| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,214 | T→C | 51.3% | F276F (TTT→TTC) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,214 | 0 | T | C | 51.3% | 5.6 / 115.6 | 78 | F276F (TTT→TTC) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (19/19); new base C (19/21); total (38/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
GTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAA > W3110S.gb/71147‑71284 | gTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTc > 1:916765/1‑69 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGc > 1:3295469/1‑70 (MQ=255) ttGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGcc > 1:124252/1‑71 (MQ=255) tGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCAAGCCCGAGCGAGTTTCGTGc < 1:3806366/69‑1 (MQ=255) gATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtg < 1:4419078/71‑1 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg < 1:3854123/70‑1 (MQ=255) cAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTtgtg > 1:1963803/1‑70 (MQ=255) tATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaa > 1:4364241/1‑70 (MQ=255) aTTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttt < 1:4595894/50‑1 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaaaa < 1:298711/70‑1 (MQ=255) ttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTtgt < 1:1103441/60‑1 (MQ=255) ttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTtgt < 1:3043087/60‑1 (MQ=255) tctcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGt > 1:3028893/1‑70 (MQ=255) ctcGCGAGTATTTAAAAAATGCACCGGGGCCCGCCCGAGCGAGTTTc > 1:1290516/1‑47 (MQ=255) ctcGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTc > 1:2421730/1‑47 (MQ=255) gcgAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGa < 1:3685429/68‑1 (MQ=255) gcgAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:5034420/71‑1 (MQ=255) gcgAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAat < 1:2446440/70‑1 (MQ=255) cgggTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatga < 1:3142294/68‑1 (MQ=255) cgggTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatga < 1:1156611/68‑1 (MQ=255) cgggTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatga < 1:1031409/68‑1 (MQ=255) gggTAATTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatgat > 1:1170139/3‑71 (MQ=255) aGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTgaaga < 1:1170574/57‑1 (MQ=255) tATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTtg < 1:2486722/49‑1 (MQ=255) tATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTa < 1:3967646/71‑1 (MQ=255) tttAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCg > 1:4603831/1‑38 (MQ=255) ttAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGcc > 1:246939/1‑71 (MQ=255) ttAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGcc > 1:4928152/1‑71 (MQ=255) ttAAAAAATGCACCGGGGCCAGCCCCAGCGAGTTCCGTGCCGGTTGTGAAGaaaaa < 1:4622507/56‑1 (MQ=39) tAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGt > 1:4981909/1‑37 (MQ=255) tAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGt > 1:2007993/1‑37 (MQ=255) tAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGcc < 1:3293780/70‑1 (MQ=255) aaaaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTTCCGGTtg < 1:210535/42‑1 (MQ=38) aaTGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAg < 1:873939/71‑1 (MQ=255) aaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTTAAGaaaaa < 1:4061797/50‑1 (MQ=255) aaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAg < 1:4177407/71‑1 (MQ=255) aaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAg < 1:2131866/71‑1 (MQ=255) cACCGGGGCCAGCCCGAGCGAGTTCCGTGCCTGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTg < 1:273899/70‑1 (MQ=255) cACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTg < 1:4270140/70‑1 (MQ=255) aCCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTgaagaa < 1:115526/42‑1 (MQ=255) aCCGGGGCCAGCCCGAGCGAATTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGt > 1:2495982/1‑70 (MQ=255) ccGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTg < 1:3468054/47‑1 (MQ=255) ccGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTg < 1:4215795/47‑1 (MQ=255) ccGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTc > 1:1629056/1‑70 (MQ=255) ccGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTc > 1:2237057/1‑70 (MQ=255) cGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCAt < 1:1907144/71‑1 (MQ=255) cGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:202980/41‑1 (MQ=255) cGGGGCCAGCCCGAGAGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGt < 1:671108/54‑1 (MQ=255) ggggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGtt > 1:1232059/1‑65 (MQ=255) ggggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGtt > 1:2514239/1‑65 (MQ=255) ggggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGtt > 1:4160980/1‑65 (MQ=255) ggggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGtt > 1:231054/1‑65 (MQ=255) gggCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaa < 1:2493709/39‑1 (MQ=255) gggCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATaa > 1:4895864/1‑71 (MQ=255) gggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATa > 1:1735312/1‑70 (MQ=255) gCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAtt < 1:4622061/71‑1 (MQ=255) aGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTgg > 1:984522/1‑70 (MQ=255) aGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGt < 1:4014367/71‑1 (MQ=255) aGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGa > 1:160507/1‑40 (MQ=255) aGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatga < 1:4757284/44‑1 (MQ=255) gCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTa < 1:2023206/71‑1 (MQ=255) ccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGa > 1:1175031/1‑37 (MQ=255) ccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGa > 1:4281654/1‑37 (MQ=255) ccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGtt > 1:5017766/1‑56 (MQ=255) ccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccc > 1:933518‑M1/1‑63 (MQ=255) ccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccc > 1:2926905‑M1/1‑63 (MQ=255) cgagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGt > 1:3937332/1‑43 (MQ=255) cgagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAAc > 1:2368014/1‑70 (MQ=255) cgagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAAc > 1:217381/1‑70 (MQ=255) cgagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAAc > 1:1203726/1‑70 (MQ=255) agcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGaa < 1:3044517/71‑1 (MQ=255) agcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGt > 1:1785979/1‑41 (MQ=255) cgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccctaagt < 1:5084438‑M1/71‑14 (MQ=255) cgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccctaagt < 1:3462520‑M1/71‑14 (MQ=255) gTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGt < 1:5127305/50‑1 (MQ=255) gTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGt < 1:1162239/50‑1 (MQ=255) gTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGa > 1:1509290/1‑71 (MQ=255) gTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGa > 1:3443990/1‑71 (MQ=255) gTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGa > 1:4097798/1‑71 (MQ=255) ttCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGAc < 1:1174438/70‑1 (MQ=255) tCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAg > 1:4780985/1‑43 (MQ=255) tCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGa > 1:1359347/1‑68 (MQ=255) tCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGa > 1:1177540/1‑68 (MQ=255) tCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGa > 1:4193070/1‑68 (MQ=255) tCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACaa > 1:1395161/1‑71 (MQ=255) tCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACaa > 1:4766220/1‑71 (MQ=255) tCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACaa > 1:5092939/1‑71 (MQ=255) tCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACaa > 1:1871704/1‑71 (MQ=255) tCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACaa > 1:459521/1‑71 (MQ=255) tCGTGCAGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAg > 1:1299965/1‑43 (MQ=255) | GTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAA > W3110S.gb/71147‑71284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |