| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,099,387 | T→C | 23.3% | intergenic (+25/+71) | hisI → / ← cld | fused phosphoribosyl‑AMP cyclohydrolase and phosphoribosyl‑ATP pyrophosphatase/regulator of length of O‑antigen component of lipopolysaccharide chains |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,099,387 | 0 | T | C | 23.3% | 53.0 / 15.1 | 30 | intergenic (+25/+71) | hisI/cld | fused phosphoribosyl‑AMP cyclohydrolase and phosphoribosyl‑ATP pyrophosphatase/regulator of length of O‑antigen component of lipopolysaccharide chains |
| Reads supporting (aligned to +/- strand): ref base T (7/16); new base C (3/4); total (10/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATTTAACGACGGTAATTGAGAACCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCAAAAAAACGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAA > W3110S.gb/2099318‑2099448 | gATTTAACGACGGTAATTGAGAACCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGatat < 1:3021996/70‑1 (MQ=255)gATTTAACGACGGTAATTGAGAACCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGatat > 1:3291105/1‑70 (MQ=255)gATTTAACGACGGTAATTGAGAACCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATAtt > 1:3744326/1‑71 (MQ=255)gATTTAACGACGGTAATTGAGAACCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATAtt > 1:856803/1‑71 (MQ=255) tttAACGACGGTAATTGAGAACCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATAttgt < 1:1216663/71‑1 (MQ=255) tAACGACGGTAATTGAGAACCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATAttgttg > 1:4772549/1‑71 (MQ=255) tAACGACGGTAATTGAGAACCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATAttgttg > 1:4178111/1‑71 (MQ=255) tAACGACGGTAATTGAGAACCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATAttgtt < 1:218491/70‑1 (MQ=255) ggTAATTGAGAACCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGatat > 1:933294/1‑59 (MQ=255) ggTAATTGAGAACCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGatat > 1:4612544/1‑59 (MQ=255) gTATTTGAGTACCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATcc < 1:2553648/71‑1 (MQ=255) gTAATTGAGAACCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATcc < 1:2876039/71‑1 (MQ=255) gTAATTGAGAACCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATcc < 1:2886129/71‑1 (MQ=255) ttGAGAACCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCa < 1:3518067/71‑1 (MQ=255) aaCCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCaaaaaa < 1:2360437/71‑1 (MQ=255) aCCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCaaaaaaa < 1:2169228/71‑1 (MQ=255) ccTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCAAAAAAAc < 1:4202998/71‑1 (MQ=255) ccTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCAAAAAAAc < 1:1364849/71‑1 (MQ=255) cTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGatat < 1:812770/46‑1 (MQ=255) cTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGatat < 1:4811064/46‑1 (MQ=255) tGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATcc < 1:3959197/58‑1 (MQ=255) gCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCaaaaaaa > 1:2217438/1‑67 (MQ=255) cGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCAAAAAAACGGGc < 1:1095618/71‑1 (MQ=255) tAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCaaaaaaa < 1:2824340/64‑1 (MQ=255) tAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCAAAAAAACGGGCaa < 1:538630/71‑1 (MQ=255) tAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCAAAAAAACGGGCaa < 1:1125976/71‑1 (MQ=255) aaCGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCAAAAAAACGGGCAAg < 1:2988880/70‑1 (MQ=255) aaCGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCAAAAAAACGGGCAAg < 1:1029061/70‑1 (MQ=255) aaCGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATACTGTTGCCGCATCCGGCAAAAAAACGGGCAAgg < 1:3787343/71‑1 (MQ=255) ggCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCAAAAAAACGGGCAAGgtgt < 1:2954004/71‑1 (MQ=255) tCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCAAAAAAACGGGCAAGGTGTcac > 1:1658307/1‑70 (MQ=255) aGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCAAAAAAACGGGCAAGGTGTcaccac > 1:4388765/1‑71 (MQ=255) tGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCAAAAAAACGGGCAAGGTGTCACCAccc > 1:2948148/1‑71 (MQ=255) aGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCAAAAAAACGGGCAAGgt > 1:2364199/1‑59 (MQ=255) gCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCAAAAAAACGGGCAAGGTGTCACCACCCTGCCCt > 1:4197454/1‑71 (MQ=255) tAAGCGGATGCGATACTGTTGCCGCATCCGGCAAAAAAACGGGCaa < 1:4316028/46‑1 (MQ=255) tAAGCGGATGCGATACTGTTGCCGCATCCGGCAAAAAAACGGGCaa < 1:3859059/46‑1 (MQ=255) aGCGGATGCGATACTGTTGCCGCATCCGGCAAAAAAACGGGCaa > 1:4253028/1‑44 (MQ=255) aGCGGATGCGATACTGTTGCCGCATCCGGCAAAAAAACGGGCaa > 1:933511/1‑44 (MQ=255) aGCGGATGCGATACTGTTGCCGCATCCGGCAAAAAAACGGGCAAGGTGTCaccacc > 1:736317/1‑56 (MQ=255) ggATGCGATACTGTTGCCGCATCCGGCAAAAAAACGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTaa < 1:3982776/70‑1 (MQ=255) gATGCGATATTGTTGCCGCATCCGGCAAAAAAACGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTaaaa > 1:3816893/1‑71 (MQ=255) atTGTTGCCGCATCCGGCAAAAAAACGGGCAAGgtgt < 1:5059813/37‑1 (MQ=255) atTGTTGCCGCATCCGGCAAAAAAACGGGCAAGGTGTcac < 1:857344/40‑1 (MQ=255) | GATTTAACGACGGTAATTGAGAACCTGCGTAAACGGCATCAGTGAGTTGCGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCAAAAAAACGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAA > W3110S.gb/2099318‑2099448 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |