| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,987,171 | G→A | 38.9% | G12G (GGC→GGT) | araG ← | fused L‑arabinose transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,987,171 | 0 | G | A | 38.9% | 42.0 / 58.0 | 54 | G12G (GGC→GGT) | araG | fused L‑arabinose transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (22/11); new base A (7/14); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01 | |||||||||||
AACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACAC > W3110S.gb/1987102‑1987237 | aaCCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGc > 1:5045028/1‑71 (MQ=255)aaCCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGc < 1:5057999/71‑1 (MQ=255) ggCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATACCGCGAAATg < 1:3104073/71‑1 (MQ=255) gCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAAt > 1:1876065/1‑69 (MQ=255) gTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATACCGCGAAATGAGAGATACg < 1:4185835/71‑1 (MQ=255) tCAAAACTAATATCCGTCAGCGCCTTAACGCCGGTAAACGTTTTACCGATGCCGCGAAATGAGAGATa < 1:4389947/68‑1 (MQ=255) tCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGaa < 1:4189541/58‑1 (MQ=255) aaaaCTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATACCGCGAAATGAGAGATACgg > 1:3840712/1‑69 (MQ=255) atatCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATACCGCGAAATGAGAGATACGGGGTAGACTg < 1:4165907/71‑1 (MQ=255) atCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATACCGCGAAATGAGAGATACGGGGTAGACTGtt > 1:2124216/1‑71 (MQ=255) cGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATgaga > 1:3333434/1‑50 (MQ=255) cGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTg > 1:1975091/1‑69 (MQ=255) cGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCa > 1:1764931/1‑71 (MQ=255) cGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCa > 1:3136645/1‑71 (MQ=255) gTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATACCGCGAAATGAGAGATACGGGGTAGACTGTTGCAt < 1:1676583/71‑1 (MQ=255) gTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATACCGCGAAATGAGAGATACGGGGTAGACTGTTGCAt < 1:3770383/71‑1 (MQ=255) gTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATACCGCGAAATGAGAGATACGGGGTAGACTGTTGCAt < 1:1462949/71‑1 (MQ=255) tCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATgagaga < 1:1359202/50‑1 (MQ=255) tCAGCGCCTTAACGCCGGGAAACGTTTTACCGATACCGCGAAAt < 1:1182185/44‑1 (MQ=255) cAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATgagaga < 1:522683/49‑1 (MQ=255) cAGCGCCTTAACGCCGGGAAACGTTTTACCGATACCGCGAAAt < 1:1924112/43‑1 (MQ=255) aGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAAc > 1:1889302/1‑71 (MQ=255) aGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGAGAAATGAGAGATACGGGGTAGACTGTTGCATAAc > 1:4722623/1‑71 (MQ=255) aGCGCCTTAACGCCGGGAAACGTTTTACCGATACCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAAc < 1:2621097/71‑1 (MQ=255) cTTAACGCCGGGAAACGTTTTACCGATACCGCGAAATGAGAGATACGGGGTAGa > 1:4682559/1‑54 (MQ=255) cTTAACGCCGGGAAACGTTTTACCGATACCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGtctc > 1:66097/1‑71 (MQ=255) ttAACGCCGGGAAACGTTTTACCGATGCCGCGAAATgaga > 1:1227761/1‑40 (MQ=255) ttAACGCCGGGAAACGTTTTACCGATACCGCGAAATgagaga < 1:4989634/42‑1 (MQ=255) tAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGtt < 1:4105335/57‑1 (MQ=255) tAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCAt > 1:2533345/1‑61 (MQ=255) tAACGCCGGGAAACGTTTTACCGATACCGCGAAATgagaga < 1:70651/41‑1 (MQ=255) tAACGCCGGGAAACGTTTTACCGATACCGCGAAATgagaga < 1:1247949/41‑1 (MQ=255) aaCGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGa > 1:982767/1‑51 (MQ=255) aaCGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGt > 1:3592022/1‑71 (MQ=255) aaCGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGt > 1:3150035/1‑71 (MQ=255) aaCGCCGGGAAACGTTTTACCGATACCGCGAAATGAGAGATa > 1:806751/1‑42 (MQ=255) aCGCCGGGAAACGTTTTACCGATACCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGt < 1:691716/70‑1 (MQ=255) aCGCCGGGAAACGTTTTACCGATACCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTg < 1:2316720/71‑1 (MQ=255) ccGGGAAACGTTTTACCGATGCCGCGAAATgagaga > 1:4626659/1‑36 (MQ=255) ccGGGAAACGTTTTACCGATGCCGCGAAATgagaga > 1:3133045/1‑36 (MQ=255) aaaCGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTg < 1:4620676/71‑1 (MQ=255) aaaCGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTACGTGaa > 1:2889192/1‑65 (MQ=255) cGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCAc > 1:4007380/1‑66 (MQ=255) cGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCAc > 1:163495/1‑66 (MQ=255) cGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCAc > 1:183344/1‑66 (MQ=255) cGTTTTACCGATACCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTc > 1:1618657/1‑71 (MQ=255) tttACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGtt > 1:5043832/1‑71 (MQ=255) tttACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGtt > 1:4323567/1‑71 (MQ=255) ttACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCg < 1:466228/68‑1 (MQ=255) ttACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCg < 1:353706/68‑1 (MQ=255) ttACCGATGCCGAGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGtt < 1:4070558/70‑1 (MQ=255) ttACCGATACCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGtt > 1:4310897/1‑70 (MQ=255) cGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCacac > 1:2033262/1‑71 (MQ=255) gATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGtt < 1:4537507/65‑1 (MQ=255) gCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTg < 1:2821195/48‑1 (MQ=255) | AACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACAC > W3110S.gb/1987102‑1987237 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |