| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,218,928 | T→C | 10.5% | I243I (ATT→ATC) | mlrA → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,218,928 | 0 | T | C | 10.5% | 236.6 / 17.7 | 86 | I243I (ATT→ATC) | mlrA | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (43/34); new base C (5/4); total (48/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
GAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCG > W3110S.gb/2218858‑2218995 | gAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCAtt > 1:41772/1‑71 (MQ=255) ccGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGa > 1:882347/1‑71 (MQ=255) ccGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGa > 1:3274430/1‑71 (MQ=255) ccGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGa > 1:4531461/1‑71 (MQ=255) ccGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGa > 1:4981473/1‑71 (MQ=255) ccGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGa > 1:4975629/1‑71 (MQ=255) ccGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGa > 1:245237/1‑71 (MQ=255) cGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTaa > 1:2230111/1‑67 (MQ=255) cGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTaa > 1:3599700/1‑67 (MQ=255) cGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATg < 1:4866047/69‑1 (MQ=255) cGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGAt < 1:2439964/71‑1 (MQ=255) cGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGAt < 1:4435733/71‑1 (MQ=255) cGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGAt < 1:2215045/71‑1 (MQ=255) gCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGAtt < 1:69400/71‑1 (MQ=255) cAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGt < 1:1828716/71‑1 (MQ=255) cAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGt < 1:3899111/71‑1 (MQ=255) cAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATCTAATGATTCGt < 1:580078/71‑1 (MQ=255) gcagcaACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACa < 1:2574195/71‑1 (MQ=255) gcagcaACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACa < 1:4336191/71‑1 (MQ=255) cagcaACTCACCAGTTGGCAAGAACTAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACat < 1:1449553/71‑2 (MQ=255) cagcaACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACa < 1:4724723/70‑1 (MQ=255) aaCTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATgc < 1:2231381/71‑1 (MQ=255) cTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCg < 1:2679451/58‑1 (MQ=255) cTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTaa > 1:2914717/1‑62 (MQ=255) tCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCt > 1:4699089/1‑71 (MQ=255) tCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCt > 1:582838/1‑71 (MQ=255) tCACCAGTTGGAAAGAACAAGGCCATGATATTTTCCCACTCGGCATCTAATGATTCGTTAACAAATGCGCt > 1:2372578/1‑71 (MQ=255) cACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGAGCtt > 1:3821110/1‑71 (MQ=255) aacAGTTGGCAAGAACAAGGCCATGATATTTTCACACTCGGCATTTAATGAt > 1:1814399/3‑52 (MQ=255) aCCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCtt < 1:3892047/70‑1 (MQ=255) aCCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCtt < 1:769178/70‑1 (MQ=255) gTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTa < 1:1855403/43‑1 (MQ=255) gTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGtt < 1:3689134/53‑1 (MQ=255) ttGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGAtt < 1:1886418/48‑1 (MQ=255) ttGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACaa < 1:1594975/57‑1 (MQ=255) ttGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACa < 1:1962365/56‑1 (MQ=255) ttGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTg > 1:4586865/1‑70 (MQ=255) ttGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTg > 1:493559/1‑70 (MQ=255) tGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATCTAATGATTCGTTAACAAATGCGCTTTACTGTa > 1:4731388/1‑71 (MQ=255) cAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAAt < 1:225127/40‑1 (MQ=255) aGAACAAGGCCATGATATTTTCCCACTCGGCATTTAAt < 1:3293177/38‑1 (MQ=255) aGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGa > 1:1028665/1‑40 (MQ=255) aGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGa > 1:2171808/1‑40 (MQ=255) aGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGa > 1:1240896/1‑40 (MQ=255) aGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATc > 1:2001738/1‑71 (MQ=255) gAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATcc > 1:149409/1‑71 (MQ=255) gAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATcc > 1:3343609/1‑71 (MQ=255) aaCAAGGCCATGATATTTTCCCACTCGGCATTTAATGa > 1:1386522/1‑38 (MQ=255) aaCAAGGCCATGATATTTTCCCACTCGGCATTTAATGa > 1:2558069/1‑38 (MQ=255) aaCAAGGCCATGATATTTTCCCACTCGGCATTTAATGa > 1:1587300/1‑38 (MQ=255) aaCAAGGCCATGATATTTTCCCACTCGGCATTTAATGa > 1:1891390/1‑38 (MQ=255) aaCAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAAc < 1:7597/47‑1 (MQ=255) aCAAGGCCATGATATTTTCCCACTCGGCATTTAATg > 1:3221635/1‑36 (MQ=255) aCAAGGCCATGATATTTTCCCACTCGGCATTTAATg > 1:2186803/1‑36 (MQ=255) aCAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCtt < 1:2851586/71‑1 (MQ=255) cAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCttt > 1:2922917/1‑71 (MQ=255) ggCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGt < 1:1587023/71‑1 (MQ=255) ggCCATGATATTTTCCCACTCGGCATCTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGt < 1:4182492/71‑1 (MQ=255) ccATGATATTTTCCCACTCGGCATTTAATGATTCGTTaa < 1:2877652/39‑1 (MQ=255) tGATATTTTCCCACTCGGCATTTAATGATTCGTTAAc < 1:429423/37‑1 (MQ=255) atatTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATa < 1:1011454/71‑1 (MQ=255) atatTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATa < 1:2044840/71‑1 (MQ=255) tatTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCttt > 1:695874/1‑60 (MQ=255) tatTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACAt > 1:4232976/1‑69 (MQ=255) tatTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACAt > 1:2181137/1‑69 (MQ=255) tatTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATa < 1:4651388/70‑1 (MQ=255) tatTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGATTTACTGTACAAt > 1:1487662/1‑55 (MQ=255) tttCCCACTCGGCATCTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGt > 1:4837024/1‑60 (MQ=255) ttCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAg > 1:3553381/1‑71 (MQ=255) ttCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAg > 1:3509956/1‑71 (MQ=255) cACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATcc > 1:1823082/1‑49 (MQ=255) cACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATcc > 1:1607436/1‑49 (MQ=255) cACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCa > 1:2917339/1‑71 (MQ=255) cACTCGGCATCTAATGATTCGTTAACAAATGCGCtt < 1:2176027/36‑1 (MQ=255) cACTCGGCATCTAATGATTCGTTAACAAATGCGCtt < 1:3347848/36‑1 (MQ=255) aCTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCAt < 1:120042/71‑1 (MQ=255) cTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATaa > 1:2412884/1‑61 (MQ=255) cTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATaa > 1:2311554/1‑61 (MQ=255) cTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCAt < 1:2942335/70‑1 (MQ=255) cTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCAt < 1:1045728/70‑1 (MQ=255) tCGGCATCTAATGATTCGTTAACAAATGCGCTTTACTg > 1:4175602/1‑38 (MQ=255) cGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCAtt > 1:5100418/1‑69 (MQ=255) ggCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTaa > 1:4920299/1‑53 (MQ=255) ggCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTa < 1:3209316/69‑1 (MQ=255) ggCATCTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTa > 1:1452570/1‑69 (MQ=255) aTTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATgcg > 1:3431804/1‑70 (MQ=255) aTTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATgcg > 1:190838/1‑70 (MQ=255) tttAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCAt < 1:2920857/63‑1 (MQ=255) | GAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCG > W3110S.gb/2218858‑2218995 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |