| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,389,320 | T→C | 6.7% | N358S (AAC→AGC) | yfbK ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,389,320 | 0 | T | C | 6.7% | 253.8 / 8.6 | 90 | N358S (AAC→AGC) | yfbK | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (47/37); new base C (2/4); total (49/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01 | |||||||||||
AGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGAC > W3110S.gb/2389253‑2389384 | agagagGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGt < 1:394037/71‑1 (MQ=255)agagagGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGt < 1:3757946/71‑1 (MQ=255) gagGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAAt < 1:3571586/71‑1 (MQ=255) gagGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAAt < 1:392039/71‑1 (MQ=255) agGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAAtt > 1:2884769/1‑71 (MQ=255) ggTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGc < 1:1261602/71‑1 (MQ=255) tATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGc < 1:1417757/69‑1 (MQ=255) aTCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTAt > 1:1488686/1‑71 (MQ=255) tCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGtt > 1:4071957/1‑59 (MQ=255) tCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGtt > 1:5098175/1‑59 (MQ=255) tCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGt < 1:4677510/61‑1 (MQ=255) tCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGt < 1:1392979/61‑1 (MQ=255) tCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGt < 1:3599607/61‑1 (MQ=255) cAATGTAGCTGTAGTTGCCGTTAGCAACATCGGCAATTCGCACCATCATTGCCTCGtt > 1:1506158/1‑58 (MQ=255) aaTGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTcc > 1:2292503/1‑71 (MQ=255) aaTGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTcc > 1:725866/1‑71 (MQ=255) aaTGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTcc > 1:4068158/1‑71 (MQ=255) aaTGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTcc > 1:4149921/1‑71 (MQ=255) gTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCa > 1:3997411/1‑71 (MQ=255) cTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGc > 1:1138892/1‑70 (MQ=255) gTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCa > 1:535573/1‑62 (MQ=255) gTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCa > 1:568262/1‑62 (MQ=255) gTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCa > 1:3484983/1‑62 (MQ=255) ttGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGc < 1:3021804/52‑1 (MQ=255) ttGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGc < 1:290790/52‑1 (MQ=255) ttGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGc < 1:283635/52‑1 (MQ=255) tGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTc > 1:2587517/1‑71 (MQ=255) gCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCt > 1:1590728/1‑51 (MQ=255) gCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCa > 1:4856618/1‑59 (MQ=255) gCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTc < 1:2698156/70‑1 (MQ=255) gCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTc < 1:4038803/70‑1 (MQ=255) gCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTc > 1:2910379/1‑70 (MQ=255) gCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTc > 1:1659910/1‑70 (MQ=255) gCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTc > 1:3323940/1‑70 (MQ=255) gCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTc < 1:2235075/70‑1 (MQ=255) ccGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTaa > 1:4468035/1‑45 (MQ=255) ccGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGa < 1:2041755/71‑1 (MQ=255) cGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTaa > 1:4725047/1‑44 (MQ=255) cGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTaa > 1:3729442/1‑44 (MQ=255) gTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACa < 1:1567436/71‑1 (MQ=255) gTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACa < 1:2982334/71‑1 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTAtt < 1:4231989/50‑1 (MQ=255) ttACCAACATCGGCAATTAGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGa > 1:2699113/1‑68 (MQ=255) aCCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACaga < 1:1699475/70‑1 (MQ=255) ccAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACagag < 1:2000974/70‑1 (MQ=255) aaCATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTAt > 1:1728975/1‑44 (MQ=255) aaCATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTc > 1:2667202/1‑46 (MQ=255) aCATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAac < 1:162241/71‑1 (MQ=255) cATCGGCAATTCGCACCATCATTGCCTCGTTGTaa > 1:4075411/1‑35 (MQ=255) cATCGGCAATTCGCACCATCATTGCCTCGTTGTaa > 1:5045662/1‑35 (MQ=255) cATCGGCAATTCGCACCATCATTGCCTCGTTGTaa > 1:15621/1‑35 (MQ=255) cATCGGCAATTCGCACCATCATTGCCTCGTTGTaa > 1:3919014/1‑35 (MQ=255) cATCGGCAATTCGCACCATCATTGCCTCGTTGTaa > 1:2149343/1‑35 (MQ=255) cATCGGCAATTCGCACCATCATTGCCTCGTTGTaa > 1:1672657/1‑35 (MQ=255) tCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAacac < 1:2457330/70‑1 (MQ=255) cGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAacac < 1:475484/69‑1 (MQ=255) cGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACAcc > 1:787069/1‑70 (MQ=255) cGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACAcc > 1:160417/1‑70 (MQ=255) gCAATTCGCACCATCATTTCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACaga < 1:4595619/60‑1 (MQ=255) gCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACaga < 1:4334477/60‑1 (MQ=255) cAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGAc < 1:887881/71‑1 (MQ=255) aaTTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGt > 1:4874988/1‑60 (MQ=255) aaTTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGt > 1:1719379/1‑60 (MQ=255) tCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAac < 1:4474079/60‑1 (MQ=255) tCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTccc > 1:4929173/1‑71 (MQ=255) tCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTccc > 1:4858317/1‑71 (MQ=255) cGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCa > 1:3944781/1‑42 (MQ=255) cGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCa > 1:3918730/1‑42 (MQ=255) cGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTccc < 1:4365671/70‑1 (MQ=255) cGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCg > 1:1350508/1‑71 (MQ=255) cGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCg > 1:874728/1‑71 (MQ=255) cACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGt < 1:1533474/54‑1 (MQ=255) cACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGt < 1:647453/54‑1 (MQ=255) cACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGt < 1:705581/54‑1 (MQ=255) ccATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCa < 1:1890721/59‑1 (MQ=255) ccATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGt > 1:631583/1‑71 (MQ=255) catcatTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt > 1:958716/1‑71 (MQ=255) catcatTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt < 1:1403312/71‑1 (MQ=255) catcatTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt > 1:1337518/1‑71 (MQ=255) catcatTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGt > 1:5055218/1‑70 (MQ=255) tcatTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGt < 1:285921/49‑1 (MQ=255) tcatTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCa < 1:3908025/56‑1 (MQ=255) tcatTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtttt < 1:315595/71‑1 (MQ=255) atTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGa > 1:1606889/1‑56 (MQ=255) atTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGa > 1:788244/1‑56 (MQ=255) atTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtttttt < 1:2228912/71‑1 (MQ=255) tGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGa > 1:3434786/1‑71 (MQ=255) gCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGAc > 1:2865149/1‑71 (MQ=255) ccTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGa > 1:3971507/1‑37 (MQ=255) ccTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGa > 1:92600/1‑37 (MQ=255) ccTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGAc < 1:1249298/70‑1 (MQ=255) tCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAacac < 1:4331410/46‑1 (MQ=255) cGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCa < 1:1550070/47‑1 (MQ=255) | AGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGAC > W3110S.gb/2389253‑2389384 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |