| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,387,067 | G→A | 16.0% | G264S (GGC→AGC) | elaC → | binuclear zinc phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,387,067 | 0 | G | A | 16.0% | 127.6 / 15.5 | 50 | G264S (GGC→AGC) | elaC | binuclear zinc phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (18/24); new base A (4/4); total (22/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01 | |||||||||||
GCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATG > W3110S.gb/2386998‑2387137 | gccaaagccaaTAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCgg > 1:352642/1‑71 (MQ=255) agccaaTAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGc < 1:1702792/71‑1 (MQ=255) agccaaTAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGc < 1:4761027/71‑1 (MQ=255) ccaaTAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCt < 1:2229575/70‑1 (MQ=255) caaTAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTa < 1:4182293/70‑1 (MQ=255) gTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCaa > 1:741069/1‑61 (MQ=255) gTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCac > 1:1228334/1‑69 (MQ=255) gTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCAtt < 1:1727264/71‑1 (MQ=255) gTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCAt > 1:728509/1‑70 (MQ=255) gTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCAt < 1:866713/70‑1 (MQ=255) gTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCAt > 1:3075594/1‑70 (MQ=255) tCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCAtt < 1:1209126/70‑1 (MQ=255) gCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTa < 1:688787/59‑1 (MQ=255) gCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCAGCAAGCTAATCATTACCCAc < 1:924732/71‑1 (MQ=255) aTAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTc < 1:4704540/71‑1 (MQ=255) aGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAg > 1:4589815/1‑71 (MQ=255) gCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAg > 1:3697349/1‑70 (MQ=255) gCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAg < 1:1775825/70‑1 (MQ=255) gCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAg < 1:1650144/70‑1 (MQ=255) gCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGc < 1:2936122/71‑1 (MQ=255) gCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGc < 1:982472/71‑1 (MQ=255) gCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGc < 1:4164943/71‑1 (MQ=255) ctctACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCAGCAAGCTAATCATTACCCACGt < 1:1999191/66‑1 (MQ=255) ctctACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCAGCAAGCTAATCATTACCAACGt < 1:631919/66‑1 (MQ=255) tACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGt < 1:445600/63‑1 (MQ=255) tACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTcgc > 1:1492057/1‑71 (MQ=255) tACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTcg > 1:2843379/1‑70 (MQ=255) cacGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCAGCAAGCTAATCa > 1:3211080/1‑51 (MQ=255) cacGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCAGCAAGCTAATCa > 1:1675801/1‑51 (MQ=255) cTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGAc < 1:87354/71‑1 (MQ=255) tGCGACACTAGCCCGTGAGGCTGGAGTCAGCAAGCTAATCATTACCCACGTCAGCTCGCGCTAtgatga > 1:615180/1‑69 (MQ=255) gCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCa > 1:3646457/1‑52 (MQ=255) gCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCa > 1:3320310/1‑52 (MQ=255) gCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACa > 1:3269496/1‑70 (MQ=255) tAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCa > 1:4692463/1‑45 (MQ=255) tAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCATCTCGCGCTATGATGACAAAGGTTGt > 1:1999803/1‑71 (MQ=255) tAGCCCGTGAGGCTGCAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGt > 1:4984069/1‑71 (MQ=255) aGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGt > 1:985789/1‑70 (MQ=255) gCCCGTGAGGCTGGAGTCAGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGt < 1:3594714/69‑1 (MQ=255) gCCCCTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTTTGATGACAAAGGTTGt < 1:905805/69‑1 (MQ=255) cGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCa > 1:3050123/1‑71 (MQ=255) cGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCa > 1:533878/1‑71 (MQ=255) aGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTg < 1:3944056/71‑1 (MQ=255) aGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTg < 1:793739/71‑1 (MQ=255) ggCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGt < 1:4038960/71‑1 (MQ=255) tGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTAGCGCTATGATGACAAAGGTTGTCAGCACCTGTTAc > 1:1515058/1‑71 (MQ=255) ggAGTCAGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACg > 1:768656/1‑71 (MQ=255) aGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTg < 1:4845124/71‑1 (MQ=255) tCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTc < 1:1409496/54‑1 (MQ=255) tCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTc < 1:2399709/54‑1 (MQ=255) tCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGAAAAAGGTTGTc < 1:2853190/54‑1 (MQ=255) ggCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAg > 1:3496321/1‑54 (MQ=255) ggCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATg < 1:4620155/71‑1 (MQ=255) ggCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATg > 1:4165802/1‑71 (MQ=255) ggCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATg < 1:140042/71‑1 (MQ=255) ggCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATg < 1:3148969/71‑1 (MQ=255) | GCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATG > W3110S.gb/2386998‑2387137 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |