| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,700,325 | C→A | 36.1% | G190G (GGG→GGT) | malI ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,700,325 | 0 | C | A | 36.1% | 82.3 / 35.4 | 47 | G190G (GGG→GGT) | malI | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (19/11); new base A (5/12); total (24/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.55e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAG > W3110S.gb/1700262‑1700389 | aaaTTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGACCTCcca < 1:2161496/71‑1 (MQ=255) aTTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGTAACTTTGACCTCccagc < 1:1924763/71‑1 (MQ=255) aTTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGACCTCccagc > 1:4759244/1‑71 (MQ=255) tttAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCccagc > 1:124513/1‑69 (MQ=255) tttAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCccagc > 1:884424/1‑69 (MQ=255) ttAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGACCTCccagccag > 1:1617899/1‑71 (MQ=255) gTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCg > 1:2296360/1‑71 (MQ=255) gCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt > 1:821000/1‑70 (MQ=255) gCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt < 1:3239029/70‑1 (MQ=255) gCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt < 1:4660064/70‑1 (MQ=255) cacaATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTg < 1:2422154/70‑1 (MQ=255) cacaATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTg < 1:2687117/70‑1 (MQ=255) cacaATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTg < 1:4688207/70‑1 (MQ=255) cccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGc < 1:1692614/71‑1 (MQ=255) cccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGc < 1:1597361/71‑1 (MQ=255) cccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGACCTCCCAGCCAGGCg < 1:4380049/52‑1 (MQ=255) ccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGc < 1:1258472/70‑1 (MQ=255) gTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGaa > 1:357835/1‑66 (MQ=255) cTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGc > 1:2422345/1‑71 (MQ=255) cTGCACGGGTTAATGAGGAACTTTGACCTCCCAGCCAGGCGATCCGCTGa > 1:2157318/1‑50 (MQ=255) cACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTcc > 1:3878503/1‑71 (MQ=255) aCGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGa > 1:3864486/1‑46 (MQ=255) aCGGGTTAATGAGGAACTTTGCCATCCCAGCCAGGCGATCCGCTGa > 1:1631924/1‑46 (MQ=255) cGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGt > 1:3208363/1‑71 (MQ=255) gggTTAATGAGGAACTTTGACCTCCCAGCCAGGCGATCCGCt < 1:2693040/42‑1 (MQ=255) gggTTAATGAGGAACTTTGACCTCCCAGCCAGGCGATCCGCt < 1:269423/42‑1 (MQ=255) gggTTAATGAGGAACTTTGACCTCCCAGCCAGGCGATCCGCt < 1:1892133/42‑1 (MQ=255) tAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac > 1:2323163/1‑70 (MQ=255) tAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac > 1:2300354/1‑70 (MQ=255) ggAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATgag > 1:1624719/1‑51 (MQ=255) ggAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATgag > 1:3349239/1‑51 (MQ=255) ggAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATgag > 1:4260119/1‑51 (MQ=255) gAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACtgtg < 1:624815/70‑1 (MQ=255) gAACTTTGCACTCCCAGCCAGGCGATCCGCTGATGCCCATTGAGAATGAGATGCTCCGTCAACAACtgtg > 1:1693878/1‑70 (MQ=255) gAACTTTGACCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACtgtg > 1:243202/1‑70 (MQ=255) aaCTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCa < 1:2588976/71‑1 (MQ=255) aaCTTTGACCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCa < 1:1382039/71‑1 (MQ=255) aCTTTGCCCTCCCAGCCAGGCGATCCGCTGATGccc > 1:3706162/1‑36 (MQ=255) aCTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCa > 1:2224141/1‑37 (MQ=255) aCTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCa > 1:2324253/1‑37 (MQ=255) aCTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAg < 1:4476199/71‑1 (MQ=255) aCTTTGACCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAg < 1:2188019/71‑1 (MQ=255) aCTTTGACCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAg < 1:4853523/71‑1 (MQ=255) aCTTTGACCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAg < 1:2680291/71‑1 (MQ=255) cTTTGACCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTc < 1:4619502/57‑1 (MQ=255) cTTTGACCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTc < 1:348747/57‑1 (MQ=255) ttGACCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaa > 1:387060/1‑57 (MQ=255) | AAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAG > W3110S.gb/1700262‑1700389 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |