| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,745,181 | A→G | 40.7% | Y4C (TAT→TGT) | mdtK → | multidrug efflux system transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,745,181 | 0 | A | G | 40.7% | 25.8 / 64.9 | 54 | Y4C (TAT→TGT) | mdtK | multidrug efflux system transporter |
| Reads supporting (aligned to +/- strand): ref base A (15/17); new base G (10/12); total (25/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
CCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCA > W3110S.gb/1745118‑1745244 | ccATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGt > 1:2994761/1‑71 (MQ=255) aTTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGa < 1:1666031/71‑1 (MQ=255) ttCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGt > 1:2367250/1‑68 (MQ=255) ttCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTg > 1:3947059/1‑69 (MQ=255) ttCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTg > 1:2875435/1‑69 (MQ=255) cAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAgc > 1:4837396/1‑71 (MQ=255) cAGGAGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAgc > 1:1543471/1‑71 (MQ=255) cTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTg < 1:3551557/71‑1 (MQ=255) ttttAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGt > 1:2088693/1‑70 (MQ=255) ttttACTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGt > 1:3434825/1‑66 (MQ=255) tttAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGtta > 1:757531/1‑71 (MQ=255) tttAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGtta > 1:546276/1‑71 (MQ=255) ttAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAg > 1:1552380/1‑59 (MQ=255) gTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTattag < 1:4462132/71‑1 (MQ=255) gTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGAGTCTGTTattag < 1:170823/71‑1 (MQ=255) atatAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTattagcattag > 1:2153560/1‑70 (MQ=255) atatAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTattagcat < 1:1037769/67‑1 (MQ=255) atatAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTattagcat < 1:4129527/67‑1 (MQ=255) atatAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTattagcat < 1:4123804/67‑1 (MQ=255) tataACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAg > 1:1048326/1‑43 (MQ=255) tataACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAg > 1:2795895/1‑43 (MQ=255) taACTACAAATAAAAGGTGTTCACGTGCAGAAGTatat < 1:681470/38‑1 (MQ=255) taACTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAAt < 1:5129429/71‑1 (MQ=255) taACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAgcgc > 1:3296431/1‑49 (MQ=255) aCTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCACTcc > 1:3923238/1‑71 (MQ=255) aCTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATcc > 1:3011314/1‑71 (MQ=255) aCTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATcc > 1:1340976/1‑71 (MQ=255) cTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGa < 1:583702/41‑1 (MQ=255) cAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGt < 1:4423548/71‑1 (MQ=255) cAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGt < 1:2813982/71‑1 (MQ=255) cAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGt > 1:4687768/1‑71 (MQ=255) aaaTAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGa < 1:4323958/37‑1 (MQ=255) aaTAAAAGGTGTTCACGTGCAGAAGTATATCATTGAAGCGCGTc < 1:4524450/44‑1 (MQ=255) aaTAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTc < 1:609971/44‑1 (MQ=255) aaTAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTc < 1:4276704/44‑1 (MQ=255) tAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGAtt > 1:511397/1‑71 (MQ=255) tAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCAAGGTGAt > 1:3298682/1‑70 (MQ=255) aaaGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGttatta < 1:3109729/48‑1 (MQ=255) aaaGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGttatta < 1:5019640/48‑1 (MQ=255) aaGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATtctc < 1:2642492/71‑1 (MQ=255) aaGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATtct < 1:608713/70‑1 (MQ=255) aGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTg < 1:1883726/40‑1 (MQ=255) ggTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTattagcattagc < 1:320276/53‑1 (MQ=255) tgtTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGt < 1:3714168/38‑1 (MQ=255) gtTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCgcgc > 1:4780401/1‑70 (MQ=255) ttCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTattagcattag < 1:220133/48‑1 (MQ=255) ttCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTattagcattag < 1:4691270/48‑1 (MQ=255) ttCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTattagcattag < 1:346421/48‑1 (MQ=255) ttCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCaa < 1:2789091/71‑1 (MQ=255) cACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCaaa > 1:380220/1‑70 (MQ=255) cACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAAt > 1:2117486/1‑71 (MQ=255) cACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAAt > 1:674630/1‑71 (MQ=255) cAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCa < 1:3644146/71‑1 (MQ=255) gTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCa < 1:2528918/57‑1 (MQ=255) gTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCa < 1:648488/57‑1 (MQ=255) | CCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCA > W3110S.gb/1745118‑1745244 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |