Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,682,290 C→T 39.1% C340C (TGC→TGT ydgI → predicted arginine/ornithine antiporter transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,682,2900CT39.1% 49.9 / 70.5 64C340C (TGC→TGTydgIpredicted arginine/ornithine antiporter transporter
Reads supporting (aligned to +/- strand):  ref base C (10/29);  new base T (13/12);  total (23/41)
Fisher's exact test for biased strand distribution p-value = 3.77e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCA  >  W3110S.gb/1682221‑1682351
                                                                     |                                                             
cccGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGc                                                               >  1:1770022/1‑70 (MQ=255)
cccGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGc                                                               <  1:1826461/70‑1 (MQ=255)
    cgcATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGCTGACCAACATCTGCGTGCa                                                          <  1:3967785/71‑1 (MQ=255)
      cATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCaaa                                                        <  1:3112478/71‑1 (MQ=255)
      cATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCaaa                                                        <  1:1779269/71‑1 (MQ=255)
       aTTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAAt                                                       >  1:1234584/1‑71 (MQ=255)
          tttGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAAttt                                                     <  1:2489212/70‑1 (MQ=255)
           ttGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGTGTGCAAATTTGt                                                   >  1:4935404/1‑71 (MQ=255)
               gcgcCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTgg                                               <  1:390436/71‑1 (MQ=255)
                   cAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACt                                           <  1:3005071/71‑1 (MQ=255)
                    aGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGTGTGCAAATTTGTCTGGTACTc                                          <  1:3269414/71‑1 (MQ=255)
                    aGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGTGTGCAAATTTGTCTGGTACTc                                          <  1:632208/71‑1 (MQ=255)
                    aGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACt                                           >  1:107176/1‑70 (MQ=255)
                    aGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTc                                          <  1:378432/71‑1 (MQ=255)
                    aGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTc                                          <  1:2102809/71‑1 (MQ=255)
                     gAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCa                                         <  1:808460/71‑1 (MQ=255)
                     gAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCa                                         <  1:245665/71‑1 (MQ=255)
                       aCGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGTGTGCAAATTTGTCTGGTACTCATc                                       >  1:1571903/1‑71 (MQ=255)
                         gCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGTGTGCAAATTTg                                                    >  1:1787276/1‑56 (MQ=255)
                              aGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGTGTGCAAATTTGTCTGGTa                                             >  1:5139470/1‑58 (MQ=255)
                              aGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGTTACTCATCTGGCTGa                                >  1:2865900/1‑71 (MQ=255)
                               gCTGCGCCATCCGCCTCACTGTGGTTGACCATCATCTGCGTGCAAAttt                                                     <  1:1417594/49‑1 (MQ=255)
                               gCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGTGTGCAAAt                                                       >  1:4408315/1‑47 (MQ=255)
                                cTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAAt                                                       <  1:2649791/46‑1 (MQ=255)
                                cTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAAt                                                       <  1:1995674/46‑1 (MQ=255)
                                cTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTgg                                    <  1:2863731/65‑1 (MQ=255)
                                cTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTgg                                    <  1:1676891/65‑1 (MQ=255)
                                 tGCGCCATCCGCCTCACTTTGGTTGACCAACATCTGCGTGCAAAt                                                       <  1:1442060/45‑1 (MQ=255)
                                     ccATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTg                                 <  1:2289494/63‑1 (MQ=255)
                                      cATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTcc                        <  1:4984616/71‑1 (MQ=255)
                                        tctgCCTCACTGTGGTTGACCAACATCTGTGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCg                       <  1:4775874/67‑1 (MQ=255)
                                        tCCGCCTCACTGTGGTTGACCAACATCTGTGTGCAAATTTGTCt                                                 <  1:4233033/44‑1 (MQ=255)
                                        tCCGCCTCACTGTGGTTGACCAACATCTGTGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGa                      <  1:2925506/71‑1 (MQ=255)
                                        tCCGCCTCACTGTGGTTGACCAACATCTGCGTGCaaa                                                        <  1:4746557/37‑1 (MQ=255)
                                        tCCGCCTCACTGTGGTTGACCAACATCTGCGTGCaaa                                                        <  1:5039883/37‑1 (MQ=255)
                                         ccGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTgg                                               <  1:2493092/45‑1 (MQ=255)
                                          cGCCTCACTGTGGTTGACCAACATCTGTGTGCAAATTTGTCTgg                                               >  1:4940538/1‑44 (MQ=255)
                                          cGCCTCACTGTGGTTGACCAACATCTGTGTGCAAATTTGTCTg                                                >  1:507698/1‑43 (MQ=255)
                                           gCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTa                   <  1:1910133/71‑1 (MQ=255)
                                            ccTCACTGTGGTTGACCAACATCTGTGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCg                       <  1:3016053/66‑1 (MQ=255)
                                            ccTCACTGTGGTTGACCAACATCTGTGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCg                       <  1:395829/66‑1 (MQ=255)
                                              tCACTGTGGTTGACCAACATCTGTGTGCAAATTTGTCTGGTACTc                                          <  1:2747144/45‑1 (MQ=255)
                                              tCACTGTGGTTGACCAACATCTGTGTGCAAATTTGTCTGGTACTc                                          <  1:4390400/45‑1 (MQ=255)
                                               cACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTacaa                >  1:3829728/1‑70 (MQ=255)
                                                aCTGTGGTTGACCAACATCTGTTTGCAAATTTGTCTGGTACTCAt                                        >  1:3711666/1‑45 (MQ=255)
                                                aCTGTGGTTGACCAACATCTGTGTGCAAATTTGTCTGGTACTCAt                                        >  1:3834971/1‑45 (MQ=255)
                                                aCTGTGGTTGACCAACATCTGTGTGCAAATTTGTCTGGTACTCAt                                        >  1:2250075/1‑45 (MQ=255)
                                                aCTGTGGTTGACCAACATCTGTGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTacaaca              <  1:2191484/71‑1 (MQ=255)
                                                aCTGTGGTTGACCAACATCTGTGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTacaa                <  1:5140902/69‑1 (MQ=255)
                                                aCTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTacaaca              >  1:3987095/1‑71 (MQ=255)
                                                aCTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTacaaca              >  1:5139882/1‑71 (MQ=255)
                                                 cTGTGGTTGACCAACATCTGTGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAacac             <  1:2713655/71‑1 (MQ=255)
                                                   gtgGTTGACCAACATCTGTGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGt           >  1:1360497/1‑71 (MQ=255)
                                                    tgGTTGACCAACATCTGTGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGtt          >  1:1218056/1‑71 (MQ=255)
                                                    tgGTTGACCAACATCTGCGTGCAAATTTGTCTGGt                                              <  1:1839704/35‑1 (MQ=255)
                                                    tgGTTGACCAACATCTGCGTGCAAATTTGTCTGGt                                              >  1:838583/1‑35 (MQ=255)
                                                    tgGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGtt          >  1:2846486/1‑71 (MQ=255)
                                                    tgGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGtt          >  1:954995/1‑71 (MQ=255)
                                                         gACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTTGCTGACCGGTTCCGATTACAACACGTTGCTg      <  1:338477/70‑1 (MQ=255)
                                                         gACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTg      <  1:220940/70‑1 (MQ=255)
                                                         gACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGa     >  1:3087007/1‑71 (MQ=255)
                                                          aCCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGAc    <  1:326512/71‑1 (MQ=255)
                                                          aCCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGAc    <  1:1734275/71‑1 (MQ=255)
                                                           ccAACATCTGTGTGCAAATTTGTCTGGTACTCATCTGGCTGa                                >  1:1921918/1‑42 (MQ=255)
                                                            cAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCa  <  1:515494/71‑1 (MQ=255)
                                                                 tCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGAcc                              <  1:3029184/38‑1 (MQ=255)
                                                                     |                                                             
CCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCA  >  W3110S.gb/1682221‑1682351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: