| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,055,031 | C→T | 5.8% | pseudogene (435/1053 nt) pseudogene (435/705 nt) |
yeeL ← yeeL ← |
ECK1975:JW1961+JW5325:b4497; hypothetical protein ECK1975:JW1961:b1980; hypothetical protein, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,055,031 | 0 | C | T | 5.8% | 225.9 / 3.0 | 69 | pseudogene (435/1053 nt) pseudogene (435/705 nt) | yeeL yeeL | ECK1975:JW1961+JW5325:b4497; hypothetical protein ECK1975:JW1961:b1980; hypothetical protein, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base C (29/36); new base T (2/2); total (31/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
ATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGT > W3110S.gb/2054963‑2055101 | aTAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCAt > 1:879212/1‑71 (MQ=255)aTAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCAt > 1:2439838/1‑71 (MQ=255) ggTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGt < 1:1233247/71‑1 (MQ=255) ggTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGt < 1:5112032/71‑1 (MQ=255) gCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACcaac < 1:4156195/71‑1 (MQ=255) aGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCt > 1:448017/1‑70 (MQ=255) tttCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCAt < 1:294216/51‑1 (MQ=255) tttCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCAt < 1:5108292/51‑1 (MQ=255) tCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGaaaa > 1:2900678/1‑71 (MQ=255) tCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGaaaa > 1:342611/1‑71 (MQ=255) tCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGaaa < 1:1249305/70‑1 (MQ=255) aTCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTTATGGTTATACca < 1:2686254/55‑1 (MQ=255) aTCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTTATGGTTATACca < 1:1274610/55‑1 (MQ=255) aTCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAAt < 1:4934033/68‑1 (MQ=255) aTCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAAt < 1:3020897/68‑1 (MQ=255) aTCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAAt < 1:2981828/68‑1 (MQ=255) tCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGt < 1:2620918/71‑1 (MQ=255) tACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGttttt > 1:4694381/1‑71 (MQ=255) tACTGGACGCGTGCTTGCACAAAGGGGAGAAGCAATTCATGGt < 1:4626606/43‑1 (MQ=255) cTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACcaa > 1:4518704/1‑49 (MQ=255) cTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGc > 1:1100120/1‑71 (MQ=255) gACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAAtt > 1:2088545/1‑59 (MQ=255) gACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCtt < 1:2544802/70‑1 (MQ=255) gACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCtt < 1:803582/70‑1 (MQ=255) gACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCtt < 1:4848683/70‑1 (MQ=255) aCGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTc < 1:4164884/71‑1 (MQ=255) aCGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTc < 1:4794113/71‑1 (MQ=255) aCGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTc < 1:1283820/71‑1 (MQ=255) gcgTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGttttt > 1:5061961/1‑63 (MQ=255) gTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGttt > 1:4136554/1‑59 (MQ=255) gTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAact < 1:4550933/71‑1 (MQ=255) gTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAact < 1:1862083/71‑1 (MQ=255) ttCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTc < 1:4947768/66‑1 (MQ=255) ttCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAactg < 1:699063/71‑1 (MQ=255) ttCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAact < 1:4043644/70‑1 (MQ=255) cTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGtttt > 1:2225525/1‑57 (MQ=255) cTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGtttt > 1:4840918/1‑57 (MQ=255) cTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGt < 1:3003176/54‑1 (MQ=255) cTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGt < 1:1469736/54‑1 (MQ=255) cTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAactgac > 1:4116160/1‑71 (MQ=255) cTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAactgac > 1:1914874/1‑71 (MQ=255) cTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAactgac > 1:4421976/1‑71 (MQ=255) cTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAactgac > 1:2697117/1‑71 (MQ=255) cTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACATGAAAATTCGTTTTTGCTTTCAactgac > 1:4612816/1‑71 (MQ=255) cTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAA‑AACCTGAAAATTCGTTTTTGCTTTCAactga > 1:4371702/1‑69 (MQ=255) cacaAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGat < 1:2819130/71‑1 (MQ=255) acaAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAactgact > 1:3480530/1‑67 (MQ=255) acaAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGat > 1:1679899/1‑70 (MQ=255) caAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGaa < 1:3307529/41‑1 (MQ=255) aGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGataaata < 1:1929511/71‑1 (MQ=255) gggAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGtttt < 1:4316793/46‑1 (MQ=255) gggAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGtttt < 1:2945065/46‑1 (MQ=255) gagaAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACAt > 1:1242320/1‑71 (MQ=255) gagaAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGATTTCAACTGACTGATAAATAACa > 1:1031435/1‑70 (MQ=255) gaAGCAATTTATGGTTATACCAACAACCTGAAAATTCGttttt > 1:5031304/1‑43 (MQ=255) gaAGCAATTTATGGTTATACCAACAACCTGAAAATTCGttttt > 1:3799482/1‑43 (MQ=255) gaAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGataaataa > 1:1251407/1‑66 (MQ=255) aGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGtt < 1:4945920/71‑1 (MQ=255) gCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGttt > 1:2879545/1‑71 (MQ=255) gCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGttt > 1:1805521/1‑71 (MQ=255) cAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTg > 1:900397/1‑40 (MQ=255) cAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTg > 1:1931421/1‑40 (MQ=255) cAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTg > 1:3384390/1‑40 (MQ=255) ttCATGGTTATACCAACAACCTGAAAATTCGttttt < 1:3363375/36‑1 (MQ=255) ttCATGGTTATACCAACAACCTGAAAATTCGtttt < 1:2295778/35‑1 (MQ=255) ttCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTc < 1:4894020/42‑1 (MQ=255) ttCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAact < 1:3216858/46‑1 (MQ=255) ttCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCa < 1:4662973/70‑1 (MQ=255) ttCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCa < 1:4615320/70‑1 (MQ=255) cATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTc < 1:4008259/40‑1 (MQ=255) cATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCa > 1:3117843/1‑41 (MQ=255) cATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAactga > 1:4664881/1‑46 (MQ=255) cATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAactga > 1:4490777/1‑46 (MQ=255) cATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGt > 1:2785005/1‑71 (MQ=255) cATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGt > 1:4318538/1‑71 (MQ=255) | ATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGT > W3110S.gb/2054963‑2055101 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |