| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,609,011 | T→C | 46.7% | N64N (AAT→AAC) | hyfH → | hydrogenase 4, Fe‑S subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,609,011 | 0 | T | C | 46.7% | ‑0.9 / 39.9 | 30 | N64N (AAT→AAC) | hyfH | hydrogenase 4, Fe‑S subunit |
| Reads supporting (aligned to +/- strand): ref base T (4/12); new base C (5/9); total (9/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
CGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTG > W3110S.gb/2608948‑2609071 | cGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCgcgc < 1:5099575/70‑1 (MQ=255)cGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCgcgc < 1:4369588/70‑1 (MQ=255) gTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCAcc < 1:1171408/71‑1 (MQ=255) cgcCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGc < 1:4078122/71‑1 (MQ=255) ccTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAACTCGCGCACCTGGCAGctc < 1:4200124/71‑1 (MQ=255) cTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAACTCGCGCACCTGGCAGctct < 1:3853418/71‑1 (MQ=255) tgCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATc < 1:3666731/71‑1 (MQ=255) tgCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATc < 1:1359492/71‑1 (MQ=255) cTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAACTCGCGCACCTGGCAGCTCTATCTg < 1:2571886/71‑1 (MQ=255) tGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAACTCGCGCACCTGGCAGCTCTATCTggg > 1:408625/1‑71 (MQ=255) tCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTgggg < 1:1245347/70‑1 (MQ=255) ccGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAAtt > 1:3334767/1‑43 (MQ=255) ccGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAAtt > 1:1081316/1‑43 (MQ=255) cGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGt > 1:2359170/1‑71 (MQ=255) aTGCTCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTAtt < 1:4685428/71‑1 (MQ=255) aTGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATc < 1:2845254/57‑1 (MQ=255) aTGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATc < 1:5102218/57‑1 (MQ=255) aTGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTAtt < 1:4408309/71‑1 (MQ=255) aTGCGCTGACTATCCAGACCGACGACCAGCAAAACTCGCGCACCTTGCAGCTCTATCTGGGGCGTTGTAtt < 1:1902449/71‑1 (MQ=255) aTGCGCTGACTATCCAGACCGACGACCAGCAAAACTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTAtt < 1:2976707/71‑1 (MQ=255) cAGCCCGACGACCAGCAAAACTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGtt > 1:4408708/1‑70 (MQ=255) cAGACCGACGACCAGCAAAACTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTtg > 1:4273712/1‑71 (MQ=255) gACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTtgtg < 1:2225819/71‑1 (MQ=255) cccGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTtgt > 1:2221222/2‑69 (MQ=255) ccGACGGCCAGCAAAACTCGCGCACCTGGCAGCTCTa > 1:661011/1‑37 (MQ=37) ccGACGACCAGCAAAACTCGCGCACCTGGCAGCTCTa > 1:3811840/1‑37 (MQ=255) gacgacCAGCAAAACTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTgaaga < 1:1701974/71‑1 (MQ=255) gacgacCAGCAAAACTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTgaaga < 1:2005605/71‑1 (MQ=255) acgacCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGc > 1:2135303/1‑42 (MQ=255) ccAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTg > 1:3566639/1‑51 (MQ=255) ccAGCAAAACTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAgtg < 1:4303963/70‑1 (MQ=255) gCAAAACTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTa < 1:983556/41‑1 (MQ=255) | CGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTG > W3110S.gb/2608948‑2609071 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |