| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,021,281 | C→A | 43.5% | R253R (CGC→CGA) | fliK → | flagellar hook‑length control protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,021,281 | 0 | C | A | 43.5% | 72.3 / 60.7 | 62 | R253R (CGC→CGA) | fliK | flagellar hook‑length control protein |
| Reads supporting (aligned to +/- strand): ref base C (25/10); new base A (12/15); total (37/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.97e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTGAGTGCACCGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGTGCAAATCTCCCTCA > W3110S.gb/2021211‑2021348 | ttttGAGTGCACCGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGc < 1:1661460/71‑1 (MQ=255) ttGAGTGCACCGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGc > 1:561195/1‑69 (MQ=255) ttGAGTGCACCGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGc > 1:4826155/1‑69 (MQ=255) ttGAGTGCACCGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGc > 1:4168907/1‑69 (MQ=255) gAGTGCACCGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAg < 1:256182/70‑1 (MQ=255) gAGTGCACCGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAg < 1:4953119/70‑1 (MQ=255) ccGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAg > 1:3977129/1‑63 (MQ=255) ccGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAg > 1:1804495/1‑63 (MQ=255) ccGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGACAGGGGcaaca < 1:3843056/71‑1 (MQ=255) cGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGcaacaa < 1:3932515/71‑1 (MQ=255) cGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGACAGGGGcaaca > 1:206433/1‑70 (MQ=255) cGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGACAGGGGcaac > 1:1403391/1‑69 (MQ=255) cGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACACGCCAGGGGcaaca > 1:2981722/1‑70 (MQ=255) gCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGCGGCAACaaa < 1:762156/71‑1 (MQ=255) cTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACaaa < 1:2319085/70‑1 (MQ=255) ggTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGACAGGGGCAACAAAGTg > 1:3108547/1‑70 (MQ=255) ggTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGACAGGGGCAACAAAGTg > 1:277065/1‑70 (MQ=255) ggTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGACAGGGGCAACAAAGTg > 1:2487668/1‑70 (MQ=255) ttCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTg > 1:3232146/1‑68 (MQ=255) ttCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGACAGGGGCAACAAAGTGCag < 1:4792362/71‑1 (MQ=255) ttCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGACAGGGGCAACAAAGTGCag < 1:4025817/71‑1 (MQ=255) ctcACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCagag > 1:2275644/1‑71 (MQ=255) ctcACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCagag > 1:520427/1‑71 (MQ=255) ctcACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGACAGGGGCAACAAAGTGCagag > 1:2495781/1‑71 (MQ=255) ctcACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGACAGGGGCAACAAAGTGCagag > 1:3230181/1‑71 (MQ=255) tcACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGACAGGGGCAACAAAGTGCag < 1:2376532/68‑1 (MQ=255) aCGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGcaac < 1:2950661/57‑1 (MQ=255) aCGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGtt > 1:746307/1‑70 (MQ=255) aCGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGt < 1:5126307/69‑1 (MQ=255) gAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGcaacaa > 1:156304/1‑57 (MQ=255) gAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGcaacaa > 1:1983287/1‑57 (MQ=255) gAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGACAGGGGCAACAAAGTGCAGAGTTGCg < 1:2846381/71‑1 (MQ=255) acaaTCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCAcc > 1:1090187/1‑71 (MQ=255) acaaTCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCAcc > 1:2083001/1‑71 (MQ=255) acaaTCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCAcc > 1:1101366/1‑71 (MQ=255) caaTCATTATGCCAGCATATTTCGCTGTTCACCCGCCAGGGGcaaca < 1:3735534/47‑1 (MQ=255) caaTCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGcaaca < 1:2613132/47‑1 (MQ=255) aTCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCg > 1:53781/1‑70 (MQ=255) aTCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCg > 1:1448836/1‑70 (MQ=255) aTCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCg > 1:698443/1‑70 (MQ=255) tCATTAAGCCAGCATATTTAGCTGTTCACCCGACAGGGGcaaca < 1:4847527/44‑1 (MQ=255) aTTAAGCCAGCATATTTCGCTGTTCACCCGCCAgg > 1:3459010/1‑35 (MQ=255) aTTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACaaa > 1:4831484/1‑44 (MQ=255) aTTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACaaa > 1:1111056/1‑44 (MQ=255) aTTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGt > 1:4887262/1‑58 (MQ=255) aTTAAGCCAGCATATTTCGCTGTTCACCCGACAGGGGCAACAAAGTg < 1:3299722/47‑1 (MQ=255) tAAGCCAGCATATTTCGCTGTTCACCCGACAGGGGCAACtaa > 1:2089546/1‑42 (MQ=38) tAAGCCAGCATATTTCGCTGTTCACCCGACAGGGGCAACaaa > 1:2606780/1‑42 (MQ=255) aGCATATTTCGCTGTTCACCCGACAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGt < 1:308384/71‑1 (MQ=255) gCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGt > 1:3637427/1‑70 (MQ=255) cATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCaga > 1:320027/1‑41 (MQ=255) atatTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGt > 1:3042790/1‑47 (MQ=255) tatTTCGCTGTTCACCCGACAGGGGCAACAAAGTGCa < 1:1968632/37‑1 (MQ=255) tatTTCGCTGTTCACCCGACAGGGGCAACAAAGTGCa < 1:3266119/37‑1 (MQ=255) tatTTCGCTGTTCACCCGACAGGGGCAACAAAGTGCa < 1:2842877/37‑1 (MQ=255) tatTTCGCTGTTCACCCGACAGGGGCAACAAAGTGCa < 1:1947326/37‑1 (MQ=255) tatTTCGCTGTTCACCCGACAGGGGCAACAAAGTGCAGAGTTGCGt < 1:2062904/46‑1 (MQ=255) tatTTCGCTGTTCACCCGACAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGc < 1:1034013/56‑1 (MQ=255) tatTTCGCTGTTCACCCGACAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGc < 1:1956320/56‑1 (MQ=255) tttCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGTg > 1:1807731/1‑71 (MQ=255) tttCGCTGTTCACCCGACAGGGGCAACAAAGTGCAGAGTTGCGTCt > 1:3433849/1‑46 (MQ=255) tttCGCTGTTCACCCGACAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGt > 1:202760/1‑70 (MQ=255) ttCGCTGTTCACCCGCCAGGGGCAACAAAGTTCAGAGTTGCGTCTGc < 1:3065202/47‑1 (MQ=255) gCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGTGCaaa > 1:5061820/1‑71 (MQ=255) cACCCGACAGGTGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGTGCAAATCTccc > 1:4190731/1‑71 (MQ=255) cGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGTGCAAATCTCCCTCa > 1:2345650/1‑70 (MQ=255) | TTTTGAGTGCACCGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGTGCAAATCTCCCTCA > W3110S.gb/2021211‑2021348 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |