| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 108,684 | G→A | 9.1% | A136T (GCG→ACG) | secA → | preprotein translocase subunit, ATPase that targets protein precursors to the SecYE core translocon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 108,684 | 0 | G | A | 9.1% | 171.4 / 6.2 | 55 | A136T (GCG→ACG) | secA | preprotein translocase subunit, ATPase that targets protein precursors to the SecYE core translocon |
| Reads supporting (aligned to +/- strand): ref base G (20/30); new base A (2/3); total (22/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
ACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTCGGTATCAACCTGCC > W3110S.gb/108615‑108754 | aCGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGgc > 1:3976339/1‑71 (MQ=255) cGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGgcg > 1:2740806/1‑71 (MQ=255) gCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGgcgc < 1:3631782/71‑1 (MQ=255) gCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGgcgc < 1:501953/71‑1 (MQ=255) gCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGgcgc < 1:4397629/71‑1 (MQ=255) gCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGgcg > 1:4598612/1‑70 (MQ=255) ctgcctgcTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGgcgc > 1:4380237/1‑70 (MQ=255) ctgcctgcTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGACGCa < 1:746333/71‑1 (MQ=255) ctgcctgcTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGACGCa < 1:628845/71‑1 (MQ=255) ctgcctgcTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGACGCa < 1:1481159/71‑1 (MQ=255) cctgcTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACg > 1:4302725/1‑71 (MQ=255) ctgcTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGt > 1:3353293/1‑71 (MQ=255) ctgcTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGt < 1:1154035/71‑1 (MQ=255) cTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGAc > 1:3960825/1‑71 (MQ=255) ccTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGcc < 1:4746769/70‑1 (MQ=255) gAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGaa > 1:4190489/1‑70 (MQ=255) cTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCg < 1:2829358/71‑1 (MQ=255) cTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCg > 1:3076552/1‑71 (MQ=255) aaCCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGACGCAACGTGACGCCGAAAACAACCGTc > 1:3595296/1‑71 (MQ=255) aCCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGACGCAACGTGACGCCGAAAACAACCGTc > 1:1071098/1‑70 (MQ=255) gTAAAGGCGTGCACGTAGTTACCGTCCACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCctct > 1:2784699/1‑70 (MQ=255) aaaGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGt > 1:1599535/1‑70 (MQ=255) aGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTg < 1:4493451/71‑1 (MQ=255) aGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTg < 1:4278638/71‑1 (MQ=255) aGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTg < 1:2403843/71‑1 (MQ=255) aGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTg < 1:1907617/71‑1 (MQ=255) aGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTg < 1:4120265/71‑1 (MQ=255) aGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTg < 1:4091940/71‑1 (MQ=255) ggCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGa < 1:4205949/71‑1 (MQ=255) cACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTcc < 1:1960262/70‑1 (MQ=255) cACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCt > 1:3041272/1‑71 (MQ=255) tAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGc < 1:3835706/71‑1 (MQ=255) gTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCt < 1:4068430/71‑1 (MQ=255) gTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCt < 1:3616245/71‑1 (MQ=255) ttACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCg < 1:4531314/46‑1 (MQ=255) ttACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCg < 1:1475453/46‑1 (MQ=255) ttACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCg < 1:2358939/46‑1 (MQ=255) ttACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGc < 1:3311754/51‑1 (MQ=255) ttACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAAt < 1:766028/60‑1 (MQ=255) ttACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAAt < 1:5095571/60‑1 (MQ=255) ttACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCt > 1:1036712/1‑70 (MQ=255) ttACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCt > 1:1754682/1‑70 (MQ=255) tACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGa > 1:2071655/1‑71 (MQ=255) tACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGa > 1:738182/1‑71 (MQ=255) aaCCGGCAACGACTACCTGGCGCCACGTGACGCCGAAAACAACCg < 1:1864463/44‑1 (MQ=255) aCCGTCAACGAGTACCTGGCGCAACGTGACGCCGAAAACAACCATCCGCTGTTTGaa > 1:3591925/1‑57 (MQ=255) aCCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGaa > 1:2160982/1‑57 (MQ=255) aCCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGaa > 1:1431891/1‑57 (MQ=255) cGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTg < 1:1126285/53‑1 (MQ=255) cGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTc < 1:3063738/58‑1 (MQ=255) tCAACGACTACCTGGCGCAACGTGACGCCGAAaaca < 1:984590/36‑1 (MQ=255) tCAACGACTACCTGGCGCAACGTGACGCCGAAaaca < 1:3239/36‑1 (MQ=255) tCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTc < 1:1828873/71‑1 (MQ=255) aaCGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGttt > 1:3213563/1‑48 (MQ=255) aaCGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGaa > 1:3926787/1‑51 (MQ=255) aaCGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGaa > 1:4066813/1‑51 (MQ=255) aaCGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTCg > 1:334394/1‑70 (MQ=255) aCGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTc > 1:3474379/1‑53 (MQ=255) ccTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTCGGTATCaa < 1:3285073/69‑1 (MQ=255) ccTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTCGGTATCaa < 1:12658/69‑1 (MQ=255) cTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTg < 1:334643/49‑1 (MQ=255) tGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAAt < 1:311438/42‑1 (MQ=255) ggCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGaa > 1:4585652/1‑40 (MQ=255) ggCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGaa > 1:492683/1‑40 (MQ=255) ggCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGaa > 1:1545074/1‑40 (MQ=255) ggCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTCGGTATCAACCTGc > 1:4655788/1‑71 (MQ=255) gcgcAACGTGACGCCGAAAACAACCGTCCGCTGTTTg < 1:1041785/37‑1 (MQ=255) gcgcAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTCGGTATCAACCTGcc > 1:1828697/1‑71 (MQ=255) | ACGCTGCCTGCTTACCTGAACGCACTAACCGGTAAAGGCGTGCACGTAGTTACCGTCAACGACTACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTCGGTATCAACCTGCC > W3110S.gb/108615‑108754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |