| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,717,162 | C→T | 5.8% | V148I (GTT→ATT) | pdxY ← | pyridoxal kinase 2/pyridoxine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,717,162 | 0 | C | T | 5.8% | 228.7 / 3.0 | 69 | V148I (GTT→ATT) | pdxY | pyridoxal kinase 2/pyridoxine kinase |
| Reads supporting (aligned to +/- strand): ref base C (32/33); new base T (2/2); total (34/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
AGTTCGCGCGCTGCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGC > W3110S.gb/1717092‑1717231 | aGTTCGCGCGCTGCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAAc > 1:4579390/1‑71 (MQ=255) cgcgcgCTGCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAAc > 1:2836740/1‑67 (MQ=255) cgcgcgCTGCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAAc > 1:397620/1‑67 (MQ=255) cgcgcgCTGCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAAc > 1:2259243/1‑67 (MQ=255) cgcgcgCTGCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAAc > 1:1961541/1‑67 (MQ=255) cgcgcgCTGCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAAc > 1:3763684/1‑67 (MQ=255) cgcgcgCTGCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAg < 1:3506093/70‑1 (MQ=255) cgcgcgCTGCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAg < 1:2029183/70‑1 (MQ=255) gcgcgcTGCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGa < 1:3767618/70‑1 (MQ=255) gcgcgcTGCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGa < 1:1969361/70‑1 (MQ=255) cgcgcTGCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGAtt > 1:3424370/1‑71 (MQ=255) gcgcTGCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGAttt > 1:530225/1‑71 (MQ=255) gcgcTGCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGAttt < 1:2219437/71‑1 (MQ=255) gcTGCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTgg > 1:3348385/1‑71 (MQ=255) gcTGCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTg > 1:3989178/1‑70 (MQ=255) gCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGa < 1:1990640/63‑1 (MQ=255) gCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGgcg > 1:3504686/1‑70 (MQ=255) gCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGgcg > 1:2212074/1‑70 (MQ=255) aGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAAt < 1:1565845/71‑1 (MQ=255) aaCCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGa < 1:4013855/71‑1 (MQ=255) ccGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGa > 1:3212140/1‑69 (MQ=255) gCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGAttt > 1:1914644/1‑57 (MQ=255) gCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGAttt > 1:1065450/1‑57 (MQ=255) gCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGAttt > 1:3779565/1‑57 (MQ=255) gCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATc < 1:3944165/71‑1 (MQ=255) cttcttCGAGGTTATTTTCCGCATGCTCACAGAGTATTTCCAGCTCTACCAGATTTGGCGCa < 1:2419440/62‑1 (MQ=255) tcttcGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCt > 1:4780178/1‑71 (MQ=255) ttcGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATc > 1:3552382/1‑66 (MQ=255) tcGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTgg > 1:4055866/1‑70 (MQ=255) cGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGata > 1:3335188/1‑62 (MQ=255) cGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTgg > 1:3662643/1‑69 (MQ=255) cGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTgg > 1:1436471/1‑69 (MQ=255) gTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTggcaggca < 1:1392406/71‑1 (MQ=255) ttATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTTGCGCAATGATATCGCTGGCAGGCaa < 1:3978969/71‑1 (MQ=255) ttATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCaa < 1:3982527/52‑1 (MQ=255) ttATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAAt < 1:3412798/53‑1 (MQ=255) ttATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAAt < 1:1385300/53‑1 (MQ=255) ttATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCaa < 1:1002229/71‑1 (MQ=255) ttATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCaa < 1:1490134/71‑1 (MQ=255) aTTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAAc > 1:4334920/1‑71 (MQ=255) aTTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAAc > 1:1278629/1‑71 (MQ=255) tttACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAAcc < 1:2573541/71‑1 (MQ=255) tttACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAAcc < 1:2319943/71‑1 (MQ=255) tttACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAAcc > 1:474974/1‑71 (MQ=255) tACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCg < 1:261673/70‑1 (MQ=255) aCCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCTCAATGATATCGCTGGCAGGCAAACCGt < 1:1781302/70‑1 (MQ=255) aTGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGca > 1:748577/1‑71 (MQ=255) aTGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGca > 1:222529/1‑71 (MQ=255) tGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTgg < 1:910483/52‑1 (MQ=255) tCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAAt < 1:3828639/38‑1 (MQ=255) tCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGG‑AGGCAAACCGTg > 1:4687688/1‑61 (MQ=255) acaGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCTCATGa < 1:3515697/70‑1 (MQ=255) acaGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGa < 1:4863880/70‑1 (MQ=255) caGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGt < 1:2745793/58‑1 (MQ=255) agagTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGc > 1:3255957/1‑42 (MQ=255) agagTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGaaa > 1:3909117/1‑70 (MQ=255) agagTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGaaa > 1:2868878/1‑70 (MQ=255) agagTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAAc > 1:2111558/1‑71 (MQ=255) agagTATTTCCAGCTCAACCAGATTTGGCGCAAAGata > 1:3465503/1‑38 (MQ=255) agagTATTTCCAGCTAAACCAGATTTGGCGCAATGATATCGCTGGCAGGAAAACCGTGCCGCACATGaaa > 1:461354/1‑70 (MQ=255) gagTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAAct > 1:3170211/1‑71 (MQ=255) agTATTTCCAGCTCAATCAGATTTGGCGCAATGat < 1:1018209/35‑1 (MQ=255) agTATTTCCAGCTCAATCAGATTTGGCGCAATGat < 1:1635533/35‑1 (MQ=255) tATTTCCAGCTCAATCAGATTTGGCGCAATGatat > 1:2047708/1‑35 (MQ=255) tATTTCCAGCTCAATCAGATTTGGCGCAATGatat > 1:2998348/1‑35 (MQ=255) tttCCAGCTCAACCAGATTTGGCGCAATGATATCGCTggc > 1:1448550/1‑40 (MQ=255) tttCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGc < 1:4056438/70‑1 (MQ=255) tttCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCg < 1:3655110/71‑1 (MQ=255) tttCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCg < 1:3197445/71‑1 (MQ=255) tCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGa > 1:4219769/1‑70 (MQ=255) aGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACAcc < 1:4646084/71‑1 (MQ=255) gCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACAccc > 1:4526693/1‑71 (MQ=255) gCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACAccc < 1:2755693/71‑1 (MQ=255) tCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAActct < 1:2901950/60‑1 (MQ=255) aCCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGc < 1:2913817/71‑1 (MQ=255) ccAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGc > 1:466050/1‑41 (MQ=255) ccAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGc > 1:4519659/1‑41 (MQ=255) ccAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGc > 1:3542490/1‑41 (MQ=255) | AGTTCGCGCGCTGCCAGAACCGCTTCTTCGACGTTATTTACCGCATGCTCACAGAGTATTTCCAGCTCAACCAGATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGC > W3110S.gb/1717092‑1717231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |