| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,266,989 | T→C | 39.1% | intergenic (‑160/‑208) | fruB ← / → setB | fused fructose‑specific PTS enzyme IIA component and HPr component/lactose/glucose efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,266,989 | 0 | T | C | 39.1% | 44.4 / 81.6 | 69 | intergenic (‑160/‑208) | fruB/setB | fused fructose‑specific PTS enzyme IIA component and HPr component/lactose/glucose efflux system |
| Reads supporting (aligned to +/- strand): ref base T (19/23); new base C (16/11); total (35/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
AACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCAGCAATT > W3110S.gb/2266921‑2267056 | aaCGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGttt < 1:4564027/71‑1 (MQ=255)aaCGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGctt < 1:4076531/71‑1 (MQ=255) aCGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTg < 1:474141/71‑1 (MQ=255) aCGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTg < 1:1620250/71‑1 (MQ=255) aCGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTg < 1:1738853/71‑1 (MQ=255) aCGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTg < 1:4816101/71‑1 (MQ=255) aCGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTg < 1:1963402/71‑1 (MQ=255) tttCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGCTTGa < 1:1240589/69‑1 (MQ=255) tCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGCTTGAtgtg > 1:1392108/1‑71 (MQ=255) cAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGAtgtg < 1:3628984/70‑1 (MQ=255) cAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGAtgtg < 1:1339278/70‑1 (MQ=255) aGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGtt > 1:3309929/1‑61 (MQ=255) aGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGtt > 1:963445/1‑61 (MQ=255) aGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGtt > 1:4840473/1‑61 (MQ=255) gAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGCTTGATGtgc > 1:4465514/1‑68 (MQ=255) aaaGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGca > 1:4051939/1‑71 (MQ=255) aaGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGctt < 1:409243/59‑1 (MQ=255) aataCTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTg < 1:5139197/54‑1 (MQ=255) aataCTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAAtttt > 1:1006233/1‑71 (MQ=255) taCTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAAtttttt > 1:3126178/1‑71 (MQ=255) taCTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGCTTGATGTGCTGCACAAtttttt > 1:4494495/1‑71 (MQ=255) aCTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTg > 1:2725460/1‑71 (MQ=255) aCTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTg > 1:831222/1‑71 (MQ=255) ttCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGCTTGa > 1:326758/1‑50 (MQ=255) tGTTTCAGCACGCAAGTAAAGCCGTAAAGTTATGAGCAAAAGttt < 1:3088703/45‑1 (MQ=255) tttCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTg < 1:4265771/44‑1 (MQ=255) tttCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAtttt < 1:2141078/71‑1 (MQ=255) tttCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAtttt > 1:1687162/1‑71 (MQ=255) ttCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAttttt > 1:2579476/1‑71 (MQ=255) ttCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAttttt > 1:512098/1‑71 (MQ=255) aGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTg > 1:1331366/1‑40 (MQ=255) aGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGCTTGa > 1:273231/1‑41 (MQ=255) cGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGctt < 1:4208049/35‑1 (MQ=255) cGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAAtt > 1:3332700/1‑71 (MQ=255) gCAAGGAAAGCCGTAAAGTTATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTg < 1:3843446/71‑1 (MQ=255) cAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGa < 1:263630/35‑1 (MQ=255) aaGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCt > 1:4699648/1‑71 (MQ=255) aGGAAAGCCGTAAAGTTATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCt > 1:1680711/1‑70 (MQ=255) ggAAAGCCGTAAAGTTATGAGCAAAAGCTTGATGTGCTGCACaa > 1:4187286/1‑44 (MQ=255) aaGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATgg > 1:2357256/1‑71 (MQ=255) aaGCCGTAAAGTTATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATgg < 1:2769778/71‑1 (MQ=255) aGCCGTAAAGTTATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATgg > 1:448281/1‑70 (MQ=255) gCCGTAAAGTTATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGa < 1:52202/71‑1 (MQ=255) ccGTAAAGTTATGAGCAAAAGTTTGATGTGCTTCACAAtttt < 1:966764/42‑1 (MQ=255) gTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAt < 1:4743956/47‑1 (MQ=255) gTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAt < 1:890767/47‑1 (MQ=255) gTAAAGTTATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGaaaa < 1:191861/71‑1 (MQ=255) tAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCt < 1:2913733/53‑1 (MQ=255) tAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCt < 1:2443196/53‑1 (MQ=255) tAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAAt > 1:3898120/1‑71 (MQ=255) tAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAAt > 1:551657/1‑71 (MQ=255) tAAAGTCATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAt < 1:1573666/46‑1 (MQ=255) aaGTTATGAGCAAAAGTTTGATGTGCTGCACAAttttt > 1:2266735/1‑38 (MQ=255) aaGTTATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATcc > 1:4759421/1‑71 (MQ=255) ttATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGtt < 1:2811805/71‑1 (MQ=255) ttATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGtt < 1:2235904/71‑1 (MQ=255) ttATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGtt < 1:4642320/71‑1 (MQ=255) ttATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGaa < 1:684177/63‑1 (MQ=255) ttATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGaa < 1:99503/63‑1 (MQ=255) ttATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGtt < 1:491420/71‑1 (MQ=255) ttATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTCATGGGaa < 1:2129279/63‑1 (MQ=255) tATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTa > 1:2867051/1‑71 (MQ=255) aTGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGc > 1:1863531/1‑52 (MQ=255) aTGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGc > 1:3295721/1‑52 (MQ=255) aTGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGc > 1:3146152/1‑52 (MQ=255) tGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTAt > 1:4847259/1‑70 (MQ=255) aGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTaa > 1:4789576/1‑45 (MQ=255) aGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTaa > 1:934840/1‑45 (MQ=255) aGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTaa > 1:4073569/1‑45 (MQ=255) cAACAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCAGc < 1:4679823/70‑1 (MQ=255) cAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCt < 1:1970119/41‑1 (MQ=255) cAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCt < 1:1924943/41‑1 (MQ=255) cAAAAGCTTGATGTGCTGGACAATTTTTTGCCATTTTTc < 1:1027292/39‑1 (MQ=37) aaaaGTTTTATGTGCTGCACAATTTTTTGCCATTTTTCCt < 1:1289742/40‑1 (MQ=255) aaaGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTaa > 1:4482010/1‑41 (MQ=255) aaaGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTaa > 1:913891/1‑41 (MQ=255) aaGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCAGCAatt > 1:4626194/1‑71 (MQ=255) | AACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCAGCAATT > W3110S.gb/2266921‑2267056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |