| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,400,279 | C→T | 27.0% | P96P (CCG→CCA) | nuoJ ← | NADH:ubiquinone oxidoreductase, membrane subunit J |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,400,279 | 0 | C | T | 27.0% | 109.7 / 42.2 | 63 | P96P (CCG→CCA) | nuoJ | NADH:ubiquinone oxidoreductase, membrane subunit J |
| Reads supporting (aligned to +/- strand): ref base C (23/23); new base T (11/6); total (34/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01 | |||||||||||
ACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGT > W3110S.gb/2400213‑2400343 | aCCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGAc > 1:4826301/1‑71 (MQ=255) ccTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCa < 1:1152218/71‑1 (MQ=255) ccTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCa < 1:3636098/71‑1 (MQ=255) ccTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCa < 1:3997583/71‑1 (MQ=255) cTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCaa < 1:672839/71‑1 (MQ=255) aTCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCTGGACCAATc > 1:3683209/1‑70 (MQ=255) aTCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAAt < 1:2781224/69‑1 (MQ=255) aTCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATc < 1:1039918/70‑1 (MQ=255) aTCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATc < 1:2343789/70‑1 (MQ=255) aTCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATc < 1:4162157/70‑1 (MQ=255) tCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCTGGACCAATcc > 1:3534861/1‑70 (MQ=255) tCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCTGGACCAATCca > 1:3151441/1‑71 (MQ=255) tCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCTGGACCAATCca > 1:5110454/1‑71 (MQ=255) tCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGAc > 1:3200582/1‑64 (MQ=255) tCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGAc > 1:1512623/1‑64 (MQ=255) tCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCca > 1:3709167/1‑71 (MQ=255) gTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCTGGACCAATCcac < 1:1902191/70‑1 (MQ=255) gTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCTGGACCAATCcac < 1:1929814/70‑1 (MQ=255) gTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCTGGACCAATCcac < 1:3948522/70‑1 (MQ=255) gTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCTGGACCAATCcac < 1:1678920/70‑1 (MQ=255) gTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCTGGACCAATCcac < 1:476226/70‑1 (MQ=255) gTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCcac < 1:769969/70‑1 (MQ=255) gTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCcac < 1:2692168/70‑1 (MQ=255) gTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCcac < 1:1983621/70‑1 (MQ=255) cGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGt > 1:950033/1‑71 (MQ=255) cGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGt > 1:2983915/1‑71 (MQ=255) cGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGt > 1:3022549/1‑71 (MQ=255) cGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGt > 1:207758/1‑71 (MQ=255) gAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGtt > 1:2720145/1‑71 (MQ=255) gAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGtt > 1:1025256/1‑71 (MQ=255) gCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGcc < 1:3545286/70‑1 (MQ=255) cGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCTGGACCAATCCACACCTGCGGTTTCAGCCAc > 1:4754455/1‑71 (MQ=255) cGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCAc > 1:4538728/1‑71 (MQ=255) aCAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCg < 1:880693/71‑1 (MQ=255) cAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCacac > 1:1177059/1‑49 (MQ=255) cAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCacac > 1:1873244/1‑49 (MQ=255) aTCACCACCAGCATGATGGCCGACAAAATTGCTGGACCAATCCacac > 1:4563786/1‑47 (MQ=255) aTCACCACCAGCATGATGGCCGACAAAATTGCTGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTc > 1:1838231/1‑71 (MQ=255) aTCACCACCAGCATGATGGCCGACAAAATTGCTGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTc > 1:3854056/1‑71 (MQ=255) tCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCca > 1:4631233/1‑43 (MQ=255) tCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCca > 1:2224388/1‑43 (MQ=255) tCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCca > 1:4539215/1‑43 (MQ=255) accaccAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCt < 1:1690369/70‑1 (MQ=255) accaccAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTg > 1:973043/1‑71 (MQ=255) ccaGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTg > 1:4948741/1‑67 (MQ=255) ccaGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTc > 1:247453/1‑70 (MQ=255) ccaGCATGATGGCCGACAAAATTGACGGACCAATCAACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTc > 1:2343854/1‑70 (MQ=255) aGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGAt < 1:1350859/71‑1 (MQ=255) gCATGATGGCCGACAAAATTGCCGGACCAATCcac > 1:2334242/1‑35 (MQ=255) atgatgGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCa < 1:1294121/50‑1 (MQ=255) atgatgGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCa < 1:4294712/50‑1 (MQ=255) atgatgGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTc > 1:4544034/1‑71 (MQ=255) atgGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTg > 1:315915/1‑70 (MQ=255) atgGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTg < 1:656739/70‑1 (MQ=255) gCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAAcc < 1:4620569/71‑1 (MQ=255) gCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAAcc < 1:4523211/71‑1 (MQ=255) gACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTg < 1:2339302/53‑1 (MQ=255) gACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGcc < 1:647116/71‑1 (MQ=255) cAAAATTGCTGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGcc > 1:554708/1‑69 (MQ=255) aaTTGCTGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGt > 1:1817824/1‑71 (MQ=255) aaTTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGcc < 1:641104/66‑1 (MQ=255) aTTGCTGTACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTcc < 1:3550855/45‑1 (MQ=38) aTTGCTGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGa > 1:444506/1‑52 (MQ=255) | ACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGT > W3110S.gb/2400213‑2400343 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |