| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,018,964 | G→A | 58.5% | A92T (GCG→ACG) | fliI → | flagellum‑specific ATP synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,018,964 | 0 | G | A | 58.5% | 16.9 / 49.5 | 41 | A92T (GCG→ACG) | fliI | flagellum‑specific ATP synthase |
| Reads supporting (aligned to +/- strand): ref base G (11/6); new base A (11/13); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
GTTGGCTTTAACGGTCAACGGCTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCG > W3110S.gb/2018898‑2019027 | gTTGGCTTTAACGGTCAACGGCTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGcgcgcg > 1:1328968/1‑71 (MQ=255) gCTTTAACGGTCAACGGCTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCGCGCGTGtt < 1:4764689/71‑1 (MQ=255) ttAACGGTCAACGGCTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCGCGGCGCGCGTGTTTAt > 1:4614691/1‑71 (MQ=255) tAACGGTCAACGGCTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGAGCGCGTGTTTa > 1:4326103/1‑69 (MQ=255) ggTCAACGGCTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCACGCgtgt > 1:3689941/1‑62 (MQ=255) ggTCAACGGCTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCACGCGTGtt > 1:4570966/1‑63 (MQ=255) ggTCAACGGCTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCACGCGTGtt > 1:2315053/1‑63 (MQ=255) gTCAACGGCTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCACGCGTGTTTATGCCaaa < 1:3460278/71‑1 (MQ=255) gTCAACGGCTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCACGCGTGTTTATGCCaaa < 1:3267434/71‑1 (MQ=255) aaCGGCTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAAc < 1:309118/71‑1 (MQ=255) aaCGGCTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCACGCGTGTTTATGCCaaaaa < 1:1871949/70‑1 (MQ=255) cGGCTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCACGCGTGTTTATGCCAAAAACAt < 1:3481305/71‑1 (MQ=255) cGGCTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCACGCGTGTTTATGCCAAAAACAt < 1:2216446/71‑1 (MQ=255) tttttAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGgcacgc < 1:1279793/46‑1 (MQ=255) tttAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCgg > 1:4718433/1‑68 (MQ=255) tttAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCgg > 1:4658708/1‑68 (MQ=255) tttAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCgg > 1:1311980/1‑68 (MQ=255) tttAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCgg > 1:3255338/1‑68 (MQ=255) tttAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCgg > 1:3615569/1‑68 (MQ=255) tttAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCACGCgt > 1:4431467/1‑46 (MQ=255) aTGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCACGCGTGTTTATGCCAAAAACATTTCGGCAGaa > 1:2838721/1‑69 (MQ=255) ccGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCACGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGc < 1:1893183/70‑1 (MQ=255) ccGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCACGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCt > 1:1529033/1‑71 (MQ=255) ggaAGTCGAAGGTGTCCTGCCCGGCACGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCg > 1:4256109/1‑71 (MQ=255) aaGTCGAAGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGaa > 1:252084/1‑56 (MQ=255) aGTCGAAGGTGTCCTGCCCGGCACGCGTGTTTATGCCAAAAACATTTc > 1:1981955/1‑48 (MQ=255) gAAGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACAttt > 1:2175006/1‑43 (MQ=255) aaGGTGTCCTGCCCGGCACGCGTGTTTATGCCAAAAACATTTCGGCAg < 1:3493957/48‑1 (MQ=255) aaGGTGTCCTGCCCGGCACGCGTGTTTATGCCAAAAACATTTCGGCAg < 1:232238/48‑1 (MQ=255) aaGGTGTCCTGCCCGGCACGCGTGTTTATGCCAAAAACATTTCGGCAg < 1:4667183/48‑1 (MQ=255) aaGGTGTCCTGCCCGGCACGCGTGTTTATGCCAAAAACATTTCGGCAg < 1:4319929/48‑1 (MQ=255) ggTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAg < 1:1998895/46‑1 (MQ=255) gtgtCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCa < 1:3946430/67‑1 (MQ=255) gtgtCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCa < 1:222071/67‑1 (MQ=255) gtgtCCTGCCCGGCACGCGTGTTTATGCCAAAAACAtt < 1:948169/38‑1 (MQ=255) tCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCa < 1:3427556/64‑1 (MQ=255) cTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCaa > 1:3886959/1‑51 (MQ=255) cTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCaa > 1:2584697/1‑51 (MQ=255) cTGCCCGGCACGCGTGTTTATGCCAAAAACATTTCGGc > 1:3470733/1‑38 (MQ=255) cTGCCCGGCACGCGTGTTTATGCCAAAAACATTTCGGCa > 1:3248494/1‑39 (MQ=255) cccGGCACGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCa < 1:13695/59‑1 (MQ=255) cccGGCACGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAg > 1:1111877/1‑60 (MQ=255) cccGGCACGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCg < 1:3874194/70‑1 (MQ=255) | GTTGGCTTTAACGGTCAACGGCTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCG > W3110S.gb/2018898‑2019027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |