| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,903,275 | T→C | 7.0% | I9V (ATT→GTT) | yoaE ← | fused predicted membrane proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,903,275 | 0 | T | C | 7.0% | 161.9 / 2.9 | 57 | I9V (ATT→GTT) | yoaE | fused predicted membrane proteins |
| Reads supporting (aligned to +/- strand): ref base T (30/23); new base C (2/2); total (32/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGAAGACCAGGTTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAAT > W3110S.gb/1903207‑1903344 | gATGAAGACCAGGTTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTg < 1:1365880/71‑1 (MQ=255)gATGAAGACCAGGTTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTg < 1:2051521/71‑1 (MQ=255) aTGAAGACCAGGTTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAAtt < 1:76001/69‑1 (MQ=255) ggTTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCAtt < 1:45386/71‑1 (MQ=255) gTTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAg < 1:1727440/61‑1 (MQ=255) gTTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCAt > 1:3815944/1‑69 (MQ=255) tATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAgg > 1:1050097/1‑60 (MQ=255) tATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAgg > 1:4910818/1‑60 (MQ=255) tATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAgg > 1:2430018/1‑60 (MQ=255) aTCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAg > 1:5047042/1‑58 (MQ=255) tCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGaa > 1:4756946/1‑71 (MQ=255) cGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAAtt > 1:1520364/1‑53 (MQ=255) gATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTATCCCCGCCCAAATTGAGGGGTCCATTAATAAtt < 1:4638522/71‑1 (MQ=255) gATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTTAGaa > 1:1231024/1‑69 (MQ=255) gATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAAtt > 1:2261841/1‑71 (MQ=255) tACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTcc < 1:2607597/71‑1 (MQ=255) tACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTcc < 1:1303486/71‑1 (MQ=255) aCCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAAtt < 1:3108601/49‑1 (MQ=255) aCCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAAtt < 1:3751334/49‑1 (MQ=255) cccAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTg < 1:207523/49‑1 (MQ=255) cccAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTg < 1:2768934/49‑1 (MQ=255) cccAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTg < 1:871738/49‑1 (MQ=255) cccAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCa < 1:827887/70‑1 (MQ=255) aGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAACTGAGGGGTCCATTAAGAATTCCATGAc < 1:1653568/71‑1 (MQ=255) aGCACAATTTCGAGAACAACAAGCGTGAGTAGCCACGCCCAAACTGAGGGGTCCATTAAGAATTCCATGAc < 1:1678521/71‑1 (MQ=255) gCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGAc > 1:3613464/1‑70 (MQ=255) gCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGAc > 1:3930207/1‑70 (MQ=255) gCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACa < 1:2736998/71‑1 (MQ=255) gCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACa < 1:3343436/71‑1 (MQ=255) cacaATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGAc > 1:3619030/1‑69 (MQ=255) caATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGa > 1:4728303/1‑43 (MQ=255) aTTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGAc > 1:3652324/1‑65 (MQ=255) aTTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGAc > 1:565686/1‑65 (MQ=255) ttCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAg > 1:1800711/1‑40 (MQ=255) gagaACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCt > 1:1473805/1‑71 (MQ=255) gagaACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCt > 1:896472/1‑71 (MQ=255) gagaACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCt > 1:1626326/1‑71 (MQ=255) acaacaAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTc < 1:4971827/38‑1 (MQ=255) caacaaGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGaa > 1:4847116/1‑46 (MQ=255) caacaaGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGaa > 1:4257558/1‑46 (MQ=255) caacaaGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAgg < 1:1067350/71‑1 (MQ=255) caacaaGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAgg < 1:3300775/71‑1 (MQ=255) caacaaGCGTGAGTAGCCCCGCCCAAACTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAg > 1:615175/1‑70 (MQ=255) aacaaGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTc < 1:1990217/48‑1 (MQ=255) aacaaGCGTGAGTAGCCCCGCCCAAACTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAgg > 1:4694707/1‑70 (MQ=255) caaGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAAt < 1:2514203/71‑1 (MQ=255) gCGTGAGTAGCCCCGCCCAAATTGAGTGGTCCATTAAGAATTCCATGACa < 1:2477429/50‑1 (MQ=255) gCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGc > 1:4273081/1‑71 (MQ=255) cGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGc > 1:609582/1‑70 (MQ=255) cGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGc > 1:2401615/1‑70 (MQ=255) gTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGa > 1:899467/1‑37 (MQ=255) aGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTc < 1:3508340/71‑1 (MQ=255) tAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGaa > 1:396905/1‑60 (MQ=255) ccGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGc > 1:1498108/1‑69 (MQ=255) ccAAATTGAGGGGTCCATTAAGAATTCCATGACaac > 1:289228/1‑35 (MQ=255) ccAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATa < 1:404771/53‑1 (MQ=255) ccAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGaaa > 1:283264/1‑71 (MQ=255) cAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAgg < 1:1880946/48‑1 (MQ=255) aaaTTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAaata < 1:3882403/71‑1 (MQ=255) aTTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAaataat > 1:2779855/1‑71 (MQ=255) aTTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAaataat > 1:170578/1‑71 (MQ=255) ttGAGGGGTCGATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCt < 1:890327/51‑1 (MQ=255) ttGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCt < 1:161042/51‑1 (MQ=255) | GATGAAGACCAGGTTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAAT > W3110S.gb/1903207‑1903344 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |