| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,513,100 | T→C | 11.4% | N81D (AAT→GAT) | ypdG ← | predicted enzyme IIC component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,513,100 | 0 | T | C | 11.4% | 88.2 / 4.9 | 35 | N81D (AAT→GAT) | ypdG | predicted enzyme IIC component of PTS |
| Reads supporting (aligned to +/- strand): ref base T (11/20); new base C (2/2); total (13/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
CCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGA > W3110S.gb/2513030‑2513165 | ccgccgAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTAtt < 1:3634062/71‑1 (MQ=255)ccgccgAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTAtt < 1:306361/71‑1 (MQ=255)ccgccgAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTAtt < 1:1883819/71‑1 (MQ=255) gccgAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATCaa < 1:3684093/71‑1 (MQ=255) gAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAagag > 1:4610575/1‑71 (MQ=255) aCTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATcc < 1:3912924/71‑1 (MQ=255) tGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATCAAGAGCATCCat > 1:4311385/1‑71 (MQ=255) cGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCat < 1:5028565/69‑1 (MQ=255) cGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCat < 1:3030998/69‑1 (MQ=255) ggCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGAtct < 1:2378342/71‑1 (MQ=255) gCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTaa > 1:2264338/1‑49 (MQ=255) gCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGAtct > 1:3764245/1‑70 (MQ=255) gCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGAtct > 1:4197692/1‑70 (MQ=255) aaaTTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATCAAGAGCATCCATGATGCCGAtctc > 1:3358618/1‑69 (MQ=255) aaTTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTAtt < 1:3959838/44‑1 (MQ=255) tttAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCa > 1:1873178/1‑49 (MQ=255) agGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTa < 1:2298813/71‑1 (MQ=255) agGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTa < 1:306149/71‑1 (MQ=255) ggAGAGGGCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTaa < 1:4323338/71‑1 (MQ=255) gagGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGcc < 1:4778600/71‑1 (MQ=255) gagGTCGAAACCGGAGAATTTCCCGCTATCAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGcc < 1:401520/71‑1 (MQ=255) agGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCa < 1:4339443/71‑1 (MQ=255) gTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAgg < 1:4396309/57‑1 (MQ=255) gTCGAAACCGGAGAATTTCCAGCTATTAAGAGCATCCATGATGCCGATCTCAGCAgg < 1:3921021/57‑1 (MQ=255) tCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAAt < 1:2575614/59‑1 (MQ=255) tCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTaa < 1:2826729/64‑1 (MQ=255) cGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCa > 1:2566928/1‑36 (MQ=255) aaaCCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCtat < 1:1502484/70‑1 (MQ=255) ggAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCaa > 1:2550299/1‑71 (MQ=255) gagaATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAAtttt < 1:2331974/53‑1 (MQ=255) gaATTTCCCGCTATTAAGAGCATCAATGATGCCGATCTcagc > 1:2297429/1‑42 (MQ=255) aaTTTCCCGCTATTAAGAGCATCCATGATGCCGATCTcag < 1:4169918/40‑1 (MQ=255) aaTTTCCCGCTATTAAGAGCATCCATGATGCCGATCTcag < 1:168436/40‑1 (MQ=255) aaTTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAg > 1:2726670/1‑53 (MQ=255) aTTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAg < 1:3702978/70‑1 (MQ=255) gCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGa < 1:748929/71‑1 (MQ=255) tATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTa > 1:1430888/1‑41 (MQ=255) tATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAg < 1:1458141/54‑1 (MQ=255) tATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGa > 1:4599775/1‑69 (MQ=255) ttAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAAt < 1:3634658/56‑1 (MQ=255) | CCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGA > W3110S.gb/2513030‑2513165 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |