Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,754,025	G→A	31.3%	P507L (CCA→CTA)	ydhV ←	predicted oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,754,025	0	G	A	31.3%	94.2 / 56.5	67	P507L (CCA→CTA)	ydhV	predicted oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (22/24);  new base A (17/4);  total (39/28)
Fisher's exact test for biased strand distribution p-value = 1.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

TTTGGTTTCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAA  >  W3110S.gb/1753958‑1754091                                                                     |                                                                   tttGGTTTCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGc                                                                  >  1:2630253/1‑70 (MQ=255)   tGGTTTCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCaa                                                                <  1:950562/70‑1 (MQ=255)        tCATCGTAAGCATCTTCAGAACCAAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCa                                                                 <  1:4291629/65‑1 (MQ=255)        tCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCgg                                                           <  1:1160458/70‑1 (MQ=255)        tCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCgg                                                           >  1:4745025/1‑70 (MQ=255)        tCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCgg                                                           >  1:3915792/1‑70 (MQ=255)        tCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCgg                                                           >  1:3662717/1‑70 (MQ=255)        tCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCgg                                                           >  1:1524277/1‑70 (MQ=255)         cATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCgg                                                           <  1:4392616/69‑1 (MQ=255)            cGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGc                                                                  >  1:393709/1‑59 (MQ=255)            cGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAAcc                                                       >  1:2091474/1‑70 (MQ=255)                aGCATCTTCAGAACCAAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGAACCaa                                                     >  1:3641042/1‑69 (MQ=255)                aGCATCTTCAGAACCAAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGAACCaa                                                     >  1:4356575/1‑69 (MQ=255)                aGCATCTTCAGAACCAAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGAACCAAt                                                    >  1:899574/1‑70 (MQ=255)                aGCATCTTCAGAACCAAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGAACCAAt                                                    >  1:4082021/1‑70 (MQ=255)                aGCATCTTCAGAACCAAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGAACCAATa                                                   <  1:1810457/71‑1 (MQ=255)                aGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATaa                                                  <  1:4273136/71‑1 (MQ=255)                aGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATaa                                                  <  1:4309534/71‑1 (MQ=255)                aGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATaa                                                  <  1:4798503/71‑1 (MQ=255)                  cATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCgg                                                           <  1:1428444/60‑1 (MQ=255)                  cATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATaa                                                  <  1:2188758/69‑1 (MQ=255)                   atcatcAGAACCAAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGc                                                              >  1:3481547/1‑57 (MQ=255)                       tCAGAACCAAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGAACCAATAAAGTTg                                             >  1:4240355/1‑70 (MQ=255)                       tCAGAACCAAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGAACCAATAAAGTTGa                                            >  1:3836369/1‑71 (MQ=255)                       tCAGAACCAAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGAACCAATAAAGTTGa                                            >  1:1540260/1‑71 (MQ=255)                       tCAGAACCAAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGAACCAATAAAGTTGa                                            >  1:2926544/1‑71 (MQ=255)                        cAGAACCAAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGAACCAATAAAGTTGa                                            <  1:1471443/70‑1 (MQ=255)                        cAGAACCAAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGAACCAATAAAGTTGa                                            >  1:3207101/1‑70 (MQ=255)                        cAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGtt                                              <  1:28204/67‑1 (MQ=255)                        cAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGa                                            <  1:3639460/69‑1 (MQ=255)                            aCC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATgg                                                                   >  1:3571840/1‑42 (MQ=255)                            aCC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATggg                                      <  1:492815/71‑1 (MQ=255)                                 aaaaGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATg                                  >  1:2882143/1‑71 (MQ=255)                                  aaaGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGaa                                                         >  1:4826531/1‑47 (MQ=255)                                  aaaGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGaa                                                         >  1:4657200/1‑47 (MQ=255)                                  aaaGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGaa                                                         >  1:4402382/1‑47 (MQ=255)                                   aaGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCg                                <  1:2010249/71‑1 (MQ=255)                                           tCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGc                          <  1:302245/69‑1 (MQ=255)                                                aCTTCACGTTGCAGTTTCAATAGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACg                   >  1:4175939/1‑71 (MQ=255)                                                  ttCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTg                                             <  1:1974572/43‑1 (MQ=255)                                                  ttCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTg                                             <  1:5067740/43‑1 (MQ=255)                                                  ttCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTg                                             <  1:1305993/43‑1 (MQ=255)                                                  ttCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGt                               <  1:4601182/57‑1 (MQ=255)                                                   tCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGtt                >  1:3140483/1‑71 (MQ=255)                                                   tCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGtt                >  1:490897/1‑71 (MQ=255)                                                   tCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGtt                >  1:2109874/1‑71 (MQ=255)                                                   tCACGTTGCAGTTTCAATAGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGt                 >  1:2147133/1‑70 (MQ=255)                                                   tCACGTTGCAGTTTCAATAGCAAGCCGGAACAAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGtt                >  1:3251479/1‑71 (MQ=255)                                                    cACGTTGCAGTTTCAATAGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGtt                >  1:2093664/1‑70 (MQ=255)                                                     aCGTTGCAGTTTCAATAGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGa              <  1:2117772/71‑1 (MQ=255)                                                          gCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACAt          <  1:1166488/70‑1 (MQ=255)                                                              tttCAATGGCAAGCCGGAACCAATAAAGTTGATATggg                                      <  1:603826/38‑1 (MQ=255)                                                              tttCAATGGCAAGCCGGAACCAATAAAGTTGATATgg                                       <  1:4664316/37‑1 (MQ=255)                                                              tttCAATGGCAAGCCGGAACCAATAAAGTTGATATgg                                       <  1:1417654/37‑1 (MQ=255)                                                              tttCAATGGCAAGCCGGAACCAATAAAGTTGATATgg                                       <  1:1272451/37‑1 (MQ=255)                                                              tttCAATGGCAAGCCGGAACCAATAAAGTTGATATgg                                       <  1:1051309/37‑1 (MQ=255)                                                              tttCAATGGCAAGCCGGAACCAATAAAGTTGATATgg                                       <  1:2058887/37‑1 (MQ=255)                                                              tttCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCa                     >  1:4089340/1‑55 (MQ=255)                                                              tttCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCa                     >  1:544670/1‑55 (MQ=255)                                                              tttCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCa                     >  1:772165/1‑55 (MQ=255)                                                                tCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGt                                     >  1:752358/1‑37 (MQ=255)                                                                tCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCAt                            >  1:1691056/1‑46 (MQ=255)                                                                tCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCAt                            >  1:895385/1‑46 (MQ=255)                                                                tCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGtt    >  1:1559106/1‑70 (MQ=255)                                                                tCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGtt    >  1:406535/1‑70 (MQ=255)                                                                  agtGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGt                 >  1:1789547/3‑55 (MQ=255)                                                                  aaTGGCAAGCCGGAACCAATAAAGTTGATATGGGt                                     >  1:826811/1‑35 (MQ=255)                                                                  aaTGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTaa  >  1:4252549/1‑70 (MQ=255)                                                                     |                                                                   TTTGGTTTCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAA  >  W3110S.gb/1753958‑1754091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑