MQ=255) gCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTc < 1:3281438/54‑1 (MQ=255) gCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTc < 1:4472344/54‑1 (MQ=255) gCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTc < 1:95473/54‑1 (MQ=255) gCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTAtt < 1:4919703/71‑1 (MQ=255) aGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTc < 1:3313601/70‑1 (MQ=255) gCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCa > 1:1951147/1‑70 (MQ=255) tgtgtgGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAcc > 1:2965930/1‑71 (MQ=255) gtgtgGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCAt < 1:1272721/67‑1 (MQ=255) tgtgGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAg > 1:4932025/1‑41 (MQ=255) gtgGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCAt < 1:4515764/43‑1 (MQ=255) gtgGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCAt < 1:1420810/43‑1 (MQ=255) tgGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGc > 1:2074873/1‑54 (MQ=255) tgGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCgtg > 1:1918778/1‑71 (MQ=255) tgGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCgt > 1:3734144/1‑70 (MQ=255) gTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTa > 1:4500507/1‑57 (MQ=255) gTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTa > 1:3334855/1‑57 (MQ=255) gTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTa > 1:30420/1‑57 (MQ=255) gTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTAtt < 1:4490246/59‑1 (MQ=255) gTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTAtt < 1:3706219/59‑1 (MQ=255) gTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTAtt < 1:4205344/59‑1 (MQ=255) gTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTAtt < 1:3125426/59‑1 (MQ=255) gTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCgtgt < 1:1595410/70‑1 (MQ=255) ttGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCgtg < 1:1056920/68‑1 (MQ=255) ttGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCgtg < 1:1586980/68‑1 (MQ=255) ttGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCAt < 1:1803236/39‑1 (MQ=255) ttGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCAt < 1:3546566/39‑1 (MQ=255) actactCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACtt < 1:1594753/71‑1 (MQ=255) ctactCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACttt < 1:2508537/71‑1 (MQ=255) ctactCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACttt < 1:4425654/71‑1 (MQ=255) tactCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCg < 1:3143284/49‑1 (MQ=255) actCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGa > 1:2719467/1‑71 (MQ=255) actCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGa > 1:4689748/1‑71 (MQ=255) | CTAAAAACAACGCGGGGCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGA > W3110S.gb/1584558‑1584694 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |