| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,691,217 | G→A | 27.3% | A310T (GCA→ACA) | manA → | mannose‑6‑phosphate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,691,217 | 0 | G | A | 27.3% | 93.9 / 36.2 | 55 | A310T (GCA→ACA) | manA | mannose‑6‑phosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base G (25/15); new base A (4/11); total (29/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.21e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATAAAGA > W3110S.gb/1691153‑1691284 | tGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTACAGaa > 1:2084860/1‑70 (MQ=255) ccAATGTGAAATTCGAAGCCAAACCGTCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTg < 1:1301932/71‑1 (MQ=255) cAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCAGGTGAAACAAGGTACAGAACTgg < 1:255568/71‑1 (MQ=255) aTGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTACAGAACTGGAc > 1:2930711/1‑71 (MQ=255) aTGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCAGTGAAACAAGGTGCAGAACTGGAc > 1:1651322/1‑71 (MQ=255) tgtgAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGaa > 1:2591147/1‑64 (MQ=255) tgtgAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGaa > 1:2243859/1‑64 (MQ=255) tgtgAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGaa > 1:2790716/1‑64 (MQ=255) tgAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTc > 1:5073054/1‑71 (MQ=255) tgAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTc > 1:3400828/1‑71 (MQ=255) tgAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTACAGAACTGGACTTc < 1:4542517/71‑1 (MQ=255) aaaTTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTACAGAACTGGACt > 1:2388244/1‑67 (MQ=255) aaaTTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTACAGAACTGGACTTccc < 1:2086748/71‑1 (MQ=255) aaTTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTccc > 1:5011433/1‑70 (MQ=255) aTTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAg < 1:3087023/56‑1 (MQ=255) ttCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAg < 1:2712783/55‑1 (MQ=255) cGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGAc > 1:5049426/1‑61 (MQ=255) cGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTc > 1:3842100/1‑71 (MQ=255) gCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTACAGAACTGGACTTCCCGATTCCAGt > 1:345335/1‑71 (MQ=255) aaaCCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTg > 1:3595446/1‑43 (MQ=255) cGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGgatgat > 1:1652420/1‑71 (MQ=255) gCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGAttt > 1:2184097/1‑71 (MQ=255) gCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGAttt > 1:1869670/1‑71 (MQ=255) gCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGCTGCAGAACTGGACTTCCCGATTCCAGTGGATGAttt > 1:2209284/1‑71 (MQ=255) ccAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCt > 1:2649536/1‑71 (MQ=255) cAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCtt < 1:2477422/71‑1 (MQ=255) cAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCtt < 1:2322298/71‑1 (MQ=255) cAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCtt < 1:30493/71‑1 (MQ=255) ttgttgACCCAGCCGGTGAAACAAGGTACAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTtctc < 1:2650364/71‑1 (MQ=255) gttgACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTcc > 1:4722835/1‑41 (MQ=255) cccAGCCGGTGAAACAAGGTACAGAACTGGACTTCCCGATTcc < 1:115680/43‑1 (MQ=255) cAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGa > 1:3721562/1‑71 (MQ=255) cGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCt > 1:1227222/1‑70 (MQ=255) cGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGAACt > 1:3226473/1‑70 (MQ=255) ggTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGAtt < 1:3008268/47‑1 (MQ=255) gTGAAACAAGGTACAGAACTGGACTTCCCGATTCCAGTGgat < 1:4737855/42‑1 (MQ=255) gTGAAACAAGGTACAGAACTGGACTTCCCGATTCCAGTGgat < 1:4039049/42‑1 (MQ=255) gTGAAACAAGGTACAGAACTGGACTTCCCGATTCCAGTGgat < 1:1198930/42‑1 (MQ=255) gTGAAACAAGGTACAGAACTGGACTTCCCGATTCCAGTGgat < 1:1055563/42‑1 (MQ=255) tGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGAtttt > 1:4254415/1‑47 (MQ=255) tGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGAtttt > 1:1904061/1‑47 (MQ=255) tGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCt < 1:1517126/67‑1 (MQ=255) tGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGt < 1:3528256/71‑1 (MQ=255) tGAAACAAGGTGCAGAACTGGACTTCACGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGt < 1:3478506/71‑1 (MQ=255) aaaCAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTg > 1:346540/1‑70 (MQ=255) aaaCAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGa > 1:3040398/1‑71 (MQ=255) aaCAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGAt < 1:266969/71‑1 (MQ=255) aaCAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGAt < 1:4724775/71‑1 (MQ=255) aCAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATa < 1:747009/71‑1 (MQ=255) aCAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATa < 1:1511321/71‑1 (MQ=255) aCAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATa < 1:3665251/71‑1 (MQ=255) aGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAg > 1:1349717/1‑63 (MQ=255) aGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATa > 1:1343713/1‑68 (MQ=255) aGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATa > 1:550980/1‑68 (MQ=255) ggTACAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCtt < 1:3839051/60‑1 (MQ=255) ggTACAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATAAAGa < 1:3434653/71‑1 (MQ=255) | TGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATAAAGA > W3110S.gb/1691153‑1691284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |