| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,505,539 | A→G | 8.6% | K475E (AAA→GAA) | ypdA → | predicted sensory kinase in two‑component system with YpdB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,505,539 | 0 | A | G | 8.6% | 294.1 / 17.1 | 105 | K475E (AAA→GAA) | ypdA | predicted sensory kinase in two‑component system with YpdB |
| Reads supporting (aligned to +/- strand): ref base A (53/43); new base G (2/7); total (55/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.24e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 121 total aligned reads displayed.
CATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCA > W3110S.gb/2505469‑2505609 | cATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTa < 1:4711996/71‑1 (MQ=255)cATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTa < 1:2886216/71‑1 (MQ=255) aTTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTaa < 1:770947/71‑1 (MQ=255) cccAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTgaa > 1:3224900/1‑69 (MQ=255) cccAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTaa > 1:2479867/1‑68 (MQ=255) cccAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGAAAAGGTgaa > 1:1876875/1‑69 (MQ=255) ccAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGc > 1:1639884/1‑71 (MQ=255) tgctgATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGtt < 1:5064970/68‑1 (MQ=255) tgctgATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGtt < 1:3582147/68‑1 (MQ=255) ctgATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTg > 1:3515771/1‑67 (MQ=255) ctgATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTc < 1:2663751/69‑1 (MQ=255) ctgATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCAc > 1:2266379/1‑71 (MQ=255) ctgATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCAc > 1:3797328/1‑71 (MQ=255) ctgATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCAc > 1:4771473/1‑71 (MQ=255) aTCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCAt > 1:4672200/1‑71 (MQ=255) ccAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCAt > 1:3782373/1‑69 (MQ=255) ccAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCAt > 1:3725767/1‑69 (MQ=255) gCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCg > 1:3591483/1‑71 (MQ=255) ccGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGt > 1:1477306/1‑71 (MQ=255) gTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTg > 1:2456768/1‑71 (MQ=255) tggtggAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTg > 1:1551074/1‑69 (MQ=255) tggtggAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTg > 1:4482162/1‑69 (MQ=255) ggtggAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTaa > 1:2004428/1‑45 (MQ=255) ggtggAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTaa > 1:595534/1‑45 (MQ=255) ggtggAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTg > 1:3856671/1‑68 (MQ=255) ggtggAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGct < 1:3522561/70‑2 (MQ=255) tggAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTGAAGGCg < 1:3422293/48‑1 (MQ=255) tggAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTGAAGGCg < 1:3678974/48‑1 (MQ=255) tggAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCAc < 1:3927648/55‑1 (MQ=255) ggAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTaaa > 1:2387213/1‑43 (MQ=255) ggAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGt > 1:578425/1‑71 (MQ=255) gagaACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTa < 1:2463724/40‑1 (MQ=255) gagaACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGt > 1:2328255/1‑70 (MQ=255) gagaACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTg > 1:4464694/1‑71 (MQ=255) agaACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGc > 1:4167781/1‑71 (MQ=255) aaCGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGt > 1:2649226/1‑59 (MQ=255) aaCGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCg > 1:17789/1‑70 (MQ=255) aCGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTa > 1:2534894/1‑36 (MQ=255) cGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCa > 1:2251108/1‑53 (MQ=255) cGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCa > 1:3941025/1‑53 (MQ=255) cGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCa > 1:505184/1‑53 (MQ=255) ccATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAAt < 1:1735130/71‑1 (MQ=255) ccATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAAt < 1:2960843/71‑1 (MQ=255) cATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGtt < 1:279389/40‑1 (MQ=255) cATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATc < 1:4796231/71‑1 (MQ=255) aTTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATca < 1:3967067/71‑2 (MQ=255) aTTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCg < 1:830307/71‑1 (MQ=255) aTTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCg < 1:3912167/71‑1 (MQ=255) aTTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCg < 1:3894769/71‑1 (MQ=255) ttGTCCACGGTATTCAGCCTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCa < 1:2354292/56‑1 (MQ=255) ttGTCCACGGTATTCAGCCTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCa < 1:4861348/56‑1 (MQ=255) ttGTCCACGGTATTCAGCCTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCa < 1:3393245/56‑1 (MQ=255) tGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCggg > 1:2194590/1‑71 (MQ=255) tGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCggg > 1:262567/1‑71 (MQ=255) gTCCACGGTATTCAGCCTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCaga > 1:2157990/1‑56 (MQ=255) gTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGt > 1:1023524/1‑35 (MQ=255) gTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGa > 1:4971468/1‑63 (MQ=255) gTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCggg > 1:2081829/1‑70 (MQ=255) gTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCggg < 1:3773275/70‑1 (MQ=255) gTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCggg < 1:4075900/70‑1 (MQ=255) tCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTa < 1:3301425/71‑1 (MQ=255) cACGGTATTCAGCCTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACg < 1:2854572/71‑1 (MQ=255) cACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACg < 1:855583/71‑1 (MQ=255) cACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACg < 1:4040410/71‑1 (MQ=255) cACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACg < 1:3391527/71‑1 (MQ=255) cACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACg < 1:1777198/71‑1 (MQ=255) cACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACg < 1:2220585/71‑1 (MQ=255) cACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACg < 1:3104671/71‑1 (MQ=255) cGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCa > 1:51657/1‑71 (MQ=255) cGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCa > 1:57524/1‑71 (MQ=255) ggTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTCCAGAGTGCGGAAATCGGGTACGCAt > 1:4849182/1‑71 (MQ=255) gTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCgg > 1:4551050/1‑62 (MQ=255) gTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCAtt > 1:1132970/1‑71 (MQ=255) gTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCAtt > 1:2854276/1‑71 (MQ=255) ttCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCa < 1:2014240/44‑1 (MQ=255) ttCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCa < 1:1435997/44‑1 (MQ=255) ttCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCg < 1:3258266/58‑1 (MQ=255) ttCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCg < 1:2774755/58‑1 (MQ=255) ttCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGc < 1:2641850/65‑1 (MQ=255) cAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCggg > 1:4419529/1‑58 (MQ=255) cAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCggg > 1:1066802/1‑58 (MQ=255) aGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCa > 1:5144613/1‑41 (MQ=255) aGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCa > 1:2087295/1‑41 (MQ=255) ccTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCg < 1:1844088/71‑1 (MQ=255) cTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCga < 1:3224961/71‑1 (MQ=255) ttGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGTAAATCGGGTACGCATTGCGGTGc > 1:2141495/1‑68 (MQ=255) gCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGaaa > 1:3395140/1‑46 (MQ=255) gCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCgaga > 1:3275914/1‑70 (MQ=255) gCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCg > 1:2508876/1‑67 (MQ=255) gCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCg > 1:2980592/1‑67 (MQ=255) cAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGAt < 1:853296/70‑1 (MQ=255) gTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACg < 1:2016447/49‑1 (MQ=255) gTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCgaga < 1:1482817/64‑1 (MQ=255) gTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCggg < 1:3271223/71‑1 (MQ=255) tAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTg < 1:4653744/53‑1 (MQ=255) tAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCggg > 1:2391394/1‑70 (MQ=255) aaaGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCga > 1:1114647/1‑60 (MQ=255) aaaGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATa > 1:228207/1‑64 (MQ=255) aaaGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATa > 1:102080/1‑64 (MQ=255) aaaGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCa > 1:3964856/1‑71 (MQ=255) | CATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCA > W3110S.gb/2505469‑2505609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |