| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,953,447 | G→A | 46.0% | A113A (GCG→GCA) | yecE → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,953,447 | 0 | G | A | 46.0% | 14.8 / 64.2 | 50 | A113A (GCG→GCA) | yecE | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (17/10); new base A (9/14); total (26/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCA > W3110S.gb/1953384‑1953510 | tCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTgg > 1:4800654/1‑70 (MQ=255) ccGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCa > 1:4055647/1‑71 (MQ=255) ccGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCACTTTGGCa > 1:2744474/1‑71 (MQ=255) cgcgCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCACTTTGGCa < 1:4085277/70‑1 (MQ=255) cgcgCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCACTTTGGCa < 1:3080273/70‑1 (MQ=255) gcATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCAtttt > 1:2774919/1‑71 (MQ=255) ttGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGTAGCTGCCTGCGCTTTGGCATTTtctc < 1:2894378/71‑1 (MQ=255) ttGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTtctc < 1:4834947/71‑1 (MQ=255) gggCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGa > 1:4090135/1‑71 (MQ=255) gCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCACTTTGGCATTTTCTCGAtt < 1:4576167/71‑1 (MQ=255) cTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCtt > 1:1379817/1‑49 (MQ=255) cTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCtt > 1:529043/1‑49 (MQ=255) cTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTc > 1:986077/1‑71 (MQ=255) cTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCACTTTGGCATTTTCTCGATTCTCtt > 1:416403/1‑70 (MQ=255) cTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCACTTTGGCATTTTCTCGATTCTCTTc > 1:2359152/1‑71 (MQ=255) gCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTg > 1:4116967/1‑71 (MQ=255) tGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCACTTTGGCATTTTCTCGATTCTCTTCCTGGc > 1:2393996/1‑71 (MQ=255) tGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCACTTTGGCATTTTCTCGATTCTCTTCCTGGc > 1:4960125/1‑71 (MQ=255) tGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCACTTTGGCATTTTCTCGATTCTCTTCCTGGc > 1:3395727/1‑71 (MQ=255) ctgcctgcCACATTCGGCCCACGGGAGCTGCCTGCACTTTGGCATTTTCTCGATTCTCTTCCTGGCGAAtt > 1:1821990/1‑71 (MQ=255) gcctgccACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAAtt > 1:2481607/1‑69 (MQ=255) cctgccACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTaa > 1:2234728/1‑71 (MQ=255) ccACATTCGGCCCACGGGAGCTGCCTGCACTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAAtt < 1:1887790/69‑1 (MQ=255) ccACATTCGGCCCACGGGAGCTGCCTGCACTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAAtt < 1:3034764/69‑1 (MQ=255) ccACATTCGGCCCACGGGAGCTGCCTGCACTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAAtt < 1:1585610/69‑1 (MQ=255) caTTCGGCCCACGGGAGCTGCCTGCACTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGc > 1:1193895/1‑71 (MQ=255) aTTCGGCCCACGGGAGCTGCCTGCACTTTGGCATTTtctc < 1:288242/40‑1 (MQ=255) ttCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTtctc < 1:2527472/39‑1 (MQ=255) ttCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGa < 1:2954302/41‑1 (MQ=255) ttCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGa < 1:4404319/41‑1 (MQ=255) tCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGa < 1:1496636/40‑1 (MQ=255) tCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTg > 1:1127975/1‑71 (MQ=255) cGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTtc > 1:313006/1‑35 (MQ=255) ggCCCACGGGAGCTGCCTGCACTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTgg < 1:933019/70‑1 (MQ=255) gCCCACGGGAGCTGCCTGCGCTTTGGCATTTtctc < 1:3116591/35‑1 (MQ=255) ccACGGGAGCTGCCTGCACTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGt < 1:513714/71‑1 (MQ=255) ccACGGGAGCTGCCTGCACTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGt < 1:853970/71‑1 (MQ=255) cACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTaa < 1:84398/55‑1 (MQ=255) aCGGGAGCTGCCTGCACTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAAt > 1:397022/1‑55 (MQ=255) cGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTg > 1:4066087/1‑43 (MQ=255) cGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCg > 1:3372322/1‑71 (MQ=255) gggAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGc > 1:1283618/1‑71 (MQ=255) ggAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGcc < 1:4943957/71‑1 (MQ=255) ctgcctgcACTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAAtt < 1:75983/49‑1 (MQ=255) ctgcctgcACTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATc < 1:4035748/70‑1 (MQ=255) tgcctgcGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAAt < 1:4980035/47‑1 (MQ=255) tgcctgcGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCca > 1:2711621/1‑71 (MQ=255) cctgcGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTa > 1:648741/1‑47 (MQ=255) ctgcACTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAg < 1:4177062/57‑1 (MQ=255) ctgcACTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAg < 1:3581179/57‑1 (MQ=255) cgcTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATcc > 1:4409480/1‑64 (MQ=255) | TCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCA > W3110S.gb/1953384‑1953510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |