Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,746,430 C→T 10.1% G420G (GGC→GGT mdtK → multidrug efflux system transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,746,4300CT10.1% 179.0 / 8.2 59G420G (GGC→GGTmdtKmultidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base C (23/30);  new base T (3/3);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCT  >  W3110S.gb/1746372‑1746498
                                                          |                                                                    
cAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTg                                                                 <  1:3066389/64‑1 (MQ=255)
cAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTg                                                                 <  1:4548023/64‑1 (MQ=255)
  aGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCtt                                                        <  1:2093280/71‑1 (MQ=255)
  aGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCtt                                                        <  1:2087344/71‑1 (MQ=255)
   gCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCtt                                                        >  1:2569795/1‑70 (MQ=255)
      gCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTttatt                                                    <  1:4786372/71‑1 (MQ=255)
       cTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGc                                                                      >  1:3113470/1‑52 (MQ=255)
       cTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGTTTCTGGATAGGCTttatt                                                    <  1:1167424/70‑1 (MQ=255)
       cTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTttatta                                                   <  1:743007/71‑1 (MQ=255)
       cTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTttatta                                                   >  1:1721468/1‑71 (MQ=255)
          tatTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTttatt                                                    <  1:917423/67‑1 (MQ=255)
          tatTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTg                                                >  1:4785188/1‑71 (MQ=255)
          tatTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTg                                                >  1:3183336/1‑71 (MQ=255)
            ttCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGTCCAGCAGGCTTCTGGATAGGCTTtattat                                                  >  1:2670661/1‑67 (MQ=255)
             tCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCtt                                                                    >  1:1354836/1‑48 (MQ=255)
              cTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCt                                            >  1:1421908/1‑71 (MQ=255)
              cTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCt                                            >  1:3908599/1‑71 (MQ=255)
              cTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCt                                            >  1:969424/1‑71 (MQ=255)
                 gCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGAc                                         <  1:2152325/71‑1 (MQ=255)
                 gCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGAc                                         <  1:2158579/71‑1 (MQ=255)
                    cTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGc                                                          <  1:4748684/51‑1 (MQ=255)
                    cTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGc                                                          <  1:1618204/51‑1 (MQ=255)
                    cTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGc                                                          <  1:2190982/51‑1 (MQ=255)
                    cTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGcc                                             <  1:4991245/64‑1 (MQ=255)
                    cTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGcc                                             <  1:522109/64‑1 (MQ=255)
                     tGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTgg                                                                <  1:1173356/44‑1 (MQ=255)
                      gACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGa                                                               >  1:1063630/1‑44 (MQ=255)
                      gACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGa                                                               >  1:891460/1‑44 (MQ=255)
                      gACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGa                                                               >  1:1896742/1‑44 (MQ=255)
                      gACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGa                                                               >  1:1243457/1‑44 (MQ=255)
                       aCCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGc                                                                      >  1:758471/1‑36 (MQ=255)
                       aCCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCgg                                    >  1:2370824/1‑70 (MQ=255)
                         cGATCTGGTCGTTGAACCTATGGGGCCAGCAGGTTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAg                                 >  1:3944468/1‑71 (MQ=255)
                           aTCTGGTCGTTGAACCTATGGGGTCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGc                                >  1:4360015/1‑70 (MQ=255)
                                gTCGTTGCACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTat                          >  1:2906072/1‑71 (MQ=255)
                                gTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCAt                             <  1:2490015/68‑1 (MQ=255)
                                gTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCAt                             <  1:1546857/68‑1 (MQ=255)
                                gTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCAt                             <  1:1382970/68‑1 (MQ=255)
                                gTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTat                          <  1:1682362/71‑1 (MQ=255)
                                gTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTat                          <  1:4902196/71‑1 (MQ=255)
                                     tGAACCTATGGGGCCAGCAGGTTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgatga                     >  1:3272464/1‑71 (MQ=255)
                                      gAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgatga                     >  1:856763/1‑70 (MQ=255)
                                         ccTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGtt                 >  1:2682509/1‑71 (MQ=255)
                                          cTATGGGGCCAGCAGGCTTCTGGATAGGCTttatt                                                    <  1:176269/35‑1 (MQ=255)
                                              ggggCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgat                       <  1:3152167/60‑1 (MQ=255)
                                               gggCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGc               <  1:4073924/67‑1 (MQ=255)
                                               gggCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTa            <  1:3614268/70‑1 (MQ=255)
                                               gggCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTa            <  1:2002235/70‑1 (MQ=255)
                                               gggCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTAt           >  1:4167753/1‑71 (MQ=255)
                                               gggCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTAt           >  1:4273749/1‑71 (MQ=255)
                                                ggCCAGCAGGTTTCTGGATAGGCTTTATTATTGGCATGACGTCGGCAGCCATTATGATGATGTTGCg              <  1:4676623/67‑1 (MQ=255)
                                                ggCCAGCAGGTTTCTGGATAGGCTTTATTATTGGCATGACGTCGGCAGCCATTATGATGATGTTGCg              <  1:4670808/67‑1 (MQ=255)
                                                   cagcagGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgat                       <  1:4964105/55‑1 (MQ=255)
                                                     gcagGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgatgat                    <  1:2241777/56‑1 (MQ=255)
                                                     gcagGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgatgat                    <  1:266474/56‑1 (MQ=255)
                                                     gcagGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgatgat                    <  1:3894803/56‑1 (MQ=255)
                                                      cagGTTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTc    >  1:4230341/1‑71 (MQ=255)
                                                      cagGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCgg       >  1:4496086/1‑68 (MQ=255)
                                                      cagGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTc    <  1:886574/71‑1 (MQ=255)
                                                       agGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgatgatg                   >  1:4736476/1‑55 (MQ=255)
                                                         gCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCAtaa                           >  1:3038287/1‑43 (MQ=255)
                                                          cTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTa                           <  1:3872732/44‑1 (MQ=255)
                                                          cTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCt  >  1:2035639/1‑69 (MQ=255)
                                                          cTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCt  >  1:1019372/1‑69 (MQ=255)
                                                          |                                                                    
CAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCT  >  W3110S.gb/1746372‑1746498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: