| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,331,670 | C→T | 10.8% | Q298* (CAA→TAA) | atoB → | acetyl‑CoA acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,331,670 | 0 | C | T | 10.8% | 136.4 / 6.1 | 46 | Q298* (CAA→TAA) | atoB | acetyl‑CoA acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (21/20); new base T (2/3); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGC > W3110S.gb/2331604‑2331740 | aTTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCaaaa < 1:827875/71‑1 (MQ=255)aTTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCaaa > 1:4020536/1‑70 (MQ=255) tAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAg > 1:522254/1‑71 (MQ=255) aaaaGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGc > 1:4042896/1‑71 (MQ=255) aaaGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGc < 1:3739160/70‑1 (MQ=255) ttATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTa > 1:4896537/1‑70 (MQ=255) ttATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTAc < 1:207413/71‑1 (MQ=255) tATGCCAGCGGTGGCGTGCCCCCCGCATT‑ATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACa < 1:4464146/70‑1 (MQ=255) gCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCg < 1:4474953/71‑1 (MQ=255) gTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCa < 1:4365173/71‑1 (MQ=255) ccccccGCATTGATGGGTATGGGGCCAGTACCTGCCACGTAAAAAGCGTTACaa > 1:3055166/1‑54 (MQ=255) cccGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCa < 1:621371/65‑1 (MQ=255) ccGCATTGATGGGTATGGGGCCAGTACCTGCCACGTAAAAAGCGtt < 1:2765323/46‑1 (MQ=255) cGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCg > 1:1308469/1‑70 (MQ=255) gCATTGATGGGTATGGGGCCAGTACCTGCCACGTAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGa < 1:3116342/71‑1 (MQ=255) gCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGa > 1:86422/1‑71 (MQ=255) gCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGa < 1:1646750/71‑1 (MQ=255) cATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGc < 1:1681882/60‑1 (MQ=255) ttGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGc < 1:3592267/38‑1 (MQ=255) ttGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGc < 1:1049759/38‑1 (MQ=255) gATGGGTATGGGGCCAGTACCTGCCACGTAAAAAGCGTTa > 1:3639632/1‑40 (MQ=255) gATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGGGGATAtt > 1:1196177/1‑70 (MQ=255) gATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATAtt > 1:4584138/1‑70 (MQ=255) gATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATAtt > 1:1745821/1‑70 (MQ=255) tGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGAt > 1:4510426/1‑71 (MQ=255) gTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGAtctc < 1:2509293/71‑1 (MQ=255) gTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGAtctc < 1:249195/71‑1 (MQ=255) tGGGGCCAGTACCTGCCACGCAAAAAGCGTTACaa < 1:3668247/35‑1 (MQ=255) tGGGGCCAGTACCTGCCACGCAAAAAGCGTTACaa < 1:5015617/35‑1 (MQ=255) tGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCAtt > 1:2762303/1‑71 (MQ=255) gTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGAtct < 1:4035522/59‑1 (MQ=255) gTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTaa > 1:712674/1‑71 (MQ=255) gTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTa > 1:127476/1‑70 (MQ=255) tACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTg < 1:836280/63‑1 (MQ=255) tACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAAt > 1:1454443/1‑71 (MQ=255) aCCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAAt > 1:4863067/1‑70 (MQ=255) ccTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGAt > 1:3782140/1‑54 (MQ=255) ccTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGAt > 1:4183769/1‑54 (MQ=255) ccTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGAt > 1:3995058/1‑54 (MQ=255) cTGCCACGTAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGaa < 1:5057635/71‑1 (MQ=255) ccACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCa < 1:4801056/71‑1 (MQ=255) ccACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCa > 1:1142389/1‑71 (MQ=255) ccACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAAGGAAGCa < 1:4926/71‑1 (MQ=255) cACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCAt > 1:3005589/1‑71 (MQ=255) cGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCAttt < 1:1821021/71‑1 (MQ=255) gCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTtg > 1:3067086/1‑71 (MQ=255) cAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTtgc > 1:4428151/1‑71 (MQ=255) | ATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGC > W3110S.gb/2331604‑2331740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |