| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,176,396 | C→T | 35.6% | M6I (ATG→ATA) | gatC ← | galactitol‑specific enzyme IIC component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,176,396 | 0 | C | T | 35.6% | 76.8 / 72.1 | 73 | M6I (ATG→ATA) | gatC | galactitol‑specific enzyme IIC component of PTS |
| Reads supporting (aligned to +/- strand): ref base C (19/28); new base T (11/15); total (30/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 | |||||||||||
ATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGA > W3110S.gb/2176329‑2176466 | atatTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCAt < 1:1912960/70‑1 (MQ=255)atatTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCAt < 1:121350/70‑1 (MQ=255)atatTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCAt > 1:1658028/1‑70 (MQ=255) tatTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGa > 1:932376/1‑71 (MQ=255) tttAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACtt > 1:4198209/1‑71 (MQ=255) tttAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACtt > 1:4138633/1‑71 (MQ=255) tttAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACtt > 1:4683618/1‑71 (MQ=255) ttAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGc > 1:1716923/1‑63 (MQ=255) ttAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTc > 1:2996894/1‑71 (MQ=255) ttAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTc > 1:830954/1‑71 (MQ=255) ttAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGa > 1:4124433/1‑67 (MQ=255) ttAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACtt < 1:283355/70‑1 (MQ=255) ttAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACtt < 1:1684183/70‑1 (MQ=255) ttAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTc > 1:3717833/1‑71 (MQ=255) ttAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTc > 1:2214610/1‑71 (MQ=255) aataatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACa < 1:3491017/71‑1 (MQ=255) aataatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACa < 1:3701724/71‑1 (MQ=255) aataatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACa < 1:454736/71‑1 (MQ=255) aataatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCAt < 1:2786486/57‑1 (MQ=255) aataatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACa < 1:3472036/71‑1 (MQ=255) aataatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACa < 1:410335/71‑1 (MQ=255) aataatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACa < 1:4945574/71‑1 (MQ=255) ataatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACAt < 1:1039720/71‑1 (MQ=255) taatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATg < 1:763020/56‑1 (MQ=255) atgaCAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTc < 1:2890866/70‑1 (MQ=255) ggCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACATAGGTCACCCCTGTa < 1:503983/70‑1 (MQ=255) ggCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACATAGGTCACCCCTGTa < 1:3611034/70‑1 (MQ=255) ggCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTaa < 1:3368438/71‑1 (MQ=255) ggCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTaa < 1:3848716/71‑1 (MQ=255) ggCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTaa < 1:1474040/71‑1 (MQ=255) ggCAGCAACACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACATAGGTCAccc > 1:2618521/1‑65 (MQ=255) gcaTCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACAt > 1:1379374/1‑52 (MQ=255) gcaTCACCGTAGGGCCGAGGTCGAGAATATAACGCAt < 1:1104367/37‑1 (MQ=255) tCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTa < 1:2661938/63‑1 (MQ=255) tCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTa < 1:3939693/63‑1 (MQ=255) cACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGt > 1:4202570/1‑69 (MQ=255) aCCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGt > 1:4566554/1‑68 (MQ=255) aCCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAg < 1:1770363/71‑1 (MQ=255) aCCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAg < 1:1447794/71‑1 (MQ=255) ccGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAg > 1:3002486/1‑70 (MQ=255) cGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAgg > 1:4933566/1‑48 (MQ=255) cGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAgg > 1:1802229/1‑48 (MQ=255) cGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAgg > 1:4160581/1‑48 (MQ=255) gggCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAattt > 1:2743879/1‑70 (MQ=255) ggCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAatttt < 1:1396727/70‑1 (MQ=255) ggCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAatttta < 1:2579898/71‑1 (MQ=255) gCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGa < 1:1572020/64‑1 (MQ=255) ccGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCAcccc < 1:84203/48‑1 (MQ=255) gAGGTCGAGAATATAACGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAattttatttt < 1:1922480/71‑1 (MQ=255) gAGGTCGAGAATATAACGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAattttatttt < 1:4599358/71‑1 (MQ=255) aGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTg < 1:1103960/71‑1 (MQ=255) ggTCGAGAATATAACGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGt < 1:578080/71‑1 (MQ=255) ggTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTg < 1:2065154/70‑1 (MQ=255) ggTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTCTAAGATAGTCAGAATTTTATTTTGt < 1:4292732/71‑1 (MQ=255) gTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTg > 1:758058/1‑69 (MQ=255) tCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGa < 1:4614249/49‑1 (MQ=255) gaATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAattt > 1:2123936/1‑57 (MQ=255) gaATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAattt > 1:2364001/1‑57 (MQ=255) gaATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCt < 1:2393231/71‑1 (MQ=255) aaTATAACGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAattt < 1:4378243/56‑1 (MQ=255) aaTATAACGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAattt < 1:1798979/56‑1 (MQ=255) atatAACGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAattt < 1:2567630/55‑1 (MQ=255) atatAACGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTc < 1:2365397/71‑1 (MQ=255) tataACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAatttta < 1:4295962/56‑1 (MQ=255) tataACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAatttta < 1:1426107/56‑1 (MQ=255) ataACGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTcga > 1:4643712/1‑71 (MQ=255) taACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAg > 1:1067475/1‑43 (MQ=255) taACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAattttatt > 1:4623516/1‑56 (MQ=255) taACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAattttatt > 1:2362114/1‑56 (MQ=255) taACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAattttat > 1:3381206/1‑55 (MQ=255) aaCGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCt > 1:1961787/1‑65 (MQ=255) aCGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTaa > 1:91064/1‑60 (MQ=255) aCGCATGACTTCTGAAAACATAGGTCACCACTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTcgatac > 1:551769/1‑71 (MQ=255) gCATGACTTCTGAAAACATAGCTCACCCCTGTAAGATAGTCAGAATTTTATTTTGt < 1:3728709/56‑1 (MQ=255) cATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTcgataccga > 1:2687692/1‑71 (MQ=255) | ATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGA > W3110S.gb/2176329‑2176466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |