| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,002,152 | T→C | 17.1% | Y124C (TAC→TGC) | fliY ← | cystine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,002,152 | 0 | T | C | 17.1% | 91.9 / 14.1 | 41 | Y124C (TAC→TGC) | fliY | cystine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (12/22); new base C (3/4); total (15/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
GGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAA > W3110S.gb/2002091‑2002221 | ggCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGtt > 1:2690791/1‑70 (MQ=255) ttAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATc > 1:2510370/1‑71 (MQ=255) ttAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGCACGGGGTTGAGAAATc > 1:1942367/1‑71 (MQ=255) tGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTAtttt > 1:360594/1‑71 (MQ=255) gCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTa > 1:12417/1‑48 (MQ=255) gCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTa > 1:2533469/1‑48 (MQ=255) ttCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGt < 1:4659794/62‑1 (MQ=255) ttCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCt > 1:620884/1‑71 (MQ=255) ttCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCt > 1:614780/1‑71 (MQ=255) tCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTTTACGGGGTTgag < 1:2474374/54‑1 (MQ=255) cGTTACCTTTTTTCACCAGCGCCTGAATACCAGGAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTg > 1:4610798/1‑71 (MQ=255) cGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCt < 1:3210749/69‑1 (MQ=255) gTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTg < 1:1067354/70‑1 (MQ=255) aCCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGCACgggg > 1:59208/1‑44 (MQ=255) ccTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCt < 1:2401977/70‑1 (MQ=255) ccTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCt < 1:2441586/70‑1 (MQ=255) ccTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCt < 1:3033182/70‑1 (MQ=255) cTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACgg < 1:1897341/40‑1 (MQ=255) ttttttCACCAGCGCCTGAATACCAGAAATGGTGCAc < 1:4206555/37‑1 (MQ=255) ttttttCACCAGCGCCTGAATACCAGAAATGGTGCAc < 1:1494050/37‑1 (MQ=255) tttttCACCAGCGCCTGAATACCAGAAATGGTGCACGGGGTTGAGaa < 1:4196608/47‑1 (MQ=255) ttttCACCAGCGCCTGAATACCAGAAATGGTGCACgg < 1:1350578/37‑1 (MQ=255) tCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGa < 1:4151604/70‑1 (MQ=255) tCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGa < 1:189386/70‑1 (MQ=255) tCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGa < 1:612258/70‑1 (MQ=255) gcgcCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTg < 1:450242/71‑1 (MQ=255) gcgcCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTg < 1:4364269/71‑1 (MQ=255) tGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATg < 1:3354632/63‑1 (MQ=255) aaTACCAGAAATGGTGTACGGGGTTGAGAAATCGTAtttttt > 1:1875984/1‑42 (MQ=255) tACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTa < 1:4873449/71‑1 (MQ=255) aCCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTaa > 1:4487938/1‑71 (MQ=255) aCCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTaa > 1:3686484/1‑71 (MQ=255) ccAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTaa < 1:805451/70‑1 (MQ=255) ccAGAAATGGTGCACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAAt > 1:2366987/1‑71 (MQ=255) cAGAAATGGTGTACGGGGTTGAGAAATCGTAtttttt > 1:639839/1‑37 (MQ=255) gAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGa < 1:1902746/50‑1 (MQ=255) gAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATcac < 1:2671161/71‑1 (MQ=255) gAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATcac < 1:2788466/71‑1 (MQ=255) aaaTGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGa < 1:1533078/49‑1 (MQ=255) aaaTGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGa < 1:2596850/49‑1 (MQ=255) tGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATcac < 1:2134688/67‑1 (MQ=255) gtgtACTGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATc < 1:3310097/71‑1 (MQ=255) gtgtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtcat > 1:3271657/1‑40 (MQ=255) gtgtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtcat > 1:2341220/1‑40 (MQ=255) gtgtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtcat > 1:3767032/1‑40 (MQ=255) gtgtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGaa > 1:1329143/1‑45 (MQ=255) gtgtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATc < 1:1438624/71‑1 (MQ=255) gtgtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATc < 1:3372983/71‑1 (MQ=255) tgtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtc < 1:5046609/37‑1 (MQ=255) gtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCaa > 1:5005747/1‑71 (MQ=255) tACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCaa < 1:2911981/70‑1 (MQ=255) tACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCaa < 1:4699225/70‑1 (MQ=255) tACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCaa < 1:4205545/70‑1 (MQ=255) | GGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAA > W3110S.gb/2002091‑2002221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |