| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,358,400 | G→A | 39.7% | A362T (GCT→ACT) | glpA → | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,358,400 | 0 | G | A | 39.7% | 54.5 / 75.1 | 68 | A362T (GCT→ACT) | glpA | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (14/27); new base A (10/17); total (24/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACT > W3110S.gb/2358333‑2358459 | tGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCt > 1:3262406/1‑70 (MQ=255) aaCGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGa < 1:939553/70‑1 (MQ=255) aaCGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGa < 1:4056853/70‑1 (MQ=255) aaCGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGa < 1:1573309/70‑1 (MQ=255) aCGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAAt < 1:1646965/71‑1 (MQ=255) cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATg > 1:2587166/1‑71 (MQ=255) cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATg > 1:1547508/1‑71 (MQ=255) cgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATggg > 1:3099153/1‑71 (MQ=255) cgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATggg > 1:3261395/1‑71 (MQ=255) aTGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCt < 1:3030319/71‑1 (MQ=255) aTGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCt < 1:1645779/71‑1 (MQ=255) tGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTa < 1:1893016/71‑1 (MQ=255) tGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTa < 1:2383475/71‑1 (MQ=255) gTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTAcc < 1:3752230/71‑1 (MQ=255) cTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGa > 1:1628095/1‑71 (MQ=255) cTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGa > 1:4229421/1‑71 (MQ=255) cTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGa > 1:3627640/1‑71 (MQ=255) tGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGAc > 1:922455/1‑71 (MQ=255) cGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCgg < 1:684620/71‑1 (MQ=255) cGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCgg > 1:1259274/1‑71 (MQ=255) cGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCgg > 1:1265390/1‑71 (MQ=255) ggATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGt < 1:4398377/71‑1 (MQ=255) tttATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATg < 1:2459821/71‑1 (MQ=255) tttATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATg < 1:3694580/71‑1 (MQ=255) tttATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATg < 1:640508/71‑1 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATggg > 1:3020002/1‑51 (MQ=255) accatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCa < 1:825175/70‑1 (MQ=255) accatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCa > 1:2931035/1‑70 (MQ=255) atcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACt < 1:3259035/71‑1 (MQ=255) atcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACt < 1:2930791/71‑1 (MQ=255) tcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTg > 1:3779243/1‑71 (MQ=255) ccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGc < 1:1320159/71‑1 (MQ=255) tGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCaaa < 1:1055565/61‑1 (MQ=255) tGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCaaa < 1:1043536/61‑1 (MQ=255) tGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCaaa < 1:2682803/61‑1 (MQ=255) aaCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:1319887/1‑71 (MQ=255) aaCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc < 1:3739276/71‑1 (MQ=255) aaCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgagc > 1:944984/1‑71 (MQ=255) aaCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGt > 1:3496296/1‑46 (MQ=255) aCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGcc < 1:3682993/71‑1 (MQ=255) cTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAaca > 1:222086/1‑64 (MQ=255) atgaCCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTg < 1:4918785/71‑1 (MQ=255) atgaCCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTg < 1:4232842/71‑1 (MQ=255) tgaCCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATg < 1:5100001/43‑1 (MQ=255) aCCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTAc < 1:368778/71‑1 (MQ=255) aCCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTAc < 1:2003423/71‑1 (MQ=255) cTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGc < 1:721118/52‑1 (MQ=255) cTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGc < 1:2130123/52‑1 (MQ=255) cTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGc < 1:4733777/52‑1 (MQ=255) cTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGc < 1:4054979/52‑1 (MQ=255) cTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGc < 1:4269912/52‑1 (MQ=255) tATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCaa < 1:947283/44‑1 (MQ=255) tATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCaa < 1:3266548/44‑1 (MQ=255) aTCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGa > 1:1571072/1‑69 (MQ=255) aTCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACt > 1:2043388/1‑71 (MQ=255) tCGGCTGATGGCTGAATGGGCTACCGACGCTGTATGCCGCAAACTGGGc < 1:110747/49‑1 (MQ=255) tCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTg > 1:209080/1‑46 (MQ=255) tCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGc < 1:2937066/49‑1 (MQ=255) tCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTAc < 1:3634415/66‑1 (MQ=255) tCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGAc < 1:1460409/69‑1 (MQ=255) tCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACt < 1:4279068/70‑1 (MQ=255) tCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACt < 1:866307/70‑1 (MQ=255) tCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCa < 1:2517172/41‑1 (MQ=255) tCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCa < 1:2874291/41‑1 (MQ=255) tCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAAc > 1:1622434/1‑44 (MQ=255) cGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTggg > 1:809028/1‑47 (MQ=255) cGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGc < 1:3574304/48‑1 (MQ=255) ggCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCaaa > 1:2754237/1‑41 (MQ=255) | TGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACT > W3110S.gb/2358333‑2358459 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |