| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,874,182 | A→G | 20.0% | Y143C (TAC→TGC) | yeaJ → | predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,874,182 | 0 | A | G | 20.0% | 117.8 / 24.8 | 55 | Y143C (TAC→TGC) | yeaJ | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (25/19); new base G (9/2); total (34/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTAT > W3110S.gb/1874114‑1874252 | tgtgGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCg > 1:805491/1‑71 (MQ=255) gtgGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACgg > 1:424133/1‑71 (MQ=255) aaGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGtt < 1:3099386/69‑1 (MQ=255) aGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAg < 1:4235728/71‑1 (MQ=255) aGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAg < 1:1585004/71‑1 (MQ=255) gcaCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTa > 1:2877172/1‑56 (MQ=255) gcaCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTgg > 1:3921494/1‑71 (MQ=255) gcaCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTgg < 1:3145163/71‑1 (MQ=255) cTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGa > 1:548318/1‑70 (MQ=255) cTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGa > 1:3045783/1‑70 (MQ=255) cTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGa > 1:3682977/1‑70 (MQ=255) aTTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATgg > 1:2759292/1‑71 (MQ=255) aCCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGcc > 1:4197906/1‑70 (MQ=255) aCCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGcc > 1:132282/1‑70 (MQ=255) cccGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCg > 1:1670796/1‑70 (MQ=255) cccGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCg > 1:1611585/1‑70 (MQ=255) cccGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCg > 1:3887574/1‑70 (MQ=255) ccGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGaa < 1:3340990/71‑1 (MQ=255) ccGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGaa < 1:1231452/71‑1 (MQ=255) ccGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGaa < 1:1992890/71‑1 (MQ=255) cGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCg > 1:1787164/1‑68 (MQ=255) cGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGaa < 1:3965565/70‑1 (MQ=255) gCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGa > 1:3616785/1‑61 (MQ=255) gCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGaa > 1:4394330/1‑69 (MQ=255) cTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAagag < 1:4475463/71‑1 (MQ=255) ttGATCAGTCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAgg > 1:156843/1‑49 (MQ=255) ttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTa > 1:2139802/1‑40 (MQ=255) aGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACtat > 1:3316534/1‑70 (MQ=255) ggAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTAtc > 1:1583428/1‑71 (MQ=255) gAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTgg > 1:3783933/1‑41 (MQ=255) gAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTgg > 1:3692006/1‑41 (MQ=255) gAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGaa > 1:3028148/1‑51 (MQ=255) aCCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGAtct > 1:2804919/1‑70 (MQ=255) aCCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGAtctc < 1:450478/71‑1 (MQ=255) ccGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCa < 1:2656255/71‑1 (MQ=255) cGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAAtt > 1:78905/1‑50 (MQ=255) cGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCaa > 1:3378141/1‑71 (MQ=255) gCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAAt < 1:3183324/71‑1 (MQ=255) accaGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCaa > 1:1071814/1‑67 (MQ=255) accaGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCaa > 1:1325215/1‑67 (MQ=255) accaGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGAc < 1:374875/71‑1 (MQ=255) accaGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGAc < 1:3138371/71‑1 (MQ=255) caGGATGATTACGGTTCAGGTCTTGGGATGGCCGa < 1:701696/35‑1 (MQ=255) caGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGAt > 1:2660167/1‑60 (MQ=255) caGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGAt > 1:4920366/1‑60 (MQ=255) caGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATATCACTATTATCTCGAt > 1:2505926/1‑60 (MQ=255) aGGATGATTACGGTTCAGGTCTTTGGATGGCCGAAGAGAAATTTCACta > 1:1174594/1‑49 (MQ=255) aGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGaaa < 1:4271932/41‑1 (MQ=255) aGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGaaa < 1:5081759/41‑1 (MQ=255) aGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACta > 1:4692868/1‑49 (MQ=255) ggATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGc > 1:3958004/1‑70 (MQ=255) gatgatTACGGTTCAGGTCTTGGGATGGCGGAAGAGAAATTTCAc < 1:761343/45‑1 (MQ=255) gatgatTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCAc < 1:4942196/45‑1 (MQ=255) tgatTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGAt > 1:1166765/1‑55 (MQ=255) gatTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACtattat > 1:4423350/1‑48 (MQ=255) atTGCGGTTCAGGTCTTGGGATGGCCTAAGAGAAATTTCACTATTAtc < 1:5140272/48‑1 (MQ=255) atTGCGGGTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTAtc < 1:1590775/48‑1 (MQ=255) aCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTAt > 1:2464542/1‑71 (MQ=255) | TGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTAT > W3110S.gb/1874114‑1874252 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |