Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 1,746,430 | C→T | 10.1% | G420G (GGC→GGT) | mdtK → | multidrug efflux system transporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 1,746,430 | 0 | C | T | 10.1% | 179.0 / 8.2 | 59 | G420G (GGC→GGT) | mdtK | multidrug efflux system transporter |
Reads supporting (aligned to +/- strand): ref base C (23/30); new base T (3/3); total (26/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCT > W3110S.gb/1746372‑1746498 | cAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTg < 1:3066389/64‑1 (MQ=255) cAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTg < 1:4548023/64‑1 (MQ=255) aGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCtt < 1:2093280/71‑1 (MQ=255) aGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCtt < 1:2087344/71‑1 (MQ=255) gCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCtt > 1:2569795/1‑70 (MQ=255) gCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTttatt < 1:4786372/71‑1 (MQ=255) cTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGc > 1:3113470/1‑52 (MQ=255) cTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGTTTCTGGATAGGCTttatt < 1:1167424/70‑1 (MQ=255) cTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTttatta < 1:743007/71‑1 (MQ=255) cTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTttatta > 1:1721468/1‑71 (MQ=255) tatTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTttatt < 1:917423/67‑1 (MQ=255) tatTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTg > 1:4785188/1‑71 (MQ=255) tatTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTg > 1:3183336/1‑71 (MQ=255) ttCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGTCCAGCAGGCTTCTGGATAGGCTTtattat > 1:2670661/1‑67 (MQ=255) tCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCtt > 1:1354836/1‑48 (MQ=255) cTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCt > 1:1421908/1‑71 (MQ=255) cTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCt > 1:3908599/1‑71 (MQ=255) cTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCt > 1:969424/1‑71 (MQ=255) gCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGAc < 1:2152325/71‑1 (MQ=255) gCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGAc < 1:2158579/71‑1 (MQ=255) cTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGc < 1:4748684/51‑1 (MQ=255) cTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGc < 1:1618204/51‑1 (MQ=255) cTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGc < 1:2190982/51‑1 (MQ=255) cTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGcc < 1:4991245/64‑1 (MQ=255) cTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGcc < 1:522109/64‑1 (MQ=255) tGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTgg < 1:1173356/44‑1 (MQ=255) gACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGa > 1:1063630/1‑44 (MQ=255) gACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGa > 1:891460/1‑44 (MQ=255) gACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGa > 1:1896742/1‑44 (MQ=255) gACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGa > 1:1243457/1‑44 (MQ=255) aCCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGc > 1:758471/1‑36 (MQ=255) aCCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCgg > 1:2370824/1‑70 (MQ=255) cGATCTGGTCGTTGAACCTATGGGGCCAGCAGGTTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAg > 1:3944468/1‑71 (MQ=255) aTCTGGTCGTTGAACCTATGGGGTCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGc > 1:4360015/1‑70 (MQ=255) gTCGTTGCACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTat > 1:2906072/1‑71 (MQ=255) gTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCAt < 1:2490015/68‑1 (MQ=255) gTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCAt < 1:1546857/68‑1 (MQ=255) gTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCAt < 1:1382970/68‑1 (MQ=255) gTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTat < 1:1682362/71‑1 (MQ=255) gTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTat < 1:4902196/71‑1 (MQ=255) tGAACCTATGGGGCCAGCAGGTTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgatga > 1:3272464/1‑71 (MQ=255) gAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgatga > 1:856763/1‑70 (MQ=255) ccTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGtt > 1:2682509/1‑71 (MQ=255) cTATGGGGCCAGCAGGCTTCTGGATAGGCTttatt < 1:176269/35‑1 (MQ=255) ggggCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgat < 1:3152167/60‑1 (MQ=255) gggCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGc < 1:4073924/67‑1 (MQ=255) gggCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTa < 1:3614268/70‑1 (MQ=255) gggCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTa < 1:2002235/70‑1 (MQ=255) gggCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTAt > 1:4167753/1‑71 (MQ=255) gggCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTAt > 1:4273749/1‑71 (MQ=255) ggCCAGCAGGTTTCTGGATAGGCTTTATTATTGGCATGACGTCGGCAGCCATTATGATGATGTTGCg < 1:4676623/67‑1 (MQ=255) ggCCAGCAGGTTTCTGGATAGGCTTTATTATTGGCATGACGTCGGCAGCCATTATGATGATGTTGCg < 1:4670808/67‑1 (MQ=255) cagcagGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgat < 1:4964105/55‑1 (MQ=255) gcagGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgatgat < 1:2241777/56‑1 (MQ=255) gcagGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgatgat < 1:266474/56‑1 (MQ=255) gcagGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgatgat < 1:3894803/56‑1 (MQ=255) cagGTTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTc > 1:4230341/1‑71 (MQ=255) cagGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCgg > 1:4496086/1‑68 (MQ=255) cagGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTc < 1:886574/71‑1 (MQ=255) agGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgatgatg > 1:4736476/1‑55 (MQ=255) gCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCAtaa > 1:3038287/1‑43 (MQ=255) cTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTa < 1:3872732/44‑1 (MQ=255) cTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCt > 1:2035639/1‑69 (MQ=255) cTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCt > 1:1019372/1‑69 (MQ=255) | CAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCT > W3110S.gb/1746372‑1746498 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |