| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,724,748 | T→C | 26.8% | V305A (GTC→GCC) | ydhK → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,724,748 | 0 | T | C | 26.8% | 62.8 / 26.9 | 41 | V305A (GTC→GCC) | ydhK | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (17/13); new base C (5/6); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGTTCGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGT > W3110S.gb/1724686‑1724818 | cAGTTCGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGCCGCCGACt < 1:1628882/71‑1 (MQ=255)cAGTTCGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGCCGCCGACt < 1:4985565/71‑1 (MQ=255) aGTTCGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTa < 1:824971/71‑1 (MQ=255) tCGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTAt > 1:4427037/1‑69 (MQ=255) tCGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGCCGCCGACTATCg > 1:748814/1‑71 (MQ=255) cAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAAcgccgc > 1:4464669/1‑59 (MQ=255) cAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAAcgccgc > 1:256931/1‑59 (MQ=255) cAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAAcgccgc > 1:250801/1‑59 (MQ=255) aaaCAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCAc < 1:3546296/71‑1 (MQ=255) aCAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGt > 1:1323931/1‑71 (MQ=255) gTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCtt > 1:5098088/1‑71 (MQ=255) ttACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCt < 1:1885869/68‑1 (MQ=255) ttACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCt < 1:3736853/68‑1 (MQ=255) tACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGCCGCCGACTATCGGCACGTCGCCTTCTg > 1:2345969/1‑71 (MQ=255) cGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGc < 1:3226147/71‑1 (MQ=255) cGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGc < 1:2155287/71‑1 (MQ=255) gCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGAc > 1:5001419/1‑70 (MQ=255) tattatCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGt > 1:1099309/1‑70 (MQ=255) attatCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTa > 1:397323/1‑71 (MQ=255) attatCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTa > 1:4492415/1‑71 (MQ=255) ttatCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTa < 1:3251137/70‑1 (MQ=255) ttatCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTAt < 1:3201870/71‑1 (MQ=255) ttatCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTAt < 1:3665747/71‑1 (MQ=255) ttatCGCCCCGCTACGCCCGACCAACGCCGCCGACTATCgg < 1:2907746/41‑1 (MQ=255) ttatCGCCCCGCTACGCCCGACCAACGCCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTAt > 1:2356172/1‑71 (MQ=255) atCGCCCCGCTGCGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTAttt > 1:3738196/1‑71 (MQ=255) atCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTAttt > 1:886915/1‑71 (MQ=255) atCGCCCCGCTACGCCCGACCAACGCCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTAttt > 1:3126299/1‑71 (MQ=255) tCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTAtttt < 1:288772/71‑1 (MQ=255) gCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGCCTACGTTAttttt < 1:5013714/70‑1 (MQ=255) gCCCCGCTACGCCCGACCAACGCCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTAttttt < 1:634363/70‑1 (MQ=255) gCCCCGCTACGCCCGACCAACGCCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTAttttt < 1:2405494/70‑1 (MQ=255) ccGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTgcc > 1:3118554/1‑70 (MQ=255) cGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGa > 1:1426729/1‑53 (MQ=255) aCGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCtt > 1:10999/1‑70 (MQ=255) aCGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCtt > 1:2764212/1‑70 (MQ=255) cGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCttt < 1:4955413/70‑1 (MQ=255) cccGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATc < 1:3193417/71‑1 (MQ=255) gACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTgc > 1:52292/1‑58 (MQ=255) aCCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGc > 1:4325007/1‑70 (MQ=255) ccAACGCCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTg > 1:4521006/1‑55 (MQ=255) aaCGCCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAag < 1:2135074/71‑1 (MQ=255) aCGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTgcc > 1:4241372/1‑54 (MQ=255) aCGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATc > 1:2686055/1‑63 (MQ=255) tCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCa < 1:2617967/64‑1 (MQ=255) tCGCCGACTATCGGCACGTCGCCTTCTGGCAGCAACTACGTTATTTTTGCCGCCTTTATCTGCAAagtagt > 1:2190085/1‑71 (MQ=255) | CAGTTCGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGT > W3110S.gb/1724686‑1724818 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |