| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,462,102 | A→G | 10.8% | F53L (TTC→CTC) | sixA ← | phosphohistidine phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,462,102 | 0 | A | G | 10.8% | 166.4 / 12.8 | 65 | F53L (TTC→CTC) | sixA | phosphohistidine phosphatase |
| Reads supporting (aligned to +/- strand): ref base A (31/27); new base G (2/5); total (33/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-01 | |||||||||||
AACTTCTGCACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCG > W3110S.gb/2462034‑2462172 | aaCTTCTGCACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAGCg < 1:1540475/71‑1 (MQ=255) cTGCACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGcg > 1:3065751/1‑69 (MQ=255) cTGCACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGc < 1:2207368/69‑1 (MQ=255) cTGCACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCt > 1:2009435/1‑70 (MQ=255) tGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAg > 1:29199/1‑70 (MQ=255) gAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGaa > 1:112186/1‑70 (MQ=255) gAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGaa > 1:4882251/1‑70 (MQ=255) aGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAAcgc < 1:2248573/71‑1 (MQ=255) aGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACTCGTTc < 1:1291985/71‑1 (MQ=255) aGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTc < 1:3211192/71‑1 (MQ=255) aCAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAGCGGGCTCACCAGAACGCGTTCGATTTCCa > 1:3875117/1‑71 (MQ=255) aCAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTc < 1:998194/63‑1 (MQ=255) aCAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTc < 1:2191408/63‑1 (MQ=255) aCAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTc < 1:4893146/63‑1 (MQ=255) cAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTc > 1:3592982/1‑62 (MQ=255) cAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTc > 1:3184606/1‑62 (MQ=255) cAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTc > 1:4778209/1‑62 (MQ=255) aaTCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAGCGGGCTCACCAGAACGCGTTCGATTTCCACt < 1:1502586/71‑1 (MQ=255) aaTCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAGCGGGCTCACCAGAACGCGTTCGATTTCCACt < 1:4908335/71‑1 (MQ=255) aaTCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAGCGGGCTCACCAGAACGCGTTCGATTTCCACt < 1:2764399/71‑1 (MQ=255) aaTCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCa > 1:3189249/1‑48 (MQ=255) aaTCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCa > 1:5123284/1‑48 (MQ=255) ccccTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACtttt > 1:4067070/1‑71 (MQ=255) cTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGa > 1:4302797/1‑47 (MQ=255) ttcttcCAGTGTTTGCTCGGCTCTCAGGAACGGGCTCACCAGAACGCGTTCg < 1:1198735/52‑1 (MQ=255) ttcttcCAGTGTTTGCTCGGCTCGCAGGAACGGGCTcagcag < 1:2971471/42‑1 (MQ=255) ttcttcCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAg < 1:1297332/42‑1 (MQ=255) cttcCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGaa > 1:435764/1‑42 (MQ=255) cttcCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGaa > 1:1969354/1‑42 (MQ=255) cttcCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTc < 1:3280542/49‑1 (MQ=255) cttcCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGAc > 1:2842153/1‑66 (MQ=255) cttcCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCttt < 1:1818203/70‑1 (MQ=255) ccAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGc > 1:4277926/1‑71 (MQ=255) ccAGTGATTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGc > 1:658565/1‑71 (MQ=255) gtTTGCTCGGCTCGCAGGAGCGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGtt > 1:1687200/1‑71 (MQ=255) tttGCTCGGCTCGCAGGAACGGGCTCACCAGAACTCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGt < 1:294322/69‑1 (MQ=255) tttGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTc < 1:5077910/40‑1 (MQ=255) tttGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTc < 1:150462/40‑1 (MQ=255) tttGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGt < 1:1714229/69‑1 (MQ=255) tGCTCGGCTCGCAGGAACGGGCTCCCCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGc > 1:2574617/1‑71 (MQ=255) tGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACttttt > 1:1044952/1‑52 (MQ=255) tGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACttttt > 1:91130/1‑52 (MQ=255) gctcggctcgCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGc < 1:1329056/70‑1 (MQ=255) ctcggctcgCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGcc < 1:355641/70‑1 (MQ=255) ctcggctcgCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGcc < 1:2548618/70‑1 (MQ=255) ctcggctcgCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCa < 1:2217549/71‑1 (MQ=255) tcggctcgCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCAt > 1:2977192/1‑71 (MQ=255) cggctcgCAGGAACGGGCTCACCTGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGcctt > 1:1584289/1‑68 (MQ=255) ggctcgCAGGAACGGGCTCCCCAGAACGCGTTCGATTTCCACTTTTTGa > 1:2639388/1‑49 (MQ=255) ggctcgCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTc > 1:4388284/1‑64 (MQ=255) gctcgCAGGAGCGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAg < 1:1192828/71‑1 (MQ=255) gctcgCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAg > 1:3035706/1‑71 (MQ=255) ctcgCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAgg > 1:1050222/1‑71 (MQ=255) gCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCga < 1:4437549/71‑1 (MQ=255) gCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCga < 1:4501313/71‑1 (MQ=255) aGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATc > 1:2701899/1‑63 (MQ=255) aGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATc > 1:2943856/1‑63 (MQ=255) aGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATc > 1:5104278/1‑63 (MQ=255) aGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCgaga < 1:1329409/71‑1 (MQ=255) ggAACGGGCTCACCAGAACTCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGAt < 1:1202632/71‑1 (MQ=255) ggAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGAt > 1:4675196/1‑71 (MQ=255) ggAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGAt < 1:4977131/71‑1 (MQ=255) ggAACGGGCTCACAAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGAt > 1:2760003/1‑71 (MQ=255) gAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGAt < 1:4047496/70‑1 (MQ=255) gAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGAt < 1:3430042/70‑1 (MQ=255) aaCGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTc < 1:1466486/71‑1 (MQ=255) aaCGGGCGCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTc < 1:3104991/71‑1 (MQ=255) aCGGGCTCACCAGAACGCGTTCGATTTCCACttttt > 1:3185888/1‑36 (MQ=255) aCGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCg < 1:818436/71‑1 (MQ=255) aCGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCg < 1:2699372/71‑1 (MQ=255) | AACTTCTGCACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCG > W3110S.gb/2462034‑2462172 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |