| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,170,757 | T→C | 7.9% | intergenic (‑314/‑92) | yegR ← / → yegS | hypothetical protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,170,757 | 0 | T | C | 7.9% | 248.0 / 11.7 | 88 | intergenic (‑314/‑92) | yegR/yegS | hypothetical protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (48/33); new base C (4/3); total (52/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
GTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTACACTATCTACAGAC > W3110S.gb/2170689‑2170825 | gTTAGTGTTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACttt > 1:4235382/1‑71 (MQ=255)gTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACttt > 1:454687/1‑71 (MQ=255) tAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACtttt > 1:3953840/1‑70 (MQ=255) aGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTa > 1:268458/1‑71 (MQ=255) tATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTa < 1:1968579/69‑1 (MQ=255) ttCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTAtcct > 1:1858979/1‑71 (MQ=255) ttCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTAtcc < 1:77056/70‑1 (MQ=255) tCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTAtcctc > 1:4471658/1‑71 (MQ=255) gTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACtttt < 1:4398540/59‑1 (MQ=255) gTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACtttt < 1:5095303/59‑1 (MQ=255) tCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGAc > 1:4087413/1‑69 (MQ=255) ccATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCa < 1:2899153/71‑1 (MQ=255) ttGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATcc < 1:1034885/71‑1 (MQ=255) ttGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATcc < 1:3292816/71‑1 (MQ=255) ttGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATcc > 1:3057223/1‑71 (MQ=255) ttGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATcc < 1:1511386/71‑1 (MQ=255) gCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTg > 1:3729032/1‑71 (MQ=255) aGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTc < 1:4465994/71‑1 (MQ=255) aGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTc > 1:1381208/1‑71 (MQ=255) gCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCt < 1:4440553/71‑1 (MQ=255) gCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCt < 1:604601/71‑1 (MQ=255) gCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCt < 1:2813317/71‑1 (MQ=255) cTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACCTTTTATCCTCCGACtt > 1:4625892/1‑58 (MQ=255) cTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACCTTTTATCCTCCGACTTTCATCCTGtt > 1:3463996/1‑68 (MQ=255) cTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACCTTTTATCCTCCGACTTTCATCCTGtt > 1:809733/1‑68 (MQ=255) cTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACCTTTTATCCTCCGACTTTCATCCTGtt > 1:3797523/1‑68 (MQ=255) cTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGa < 1:2711003/69‑1 (MQ=255) cTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGAc < 1:374707/70‑1 (MQ=255) ggtggtAACCGCTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGAcag > 1:4000385/1‑68 (MQ=255) ggtggtAACCACTGAATCCTCCATACTTGAACTGACttt > 1:2193246/1‑39 (MQ=255) ggtggtAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTc > 1:2611679/1‑70 (MQ=255) ggtggtAACCACTGAATCCTACATACTTGAACTGACttt > 1:321052/1‑39 (MQ=255) gtggtAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCa > 1:291086/1‑71 (MQ=255) gtggtAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCa > 1:3657994/1‑71 (MQ=255) ggtAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCAcc > 1:3987282/1‑71 (MQ=255) gtAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCt > 1:490220/1‑71 (MQ=255) gtAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCt > 1:4606237/1‑71 (MQ=255) tAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCtt > 1:2791043/1‑71 (MQ=255) tAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCtt > 1:202654/1‑71 (MQ=255) tAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCtt > 1:4384172/1‑71 (MQ=255) aaCCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCttt > 1:786224/1‑71 (MQ=255) aCTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGa > 1:3264170/1‑57 (MQ=255) aCTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGa > 1:557141/1‑57 (MQ=255) aCTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGtt < 1:4792144/71‑1 (MQ=255) cTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGAc < 1:3471843/57‑1 (MQ=255) gAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGt > 1:3584560/1‑49 (MQ=255) tcctccATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTg < 1:3405159/45‑1 (MQ=255) tcctccATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGc < 1:2599062/71‑1 (MQ=255) tcctccATACTTGAACTGACCTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTc < 1:4606720/68‑1 (MQ=255) tcctccATACTTGAACTGACCTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTc < 1:3537362/68‑1 (MQ=255) tcctccATACTTGAACTGACCTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTc < 1:1945575/68‑1 (MQ=255) ccATTCTTGATCTGACTTTTTATCCTCCGACTTTCATCCTGTTCt < 1:4058001/45‑1 (MQ=38) ccATACTTGAACTGACTTTTTATCCTCCGACTTTCATc > 1:2191253/1‑38 (MQ=255) ccATACTTGAACTGACTTTTTATCCTCCGACTTTCATc > 1:4979851/1‑38 (MQ=255) ccATACTTGAACTGACTTTTTATCCTCCGACTTTCATc > 1:674681/1‑38 (MQ=255) ccATACTTGAACTGACTTTTTATCCTCCGACTTTCATc > 1:4084864/1‑38 (MQ=255) ccATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCAc > 1:930349/1‑53 (MQ=255) ccATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCAc > 1:1890700/1‑53 (MQ=255) cATACTTGAACTGACTTTTTATCCTCCGACTTTCTTCCTGTTGTGACTCCACCTTTTGtt < 1:2114107/60‑1 (MQ=255) cATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGctct < 1:3850296/69‑1 (MQ=255) cATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTac < 1:4276465/71‑1 (MQ=255) cATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTa > 1:4959953/1‑70 (MQ=255) cATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTa > 1:1391329/1‑70 (MQ=255) aTACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGtt < 1:4904098/59‑1 (MQ=255) aTACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGtt < 1:625575/59‑1 (MQ=255) aTACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGtt < 1:4223881/59‑1 (MQ=255) tACTTGAACTGACTTTTTATCCTCCGACTTTCATCTTGTTCt < 1:5532/42‑1 (MQ=255) tACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTg < 1:4075866/38‑1 (MQ=255) tACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTg < 1:935652/38‑1 (MQ=255) tACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCt < 1:4107513/42‑1 (MQ=255) tACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTacac < 1:714586/71‑1 (MQ=255) tACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTaca > 1:3417297/1‑70 (MQ=255) aCTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCtt > 1:1059397/1‑52 (MQ=255) cTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGtt > 1:3266352/1‑38 (MQ=255) cTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGtt < 1:5019968/56‑1 (MQ=255) cTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTc > 1:4710885/1‑59 (MQ=255) cTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTACACTa > 1:4957366/1‑71 (MQ=255) cTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTACACTa > 1:2190929/1‑71 (MQ=255) tGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCttt > 1:3710815/1‑50 (MQ=255) tGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGtttt > 1:1371865/1‑56 (MQ=255) tGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTACACTATc > 1:1981232/1‑71 (MQ=255) tGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTATCTGCTCTACACTAt > 1:4052533/1‑70 (MQ=255) gAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGa > 1:1856998/1‑39 (MQ=255) gAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGa > 1:3511481/1‑39 (MQ=255) aaCTGACTTTTTATCCTCCGACTTTCATCCTGTTCt < 1:3081096/36‑1 (MQ=255) aCTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTACACTATCTa > 1:3311711/1‑70 (MQ=255) aCTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTACACTATCTa > 1:2677726/1‑70 (MQ=255) cTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCa < 1:1224560/41‑1 (MQ=255) cTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTa > 1:5127760/1‑60 (MQ=255) cTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTa > 1:2999414/1‑60 (MQ=255) cTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTACACTATCTACa < 1:3530522/71‑1 (MQ=255) cTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTACACTATCTACa < 1:1181516/71‑1 (MQ=255) gACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTACACTATCTACAGa > 1:3705537/1‑71 (MQ=255) aCTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTg > 1:2542719/1‑52 (MQ=255) aCTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTACACTATCTACAGAc > 1:226493/1‑71 (MQ=255) tttttATCCTCCGACTTTCATCCTGTTCTGACTCCa > 1:4572335/1‑36 (MQ=255) tttttATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTACACTATCTACa < 1:3762156/66‑1 (MQ=255) | GTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTACACTATCTACAGAC > W3110S.gb/2170689‑2170825 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |