| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,533,467 | C→T | 24.0% | G47S (GGT→AGT) | ypeB ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,533,467 | 0 | C | T | 24.0% | 97.0 / 27.8 | 50 | G47S (GGT→AGT) | ypeB | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (18/20); new base T (7/5); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGATTA > W3110S.gb/2533399‑2533535 | cTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGa < 1:1907231/71‑1 (MQ=255) tCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGc > 1:2661795/1‑71 (MQ=255) ccAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCggg < 1:1768952/71‑1 (MQ=255) gCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACTGAGc < 1:2837296/53‑1 (MQ=255) gCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACTGAGc < 1:1926656/53‑1 (MQ=255) gCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTcc > 1:65453/1‑57 (MQ=255) gCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTcc > 1:237454/1‑57 (MQ=255) gCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTc > 1:2069709/1‑70 (MQ=255) tCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACTGAGCTTc < 1:3402901/53‑1 (MQ=255) cAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCtt > 1:4385573/1‑71 (MQ=255) cTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCggg < 1:2411033/71‑1 (MQ=255) cTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCg > 1:3189625/1‑69 (MQ=255) tCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCggg > 1:890692/1‑70 (MQ=255) cGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTccc > 1:1808397/1‑46 (MQ=255) cGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCg > 1:390321/1‑67 (MQ=255) ggTTTTAATCAGCAGCGTGATTTGCATTAGCTCACTGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCa > 1:5050538/1‑70 (MQ=255) ggTTTTAATCAGCAGCGTGATTTGCATTAGCTCACTGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCa < 1:1103081/70‑1 (MQ=255) ggTTTTAATCAGCAGCGTGATTTGCATTAGCTCACTGAGATTCCCTGCCGGGAACTCATCTTTGCGGGCa > 1:3692009/1‑70 (MQ=255) ggTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGACGGGAACTCATCTTTGCGGGCaa < 1:3316390/71‑1 (MQ=255) aaTCAGCAGCGTGATTTGCATTAGCTCACTGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACcac < 1:4986763/70‑1 (MQ=255) aTCAGCAGCTTGATTTGCATTAGCTCACCGAGCTTCCCTGCCg > 1:2979655/1‑43 (MQ=255) aTCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCg > 1:1564464/1‑43 (MQ=255) aTCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCg > 1:3718882/1‑43 (MQ=255) aTCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCg > 1:2831761/1‑43 (MQ=255) aTCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCg > 1:1827705/1‑43 (MQ=255) aTCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCg > 1:2541293/1‑43 (MQ=255) aTCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAAc < 1:3252389/66‑1 (MQ=255) aTCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAAc < 1:3258189/66‑1 (MQ=255) aTCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAAc < 1:1914050/66‑1 (MQ=255) aTCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCAcag < 1:2916341/71‑1 (MQ=255) aTCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCAcag < 1:2544133/71‑1 (MQ=255) agcagcGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCAcag > 1:2218603/1‑68 (MQ=255) gcagcGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGa < 1:2979501/71‑1 (MQ=255) gcagcGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGa < 1:886930/71‑1 (MQ=255) gcagcGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGa < 1:2005145/71‑1 (MQ=255) gcagcGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGa < 1:3785808/71‑1 (MQ=255) cagcGTGATTTGCATTAGCTCACTGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGa > 1:5017505/1‑70 (MQ=255) gcGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCaa < 1:2223844/57‑1 (MQ=255) gTGATTTGCATTAGCTCACTGAGCTTCCCTGCCGGGAACTCATCt > 1:922462/1‑45 (MQ=255) gTGATTTGCATTAGCTCACCGAGCTTCCCTGCCggg < 1:3669585/36‑1 (MQ=255) tGATTTGCATTAGCTCACTGAGCTTCCCTGCCGGGAACTCATCTTTGCggg > 1:327517/1‑51 (MQ=255) tttGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCAt < 1:1969232/39‑1 (MQ=255) tGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGc < 1:3117388/43‑1 (MQ=255) tGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCgg > 1:4923951/1‑71 (MQ=255) gCATTAGCTCACTGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCAcagcag > 1:2821339/1‑58 (MQ=255) gCATTAGCTCACTGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCAcagcag > 1:3159397/1‑58 (MQ=255) cACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGAt > 1:866461/1‑70 (MQ=255) aCCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGAtt < 1:2387472/70‑1 (MQ=255) aCCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGAtt < 1:3285422/70‑1 (MQ=255) aCCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGATTa < 1:3460087/71‑1 (MQ=255) ccGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGa < 1:2539775/48‑1 (MQ=255) | CTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGATTA > W3110S.gb/2533399‑2533535 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |