Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	2,238,432	T→C	32.8%	L356L (TTA→CTA)	yeiT →	predicted oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,238,432	0	T	C	32.8%	64.3 / 53.4	58	L356L (TTA→CTA)	yeiT	predicted oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (24/15);  new base C (13/6);  total (37/21)
Fisher's exact test for biased strand distribution p-value = 7.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

ACGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCTTT  >  W3110S.gb/2238366‑2238494                                                                   |                                                               aCGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTa                                                              >  1:2064993/1‑69 (MQ=255)         ggcAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGc                                                    >  1:526465/1‑71 (MQ=255)              aTAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGt                                                  <  1:2958055/68‑1 (MQ=255)              aTAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGt                                                  <  1:2756362/68‑1 (MQ=255)              aTAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGc                                                    <  1:2024427/66‑1 (MQ=255)               tAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAaca                                              >  1:2540462/1‑71 (MQ=255)               tAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAaca                                              >  1:4059239/1‑71 (MQ=255)                aaaaTTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAacac                                             >  1:3902029/1‑71 (MQ=255)                   attattCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTaaaa                                              >  1:4341752/1‑65 (MQ=255)                   attattCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCAt                                          >  1:3198198/1‑71 (MQ=255)                       ttCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCaa                                       <  1:1915178/70‑1 (MQ=255)                       ttCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCaa                                       <  1:2554426/70‑1 (MQ=255)                       ttCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCaa                                       <  1:2175833/70‑1 (MQ=255)                       ttCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCaa                                       <  1:4946461/70‑1 (MQ=255)                       ttCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCaa                                       <  1:1520876/70‑1 (MQ=255)                            gCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAacac                                  >  1:3946936/1‑70 (MQ=255)                             ccGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaa                                <  1:2186634/71‑1 (MQ=255)                             ccGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACaa                                <  1:3757812/71‑1 (MQ=255)                             ccGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACaa                                <  1:3751611/71‑1 (MQ=255)                                tCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGtt                                                               <  1:992404/37‑1 (MQ=255)                                tCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGtt                                                               <  1:4701033/37‑1 (MQ=255)                                  ggTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaaaa                              >  1:4761170/1‑68 (MQ=255)                                  ggTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaaaa                              >  1:3698899/1‑68 (MQ=255)                                    tCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaa                                <  1:15340/64‑1 (MQ=255)                                    tCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaa                                <  1:3615097/64‑1 (MQ=255)                                        cATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgac                     >  1:1213285/1‑71 (MQ=255)                                         aTGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacc                    <  1:512319/71‑1 (MQ=255)                                          tGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCaa                                       <  1:2129479/51‑1 (MQ=255)                                          tGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacc                    >  1:2840664/1‑70 (MQ=255)                                            ccAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaa                                >  1:3695136/1‑56 (MQ=255)                                              aGACTAGATGCCTTTGCGTAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcga               >  1:875665/1‑71 (MQ=255)                                              aGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcga               >  1:474733/1‑71 (MQ=255)                                              aGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcga               >  1:4603075/1‑71 (MQ=255)                                                  tAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcgacccg           >  1:3595992/1‑71 (MQ=255)                                                    gATGCCTTTGCGGTGCTAGAGCCGCAGCGTAACACCATCAAAAcaca                                 >  1:1751139/1‑47 (MQ=39)                                                    gATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcgacccgc          >  1:2671369/1‑70 (MQ=255)                                                    gATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAAcaca                                 >  1:442798/1‑47 (MQ=255)                                                    gATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAAcaca                                 >  1:4187449/1‑47 (MQ=255)                                                    gATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTa                           >  1:1872444/1‑53 (MQ=255)                                                     aTGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaa                                <  1:4626494/47‑1 (MQ=255)                                                     aTGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgaccc                   <  1:4041644/60‑1 (MQ=255)                                                      tGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTAc                          <  1:387710/52‑1 (MQ=255)                                                       gCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaaaa                              >  1:2081545/1‑47 (MQ=255)                                                       gCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaaa                               >  1:2823976/1‑46 (MQ=255)                                                       gCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCaa        >  1:4709533/1‑69 (MQ=255)                                                       gCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCaa        >  1:1699240/1‑69 (MQ=255)                                                       gCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAg       >  1:612582/1‑70 (MQ=255)                                                          tttGCGGAGTTAGAGCCGCAGCGTAACACCATCaaaa                                     <  1:2732283/37‑1 (MQ=255)                                                            tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaaaa                              >  1:584410/1‑42 (MQ=255)                                                            tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaaaa                              >  1:66035/1‑42 (MQ=255)                                                            tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTAc                          >  1:1776195/1‑46 (MQ=255)                                                            tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTAc                          >  1:4838791/1‑46 (MQ=255)                                                            tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTAc                          >  1:3045361/1‑46 (MQ=255)                                                            tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTAc                          >  1:409482/1‑46 (MQ=255)                                                            tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgac                     >  1:3925040/1‑51 (MQ=255)                                                            tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAga                      >  1:856933/1‑50 (MQ=255)                                                            tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCttt  >  1:3250628/1‑70 (MQ=255)                                                            tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCttt  >  1:16653/1‑70 (MQ=255)                                                            tGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCttt  <  1:1486373/70‑1 (MQ=255)                                                            tGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCtt   <  1:1863101/69‑1 (MQ=255)                                                                   |                                                               ACGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCTTT  >  W3110S.gb/2238366‑2238494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑