| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,683,257 | G→A | 29.5% | D190N (GAT→AAT) | folM → | dihydrofolate reductase isozyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,683,257 | 0 | G | A | 29.5% | 63.4 / 32.3 | 44 | D190N (GAT→AAT) | folM | dihydrofolate reductase isozyme |
| Reads supporting (aligned to +/- strand): ref base G (15/16); new base A (5/8); total (20/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 | |||||||||||
CCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAG > W3110S.gb/1683189‑1683323 | cccGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgat < 1:3271642/71‑1 (MQ=255) gCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatgat > 1:1382298/1‑71 (MQ=255) gCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatgat > 1:2335466/1‑71 (MQ=255) gCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatgat < 1:4942226/71‑1 (MQ=255) gCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACataatgat < 1:1062652/71‑1 (MQ=255) gCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACataatga > 1:1118709/1‑70 (MQ=255) gCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCg < 1:2725494/71‑1 (MQ=255) gCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCg < 1:1504597/71‑1 (MQ=255) gCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCg < 1:535972/71‑1 (MQ=255) cACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATAATGATGCCGAatat < 1:1115231/71‑1 (MQ=255) ggAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatga > 1:4885046/1‑57 (MQ=255) aagtgaaagtgaATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGacaa < 1:3747253/71‑1 (MQ=255) agtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGc > 1:3438422/1‑51 (MQ=255) agtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGc > 1:1669992/1‑51 (MQ=255) agtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGc > 1:3800053/1‑51 (MQ=255) agtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGc > 1:2731055/1‑51 (MQ=255) tgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACataat < 1:2043664/44‑1 (MQ=255) tgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACataat < 1:697132/44‑1 (MQ=255) aaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAAGCGCTGa < 1:4553030/70‑1 (MQ=255) aaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATAGACAACAGGCGCTGa < 1:1853646/70‑1 (MQ=255) ttCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgat < 1:897371/40‑1 (MQ=255) cTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGacaa > 1:3242532/1‑56 (MQ=255) cTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGa > 1:30091/1‑53 (MQ=255) cgcCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACt < 1:3547233/70‑1 (MQ=255) aTCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtgatga < 1:3070648/71‑1 (MQ=255) tCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATc < 1:4503652/40‑1 (MQ=255) tCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATc < 1:3393965/40‑1 (MQ=255) tCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaa < 1:3008958/71‑1 (MQ=255) tCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaa < 1:412165/71‑1 (MQ=255) tCGCTGATCCTGTTTAATGAACATAATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaa < 1:3224990/71‑1 (MQ=255) tCGCTCATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaa < 1:517179/71‑1 (MQ=255) gCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGaca > 1:2882914/1‑42 (MQ=255) gCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGgcg > 1:96933/1‑49 (MQ=255) gCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGgcg > 1:1418060/1‑49 (MQ=255) gCTGATCCTGTTTAATGAACATAATGATGCCGAATATCGacaac > 1:2683589/1‑44 (MQ=255) gCTGATCCTGTTTAATGAACATAATGATGCCGAATATCGacaac > 1:908409/1‑44 (MQ=255) gCTGATCCTGTTTAATGAACATAATGATGCCGAATATCGacaac > 1:4763396/1‑44 (MQ=255) gCTGATCCTGTTTAATGAACATAATGATGCCGAATATCGacaac > 1:144703/1‑44 (MQ=255) gCTGATCCTGTTTAATGAACATAATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaaa < 1:962387/70‑1 (MQ=255) gCTGATCCTGTTTAATGAACATAATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaaa < 1:787681/70‑1 (MQ=255) ttAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGc > 1:1382647/1‑71 (MQ=255) aaTGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGc > 1:3281016/1‑69 (MQ=255) aTGAACATAATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACcgc > 1:5081862/1‑61 (MQ=255) tGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaa < 1:1638577/54‑1 (MQ=255) tGAACATAATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtg < 1:1790583/49‑1 (MQ=255) tGAACATAATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtg < 1:4109219/49‑1 (MQ=255) aaCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaaaa > 1:2382844/1‑54 (MQ=255) aaCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaaaa > 1:733256/1‑54 (MQ=255) aaCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaaaa > 1:4806384/1‑54 (MQ=255) aaCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCgcgc > 1:2469144/1‑60 (MQ=255) aaCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGagaa < 1:4756960/70‑1 (MQ=255) aCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGagaaag < 1:3303457/71‑1 (MQ=255) aCATAATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCTTGGCGagaaag < 1:4488386/71‑1 (MQ=255) aCATAATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGagaaag < 1:5113700/71‑1 (MQ=255) tgatgatgCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaaa < 1:1949486/49‑1 (MQ=255) | CCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAG > W3110S.gb/1683189‑1683323 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |